The Tissue Phenotyping and Bone Metabolism Core Facility

组织表型和骨代谢核心的总体目标，由经验丰富的研究人员博士领导。 Bouxsein和Demay，是为肾脏和肌肉骨骼组织提供最高质量的评估， 以及血清和尿液生物化学，以最有效的方式。该核心将为调查人员提供 获得最先进的技术，(酌情)培训新设备的使用，并就 实验设计、方案制定、数据分析和解释。核心提供分层的、 具有基因改变的啮齿动物模型中肌肉骨骼表型的多尺度评估，以及 饮食和药物干预，允许对组织样本进行有效评估 时间和资源。总而言之，核心在支持调查人员方面卓有成效 方案项目，如所有项目使用岩心、样品的有效处理、 以及核心贡献的手稿数量。在这次更新中，核心将支持所有四个 建议的项目。在本供资期间，核心支助了所有项目，并将继续这样做 通过解决以下三个具体目标：1)提供最先进的血清和尿液 生化分析.组织学和组织形态计量学分析.骨骼成像和生物力学 评估；2)促进成本效益高、高效、综合、分级的组织评估方法 表型；以及3)通过提供动手培训、研究设计方面的专家建议、 排除新方案/探针/抗体的故障，并解释核心设施产生的结果。 拥有经验丰富的领导和技术人员，以及最先进的设备和严格的质量控制 措施，核心将通过提供以下服务来实现这些目标，包括：各种组织学 技术；定量组织形态计量学；活体骨密度计量学和身体成分；高分辨率 用微型计算机断层摄影术测量骨微结构；用臭氧对骨髓脂肪进行定量 四氧化三微计算机断层扫描；全骨和组织水平的生物力学测试；以及血清和 与骨骼和肾脏代谢有关的尿液生化。通过在核心内部进行这些评价 设施齐全，可随时保持高质量控制标准。此外，核心将提供协调的、 将及时进行评估，并将视需要协助调查人员设计研究和解释结果。 核心人员的工资和基本用品在核心预算中支付，但收取使用费 用于一些服务，以抵消核心预算中未涵盖的费用。我们发现这种混合方法，其中 工资和基本用品都由核心支付，但一些服务需要用户收费，结果是稳定 核心的运作，在项目之间公平地分担资源和分摊费用。

The overall goal of the Tissue Phenotyping and Bone Metabolism Core, led by experienced investigators Drs. Bouxsein and Demay, is to provide the highest quality assessment of kidney and musculoskeletal tissues, as well as serum and urine biochemistry, in the most efficient manner. The core will provide investigators with access to state-of-the-art technology, training in use of new devices (as appropriate) and consultation on experimental design, protocol development, data analysis and interpretation. The core provides hierarchical, multi-scale assessment of musculoskeletal phenotypes in rodent models with genetic alterations, as well as dietary and pharmacologic interventions, allowing the efficient evaluation of tissue samples, with regard to both time and resources. Altogether the core has been highly effective in supporting the investigators in the Program Project, as evidenced by the use of the core by all projects, the efficient processing of specimens, and number of manuscripts to which the core contributed. In this renewal, the core will support all four proposed projects. In the current funding period, the core supported all the projects and will continue to do so by addressing the following three specific aims: 1) Provide access to state-of-the-art serum and urinary biochemical analyses; histologic & histomorphometric analyses; skeletal imaging & biomechanical assessments; 2) Promote a cost-effective, efficient, integrated, hierarchical approach to evaluation of tissue phenotypes; and 3) Support investigators by providing hands-on training, expert advice on study design, trouble shooting of new protocols/probes/antibodies and interpretation of results generated by the Core facility. With experienced leadership and technical staff, as well as state-of-the-art equipment and strict quality control measures, the core will achieve these aims by offering the following services, including: a variety of histologic techniques; quantitative histomorphometry; in vivo bone densitometry and body composition; high resolution bone microarchitecture measurements via micro-computed tomography; marrow fat quantification via osmium tetraoxide micro-computed tomography; whole bone and tissue level biomechanical testing; and serum and urine biochemistry relating to bone and kidney metabolism. By conducting these evaluations within the core facility, high quality control standards can be readily maintained. Moreover, the core will provide coordinated, timely assessments, and will assist investigators with study design and interpretation of results, as needed. Salaries of core personnel and basic supplies are covered in the Core budget, however user fees are collected for some services to offset expenses not covered in the core budget. We find that this hybrid approach, where salaries and basic supplies are covered by the core, but some services require users fees, results in a stable operation of the Core, with equitable resource sharing and cost allocation amongst the projects.