ShEEP Request for Laser Scanning Confocal Microscopy Acquisition and Analysis System

ShEEP 请求激光扫描共焦显微镜采集和分析系统

• 批准号: 9795294
• 负责人: Richard Altschuler
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795294
• 关键词: 3-Dimensional; Affect; Aging; Air; Algorithms; Anatomy; Applications Grants; Award; Biopsy Specimen; Central Nervous System Infections; Chronic Obstructive Airway Disease; Chronic lung disease; Cochlea; Collaborations; Computer software; Confocal Microscopy; Data; Data Analyses; Data Collection; Detection; Disease; Effectiveness; Elements; Equilibrium; Equipment; Faculty; Firearms; Fluorescence Resonance Energy Transfer; Funding; Future; Glycerol; Golf; Grant; Healthcare Systems; Hearing; Height; Image; Image Analysis; Image Enhancement; Imagery; Immersion Investigative Technique; Immunotherapy; In Situ; Individual; Infection; Label; Labyrinth; Laser Scanning Confocal Microscopy; Lasers; Learning; Measurement; Michigan; Microscope; Molecular; Nerve Degeneration; Neurodegenerative Disorders; Noise; Normal tissue morphology; Oils; Organ; Pathogenesis; Pathologic; Pathologic Processes; Pathology; Patients; Population; Post-Traumatic Stress Disorders; Preparation; Process; Research; Research Institute; Research Personnel; Resolution; Rotation; Running; Scanning; Services; Sheep; Site; Specimen; Surface; System; Systems Analysis; Testing; Therapeutic Intervention; Thick; Three-dimensional analysis; Time; Tissue Preservation; Tissues; Toxic effect; Toxicant exposure; Training; United States National Institutes of Health; Universities; Veterans; War; Water; cancer type; chromophore; design; detector; experience; hearing impairment; high resolution imaging; image visualization; improved; live cell imaging; neoplasm immunotherapy; ranpirnase; response; ribbon synapse; systems research; targeted treatment; tissue repair; tumor growth

This proposal is for a shared Laser Scanning Confocal Microscopy Acquisition and Analysis System to allow a Major User group (six Merit Award funded PIs) as well as other VA researchers at the VA Ann Arbor Health Care System (VAAAHS) to conduct high-resolution analysis in both living and preserved tissues. Research focuses on cellular pathogenesis of diseases that preferentially affect veteran populations. Studies include firearm triggered loss of hearing and balance, Deployment-Related Chronic Lung Disease, PTSD, COPD, CNS infections and subset of neurodegenerative disorders, certain types of cancer (tumor immunotherapy). These diseases are characterized by specific, and often subtle, micro-anatomic tissue changes and a detailed identification and assessment at the molecular level is critical to execute studies, propose new treatment opportunities and subsequently test their effectiveness. This need is not being adequately met. The requested system is composed of Leica SP-8 inverted laser scanning confocal microscope with a Tokia HIT stage-top incubation system for live cell imaging. It will have multiple lasers and high capability detectors with adjustable channels, rapid acquisition capacity and up to 120 nm resolution. Air, water, oil and glycerol immersion objectives with varying working distances and numerical apertures will match the needs of our diverse user group for their variety of specimens and assessments. Software on both acquisition and image analysis workstations will provide for enhanced imaging, visualization, co-localization and rotation as well as 2-D and 3- D analysis and measurement. FRET and FRAP “wizards” will allow these applications. Introducing new capabilities will enhance current studies by providing new data and improved data collection and analyses as well as increasing efficiency and throughput. It will extend current studies into new directions by allowing identifications and assessments that would not otherwise be possible, for example, by allowing new multiple co-labeling of molecules and cellular elements deep into specimens or by allowing real-time dynamic live cell imaging, FRET and FRAM analyses. It will also create new capabilities aiding future grant applications of our faculty and new learning opportunities for trainees at the VAAAHS site. The requested system will enhance and extend VAAAHS research on disorders effecting our veteran population towards developing therapeutic interventions to ultimately benefit our patients.

该提案是关于共享激光扫描共焦显微镜采集和分析系统，以允许 主要用户组（六名优异奖资助的 PI）以及 VA Ann Arbor Health 的其他 VA 研究人员 护理系统 (VAAAHS) 可对活体和保存的组织进行高分辨率分析。研究 重点研究优先影响退伍军人群体的疾病的细胞发病机制。研究包括 枪支引发听力和平衡丧失、部署相关慢性肺病、创伤后应激障碍 (PTSD)、慢性阻塞性肺病 (COPD)、中枢神经系统 (CNS) 感染和神经退行性疾病的子集，某些类型的癌症（肿瘤免疫疗法）。这些 疾病的特征是特定的、通常是微妙的微观解剖组织变化和详细的 分子水平的识别和评估对于执行研究、提出新的治疗方法至关重要 机会并随后测试其有效性。这一需求没有得到充分满足。所要求的 系统由 Leica SP-8 倒置激光扫描共聚焦显微镜和 Tokia HIT 载物台组成 活细胞成像孵化系统。它将拥有多个激光器和高性能探测器，可调节 通道、快速采集能力和高达 120 nm 的分辨率。空气、水、油和甘油浸泡 具有不同工作距离和数值孔径的物镜将满足不同用户的需求 小组的各种标本和评估。采集和图像分析软件 工作站将提供增强的成像、可视化、共定位和旋转以及 2-D 和 3- D 分析和测量。 FRET 和 FRAP“向导”将允许这些应用程序。推出新品 能力将通过提供新数据和改进的数据收集和分析来增强当前的研究 以及提高效率和吞吐量。它将通过允许将当前的研究扩展到新的方向 进行其他方式不可能实现的识别和评估，例如允许新的多重 共同标记样本深处的分子和细胞元素或通过允许实时动态活细胞 成像、FRET 和 FRAM 分析。它还将创造新的能力，帮助我们未来的拨款申请 VAAAHS 站点的师资力量和为学员提供的新学习机会。所要求的系统将增强 并扩展 VAAAHS 对影响我们退伍军人群体的疾病的研究，以开发治疗方法 干预措施最终使我们的患者受益。