ShEEP Request for a Perkin Elmer Quantum GX2 Micro CT Imaging System

通过此申请，我们要求从ORD共享设备评估计划中获得资金 （ShEEP）购买活体动物微型CT扫描仪作为共享资源， 奥马哈退伍军人医疗中心的研究服务。具体来说，我们想得到一个珀金埃尔默 Quantum GX 2微型CT高分辨率台式体内显微断层扫描仪。Quantum GX 2允许 用于活体啮齿动物物种的快速、高分辨率全身成像。Quantum GX2将提供 奥马哈退伍军人目前没有的最先进的活体动物成像能力 事务医学中心（VAMC）校园或其学术附属机构，内布拉斯加大学医学院 中心（联合国监测委员会）。该设备将为现有的Omaha VA共享成像添加新功能 设备包括以前由ShEEP奖购买的IVIS Lumina K系列III系统 以及用于身体成分测定的Echo MRI成像系统。因此，量子 GX2微型CT成像系统将显着提高奥马哈VA的研究能力 调查员以及吸引新的合作与附属调查员。Quantum GX 2是一款 高性能台式体内微型CT，具有可变放大率水平，用于扫描小 实验动物以及离体组织。该设备具有高分辨率，大图像尺寸， 用于重建的圆形和螺旋扫描以及低剂量成像。由于大 在Quantum GX 2的图像视野中，可以执行全身小鼠和大鼠扫描。的 可变放大率允许以高空间分辨率扫描骨骼和组织样本， 2.3µm像素大小。一系列过滤器允许从肺组织到 骨头此外，Quantum GX 2体内微型CT对动物的辐射剂量较低 允许多次纵向扫描而没有辐射引起的副作用。Quantum GX 2 能够重建3D图像和定量计算组织形态。 重要的是，Quantum GX 2微型CT扫描仪针对纵向分析进行了优化， 直接适用于在OVAMC进行的研究，这些研究正在研究功能 和解剖学基础的疾病是直接相关的使命的VA。量子 GX 2微型CT成像系统具有灵活性，可满足所有列出的实验需求。 参与本申请的研究者。这种成像系统为两种成像系统都创造了新的能力。 奥马哈VAMC以及我们的附属大学（UNMC），从而创造了新的能力， 目前不存在。量子GX 2将允许新的方法来分析我们的研究人员现有的 退伍军人相关的临床前疾病模型，从而促进对疾病的理解 病理生理学，希望推进治疗干预，将支持的使命， 促进今天退伍军人的医疗保健。

With this application we request funds from the ORD Shared Equipment Evaluation Program (ShEEP) to purchase a live animal micro CT scanner as a shared resource to be housed in the Research Service of the Omaha VA Medical Center. Specifically, we seek to obtain a Perkin Elmer Quantum GX2 micro CT high-resolution desktop in vivo microtomograph. The Quantum GX2 allows for rapid, high-resolution full body imaging of live rodent species. The Quantum GX2 will provide state-of-the art in vivo live animal imaging capabilities not currently available on the Omaha Veterans Affairs Medical Center (VAMC) campus or its academic Affiliate, the University of Nebraska Medical Center (UNMC). This device will add new capabilities to the existing Omaha VA shared imaging equipment consisting of an IVIS Lumina K Series III system previously purchased by a ShEEP award as well as an Echo MRI imaging system for body composition determinations. As such, the Quantum GX2 micro CT imaging system will significantly enhance the research capabilities of both Omaha VA investigators as well as attract new collaborations with affiliate investigators. The Quantum GX2 is a high performance desktop in vivo micro-CT with variable magnification levels for scanning small laboratory animals as well as ex vivo tissues. This device features high resolution, large image size, both round and helical scanning for reconstruction, and low dose imaging. Because of the large image field of view of the Quantum GX2, full body mouse and rat scanning can be performed. The variable magnification allows scanning bone and tissue samples with high spatial resolution down to 2.3 µm pixel size. A range of filters allows for diverse tissue density applications from lung tissue to bone. Further, the Quantum GX2 in vivo micro CT administers low radiation dose to the animals allowing multiple longitudinal scans without radiation-induced side effects. The Quantum GX2 enables reconstruction of 3D-images and the quantitative calculation of tissue morphometry. Importantly, the Quantum GX2 micro CT scanner is optimized for longitudinal analyses, which is directly applicable to the studies being performed at the OVAMC that are investigating the functional and anatomical basis for diseases that are directly relevant to the mission of the VA. The Quantum GX2 micro CT Imaging System has the flexibility to serve the experimental needs of all the listed participating investigators in this application. This imaging system creates new capability for both the Omaha VAMC as well as our Affiliate University (UNMC), and thus creates new capacity that does not currently exist. The Quantum GX2 will allow for new ways of analyzing our researchers’ existing Veteran-relevant, preclinical disease models, thus promoting the understanding of disease pathophysiology in hopes to advance the therapeutic interventions that will support the mission of the VA in advancing the health care of today’s Veterans.