ShEEP Request for a Confocal Laser Scanning Microscope

ShEEP 请求共焦激光扫描显微镜

• 批准号: 9795888
• 负责人: JEROME M SIEGEL
• 金额: --
• 依托单位: VA GREATER LOS ANGELES HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795888
• 关键词: Affect; Aging; Basic Science; Body Size; Calcium-Binding Proteins; Cells; Clinical Research; Computer software; Core Facility; Detection; Dimensions; Elements; Ensure; Enzymes; Epilepsy; Equipment; Experimental Models; Fluorescence; Funding; Future; Genetic; Goals; Grant; Healthcare Systems; Hour; Image; Impaired cognition; Interruption; Label; Lasers; Light; Los Angeles; Maintenance; Mental disorders; Microscope; Microscopy; Modeling; Narcolepsy; Nature; Neurodegenerative Disorders; Neuroglia; Neurons; Opiate Addiction; Optics; Output; Oxidative Stress; Parvalbumins; Pathologic; Pharmacological Treatment; Phenotype; Presynaptic Terminals; Program Reviews; Quality of life; Quantitative Microscopy; Recording of previous events; Research; Research Personnel; Research Project Grants; Resolution; Resources; Restless Legs Syndrome; Scanning; Schedule; Sheep; Site; Sleep disturbances; Sleeplessness; Source; Stains; System; Tissues; United States National Institutes of Health; Veterans; cell type; improved; inflammatory marker; innovation; nervous system disorder; neuronal cell body; nitrosative stress; oxidative damage; population health; pre-clinical research; programs

Project Summary/ Abstract This ShEEP request is for the acquisition of a confocal laser scanning microscope for the core facility at VA Greater Los Angeles Healthcare System, Sepulveda campus, which will be used by multiple investigators in building-7. These investigators are actively engaged in pre-clinical research on a range of neurological and psychiatric disorders that are highly prevalent in Veterans. One of the key component of their research programs involves double- and triple- immunostaining and analyses of the cell types that are affected to validate the nature of the morphometric, immunohistochemical, and pathological changes in those cells that underlie/contribute to those neurological and psychiatric disorders including insomnia, opiate addiction, narcolepsy, cognitive impairment, seizure disorders, and sleep disturbance in aging. As compared to the standard fluorescent microscopy, the procurement of this confocal microscope will significantly improve the ability of these investigators to precisely document and analyze those cellular and subcellular pathophysiological changes that underlie those neurological and psychiatric disorders. It will also serve as a valuable resource for the future VA investigators. Therefore, the potential of this ShEEP proposal to impact the clinical and basic research projects and therein the health of the population served by the Department of Veterans Affairs is significant.

项目摘要/摘要 这份SHEEP申请是为核心购置一台共聚焦激光扫描显微镜。 位于退伍军人事务部大洛杉矶医疗系统的塞普尔韦达园区的设施，将由 7号楼有多名调查人员。这些研究人员积极从事临床前研究 对一系列神经和精神疾病的研究，这些疾病在 退伍军人。他们研究计划的关键组成部分之一涉及双倍和三倍- 对受影响的细胞类型进行免疫染色和分析，以验证其性质 这些细胞的形态、免疫组织化学和病理变化 导致神经和精神疾病，包括失眠、鸦片类药物 成瘾、发作性睡病、认知障碍、癫痫发作障碍和老年睡眠障碍。 与标准荧光显微镜相比，该共焦显微镜的采购 显微镜将显著提高这些研究人员精确记录和记录 分析这些细胞和亚细胞的病理生理变化 神经和精神障碍。它还将成为未来退伍军人管理局的宝贵资源 调查人员。因此，这一SHEEP提案的潜力将影响临床和基础 研究项目和其中退伍军人事务部服务的人口的健康 这件事意义重大。