ShEEP Request for CTL ImmunoSpot S6 Universal Analyzer

ShEEP 请求 CTL ImmunoSpot S6 通用分析仪

• 批准号: 9795713
• 负责人: Chinthalapally V. Rao
• 金额: --
• 依托单位: OKLAHOMA CITY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795713
• 关键词: Alzheimer' s Disease; Antineoplastic Agents; Appointment; Area; Arthritis; B-Lymphocytes; Biological Assay; Cancer Center; Carcinoma; Cell Communication; Cells; Cellular biology; Chronic; Chronic Disease; Cities; Color; Communities; Complex; Cost of Illness; Cytotoxic T-Lymphocytes; Defense Mechanisms; Degenerative polyarthritis; Detection; Development; Disease; Disease Progression; Flow Cytometry; Funding; Future; Grant; Health Sciences; Heart Diseases; Hepatitis; Heterogeneity; Image Analysis; Immune; Immunologic Markers; Immunologic Monitoring; Immunologics; Immunology; Immunooncology; Infection; Infiltration; Inflammation; Inflammatory; Inflammatory Bowel Diseases; Institution; Leadership; Maintenance; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Measures; Mediating; Medical center; Methodology; Microscopic; Minor; Molecular; Monitor; Nature; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Oklahoma; Pancreatitis; Pathologic; Peer Review; Performance; Peripheral Blood Mononuclear Cell; Play; Preventive; Reader; Research; Research Activity; Research Personnel; Resources; Role; Sample Size; Scientist; Sheep; Surveys; T-Lymphocyte; Technology; Time; Tissues; United States; United States National Institutes of Health; Universities; Veterans; antigen-specific T cells; base; career; cell type; cellular targeting; cytokine; design; drug development; enzyme linked immunospot assay; flexibility; instrument; interest; macrophage; particle; prevent; ranpirnase; research facility; tool; tumor

We are requesting funds to purchase a CTL ImmunoSpot® S6 Universal Analyzer. This instrument allows investigators primarily to perform multi-color ELISPOT analysis along with other cell- based applications. Currently, neither the research facility at the Oklahoma City VA Medical Center (OKC VAMC) nor the affiliated University of Oklahoma Health Sciences Center has this high-end ELISPOT analyzer. It is well known that immune cells play a critical role in many diseases including cancer. It is now becoming evident that changes in immune cell heterogeneity potentially impacts either positively or negatively on the disease progression. However, in the past only limited technology allowed investigators to study heterogeneity of immune cells, immune cells interaction with other cell types and their molecular impact in the tissue microenvironment and more so, immunological markers interface in tissue microenvironment and systemically. Immune monitoring is complex and better understanding requires multiplexing. CTL ImmunoSpot® S6 Universal Analyzer permit multicolor T and B cell fluorospot analysis, live/dead PBMC counting, and much more. This is an attractive alternative to flow cytometry analysis. CTL ImmunoSpot® S6 Universal Analyzer also allows investigators for other applications at the microscopic (single cells and subcellular particles) as well as the macroscopic level (colonies and Plaques). The primary field of application for CTL ImmunoSpot® S6 Universal Analyzer has been multi-color ELISPOT analysis. CTL employs a technology specifically designed to avoid excitation cross-contamination, enabling the accurate detection of up to eight analyte co-expressing cells, including polyfunctional T cells. In addition, image analysis-based multi-parameter analysis of cells with CTL ImmunoSpot® S6 Universal Analyzer can be useful with both adherent or non-adherent cells alike, and the sample size for analysis can be as low as one cell. A survey of all VA researchers at OKC VAMC showed over 80% of the researchers are potentially interested to use CTL ImmunoSpot® S6 Universal Analyzer to study the modulation of antigen- specific T cell induction and activation in their research. This instrument will be used by six major users who have VA Merit grants funded or pending. We also anticipate that researchers at other VA centers will use this instrument.

我们要求资金购买CTLImmunoSpot®S6通用分析仪。这个乐器 允许研究人员主要可以进行多色ELISPOT分析以及其他细胞 基于应用程序。目前，俄克拉荷马城弗吉尼亚州医疗的研究机构均未 中心（OKC VAMC）或俄克拉荷马州会员大学健康科学中心已有 这款高端ELISPOT分析仪。众所周知，免疫细胞在许多人中起着至关重要的作用 包括癌症在内的疾病。现在正在证明免疫电池异质性的变化 可能对疾病进展产生积极或负面影响。但是，过去 只有有限的技术才允许研究人员研究免疫细胞的异质性，免疫 细胞与其他细胞类型的相互作用及其在组织微环境中的分子影响 更重要的是，在组织微环境和系统中的免疫标记物接口。 免疫监测是复杂的，更好的理解需要多路复用。 CTL Immunospot®S6通用分析仪允许多色T和B细胞荧光组分析， 活/死PBMC计数等等。这是流式细胞仪的有吸引力的替代方法 分析。 CTLImmunospot®S6通用分析仪还允许研究人员 在微观（单细胞和亚细胞颗粒）以及宏观上的应用 水平（殖民地和斑块）。 CTLImmunospot®S6通用申请的主要领域 分析仪是多色ELISPOT分析。 CTL员工专门 旨在避免兴奋的交叉污染，可准确检测多达八个 分析物共表达细胞，包括多功能T细胞。此外，基于图像分析 用CTLImmunospot®S6通用分析仪对细胞的多参数分析可以有用 使用粘附或非粘附细胞，以及用于分析的样本量 一个单元。 OKC VAMC的所有VA研究人员的调查显示了超过80％的研究人员 有兴趣使用CTLImmunoSpot®S6通用分析仪研究抗原的调节 特定的T细胞诱导和研究中的激活。该乐器将由六个专业使用 拥有VA功绩赠款的用户资助或待处理。我们还期望其他研究人员在其他 VA中心将使用此工具。