ShEEP request for IVIS SpectrumCT Imaging System

在这项ShEEP资助中，我们申请资金购买临床前体内成像系统， Perkin Elmer安装在VA肌肉骨骼疾病中心的表型核心 洛马琳达医疗保健系统（VALLHCS）。Perkin Elmer的IVIS SpectrumCT是一种 集成了一流的光学成像和低剂量microCT， 和解剖纵向研究，为研究人员提供了复杂的基本见解 小动物模型中的生物系统。恒定的水平机架运动和平坦的 面板探测器为低剂量成像和自动光学和 microCT集成稳定的旋转动物平台可360度旋转， 3D数据。该系统的另一个特点是可以同时扫描多只动物 同时保持每次扫描的平均剂量为1.3戈伊，扫描和重建时间为 不到一分钟该系统还允许操作光学和microCT模态 独立地。IVIS SpectrumCT允许对动物进行真实的地形表面测绘 所包含的工作流程和成像向导功能可简化3D断层扫描程序 以及生物发光、荧光和切伦科夫发光中的二维屏蔽模式。这 VALLHCS的一些研究人员将使用新仪器进行体内小 动物影像工作。这些应用包括但不限于在以下文献中提出的实验： 各种资助的研究，将受益于这种在体内光学成像的可用性极大 系统该系统附带的软件允许自动和精确定量 所使用的各种荧光信号。该系统还允许获得高质量 用于数据文档和出版的图像。一些退伍军人事务部，国防部和国家卫生研究院资助 该VA医疗中心的研究者，包括Mohan、Lau、Rundle、Qin、Dong、Xing、Li博士 Edderkaoui和Edderkaoui需要这些设备来进行与他们资助的赠款有关的研究 应用.目前，没有用于小动物的体内成像系统可用于研究用途 在VALLHCS。成功完成受资助的研究需要所要求的仪器 与骨质疏松症、骨关节炎和听力的发病机制和治疗有关 损失，疾病是退伍军人管理局研究使命的目标领域。

In this ShEEP grant, we request funds to purchase a preclinical in vivo imaging system from Perkin Elmer for installation in the phenotype core of the Musculoskeletal Disease Center of VA Loma Linda Healthcare System (VALLHCS). The IVIS SpectrumCT from Perkin Elmer is an integrated premier optical imaging and low-dose microCT that enables simultaneous molecular and anatomical longitudinal studies, providing researchers with essential insights into complex biological systems in small animal models. The constant horizontal gantry motion and the flat panel detector provide outstanding performance for low-dose imaging and automated optical and microCT integration. The stable revolving animal platform rotates 360o enabling acquisition of full 3D data. Another feature of this system is that multiple animals can be scanned simultaneously while maintaining an average dose per scan at 1.3 Gy, with scanning and reconstruction time of less than a minute. The system also allows operation of optical and microCT modalities independently. The IVIS SpectrumCT allows for true topographic surface mapping of the animal and the included workflow and imaging wizard features facilitate procedures for 3D tomography and 2D screening modes in bioluminescence, fluorescence and Cerenkov luminescence. This new instrument will be used by a number of investigators at the VALLHCS to perform in vivo small animal imaging work. The applications include but are not limited to experiments proposed in various funded studies that will benefit immensely by the availability of this in vivo optical imaging system. The software that comes with the system allows automated and precise quantitation of the various fluorescent signals employed. The system also allows for obtaining high quality images for data documentation and publication. A number of VA, DOD and NIH funded investigators at this VA Medical Center including Drs. Mohan, Lau, Rundle, Qin, Dong, Xing, Li and Edderkaoui require this equipment to perform studies related to their funded grant applications. Currently, no in vivo imaging system for small animals is available for research use at VALLHCS. The requested instrument is needed for successful completion of funded studies that are relevant to the pathogenesis and treatment of osteoporosis, osteoarthritis and hearing loss, diseases that are target areas of the VA research mission.