Very Low Frequency EPR Imaging in Vivo Phisiology P41 EB02034

描述(申请人提供)：分子氧合图像的高分辨率图像可以为癌症治疗的提供和监测提供重要的指导。氧的EPR成像(EPRI)提供了空间分辨率、氧分辨率和对组织深度的一致灵敏度的独特组合。该中心专注于体内EPRI氧气成像的优化，以预测人体图像。它是由芝加哥大学、马里兰大学和丹佛大学组成的联盟。成功的图像需要协调发展的仪器设备来获得图像，旋转探头来采样和报告组织液环境，以及成像策略来最佳地采样和分析所获得的信息。该中心建立在工程师、生物学家、放射肿瘤学家、医学物理学家、物理学家和有机化学家、统计学家和成像数学家的多学科努力的基础上。在过去的21年半里，该中心1)逐点验证EPRI氧气图像，2)开发氧与解剖学和其他生理学的联合配准，3)增加动物肿瘤图像，4)产生第一个比连续波(CW)采集快近一个数量级的电子自旋回波氧图像，5)开发出能够大量合成的窄线三叔丁基自旋探针，6)合成持久的细胞内氮氧化物，7)从允许部分偶数维投影的投影中彻底改变图像重建。这些成果直接提高了空间、氧合和/或时间图像的分辨率。生理上相关的分辨率目标要求提高EPRI小动物氧气图像的分辨率。这一改进对于大规模成像更大体积的组织和肿瘤是必要的。我们建议： 1)实现多种连续波捕获技术，以提高投影捕获速率、选择感兴趣区域和构建新的谐振器 2)对于脉冲图像采集，增加了带宽和带宽均匀度以及新的谐振器和选择感兴趣区域的技术 3)开发新的、更窄的线自旋探针，以具有更高的氧灵敏度 4)在图像采集和分析进展的基础上。 我们希望在支持的第五个年头获得一个人的形象。

DESCRIPTION (provided by applicant): High resolution images of molecular oxygenation images can provide crucial guides to the delivery and monitoring of cancer therapy. EPR imaging (EPRI) of oxygen provides a unique combination of spatial resolution, oxygen resolution, and uniform sensitivity with depth in tissue. This Center focuses on the optimization of In Vivo EPRI oxygen imaging anticipating human images. It is a consortium between the Universities of Chicago, Maryland and Denver. Successful images require coordinated development of instrumentation to obtain the images, spin probes to sample and report the tissue fluid environment and imaging strategies to optimally sample and analyze the information obtained. The Center is built on multidisciplinary effort from engineers, biologists, radiation oncologists, medical physicists, physical and organic chemists, statisticians and imaging mathematicians. In the past 21/2 years the Center 1) validated EPRI oxygen images point by point, 2) developed co-registration of oxygen with anatomy and other physiology, 3) increased animal tumor size imaged, 4) produced the first electron spin echo oxygen image nearly an order of magnitude faster than continuous wave (CW) acquisition, 5) developed a source of narrow line trityl spin probe capable of bulk synthesis, 6) synthesized persistent intracellular nitroxides and 7) radically altered reconstruction of images from projections allowing partial projections in even dimensions. These achievements directly improve spatial, oxygenation and/or temporal image resolution. Physiologically relevant resolution goals mandates improvement in resolution of EPRI oxygen images for small animals. This improvement will be necessary to scale to imaging larger volumes of tissue and tumor. We propose to: 1) Implement multiple techniques for CW acquisition to increase projection acquisition rate, select regions of interest and construct novel resonators 2) For pulsed image acquisition increase bandwidth and bandwidth uniformity as well as novel resonators and techniques to select regions of interest 3) Develop novel, even narrower line spin probes to with higher oxygen sensitivity 4) Build on the image acquisition and analysis progress. We hope to acquire a human image in the 5th year of support.