Dietary copper reconfigures pathogen growth

膳食铜重新配置病原体生长

基本信息

批准号：
9796793
负责人：
Andreas J Baumler
金额：
$ 19.63万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-06-06 至 2021-05-31
项目状态：
已结题

项目摘要

Project Summary The vertebrate large intestine is host to a large microbial community that confers benefit by functioning in host nutrition, immune education and colonization resistance against enteric pathogens. The composition of the gut microbiota varies with the diet, but little is known about how shifts in the microbiota composition affect functionality, such as the ability to confer colonization resistance against enteric pathogens. Here we will investigate how dietary copper supplementation, a common practice to improve growth performance in livestock, alters colonization resistance against the enteric pathogen Salmonella enterica serovar (S.) Typhimurium. Our central hypothesis is that dietary copper supplementation depletes propionate- producing Bacteroidaceae from the gut microbiota, thereby conferring a fitness advantage for S. Typhimurium strains carrying the sopE gene. We will test key aspects of our hypothesis and accomplish the objectives of this application using the logical and innovative approach outlined in the following specific aim: Determine whether a dietary copper-induced Bacteroidaceae depletion lowers colonization resistance against S. Typhimurium lysogenized with a sopE-encoding prophage. It is our expectation that the outcome of our experiments will show that diet-induced shifts in the gut microbiota composition in livestock can select for enteric pathogens carrying new virulence factors, which is relevant for human health because livestock carriage is a common route for introducing the pathogen into our food supply. This outcome will be significant because it will usher in the novel concept that diet-induced shifts in the microbiota composition can select for new virulence factors in enteric pathogens, a paradigm of broad interest to researchers in bacterial pathogenesis, microbiota and nutrition.

项目摘要脊椎动物大肠是一个大型微生物群落的主机，该社区受益于在宿主营养，免疫教育和对肠子的殖民化抗性中发挥作用病原体。肠道菌群的组成随饮食而异，但对微生物群组成的变化如何影响功能，例如赋予功能对肠道病原体的定殖抗性。在这里，我们将研究饮食铜如何补充是改善牲畜增长绩效的常见做法，改变了对肠道病原体沙门氏菌血清的定殖抗性（S.）伤寒。我们的核心假设是，饮食中补充饮食含量耗尽了丙酸从肠道菌群中产生拟菌科，从而为S提供了健身优势。携带SOPE基因的伤寒菌株。我们将检验假设的关键方面使用逻辑和创新的方法来实现此应用程序的目标以下特定目的：确定饮食铜诱导的菌科的耗竭是否降低对鼠伤寒链霉菌的抗性，用sope编码预言。我们期望我们实验的结果表明饮食引起的牲畜中肠道菌群组成的变化可以为携带新的肠道病原体选择毒力因子，这与人类健康有关，因为牲畜运输是常见的将病原体引入我们的食物供应的途径。这个结果将是重要的，因为它将迎来一个新的概念，即饮食引起的微生物群体组成的转移可以选择对于肠道病原体中的新毒力因素，研究人员广泛关注细菌发病机理，菌群和营养。