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Molecular Cytogenetics

分子细胞遗传学

基本信息

批准号：
9792754
负责人：
Cristina Montagna
金额：
$ 6.15万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9792754
关键词：
3-Dimensional Albert Einstein Cancer Center Archives B-Lymphocytes Biopsy Blood Cells CD34 gene Cancer Center Cells Chromosome Painting Chromosome Structures Chromosome abnormality Chromosomes Chromosomes, Human, Pair 4 Cryopreservation Custom Cytogenetic Analysis Cytogenetics Cytogenetics Shared Resource DNA Data Analyses Detection Fluorescent in Situ Hybridization Formalin Freezing G-Banding Gatekeeping Generations Genes Genome Genomic DNA Genomic Segment Genomics Genomics Shared Resource Goals Human Human Genome Human Herpesvirus 4 Human Resources Image Interphase Karyotype determination procedure Liquid substance Malignant Neoplasms Maps Messenger RNA Metaphase MicroRNAs Modernization Molecular Computations Molecular Cytogenetics Molecular Genetics Morphology Mus Mutation Analysis Nitrogen Nuclear Nucleic Acids Nucleotides Paint Pap smear Paraffin Embedding Peripheral Blood Mononuclear Cell Population Preparation RNA Research Personnel Resolution Sampling Services Spectral Karyotyping T-Lymphocyte Technology Tissue Embedding Tissues Touch sensation Training Transformed Cell Line Variant biobank cancer genome cell free DNA design eosinophil established cell line genetic analysis innovation insertion/deletion mutation interest member mouse genome preservation reconstruction targeted sequencing tissue culture tool tumor whole genome

项目摘要

PROJECT SUMMARY - MOLECULAR CYTOGENETIC SHARED RESOURCE The Molecular Cytogenetic Shared Resource (MCSR) provides tools for the preparation of human and murine samples suitable for molecular genetics and cytogenetics analysis of the entire genome. The goal is to provide AECC investigators with the whole spectrum of services necessary to analyze the genome at various levels of resolution. These services include the establishment of EBV transformed cell lines, and enrichment of blood cell subtypes that can be cryopreserved and stored in liquid nitrogen. Isolation of DNA and mRNA from a variety of tissue culture samples as well as primary biopsies is also provided. A full panel of services is provided, from preparation of metaphase chromosomes to the generation of custom designed probes and hybridization for the detection of copy number and structural chromosomal alterations. The MCSR provides analyses at all cytogenetic levels of resolution from standard to spectral karyotyping, and from whole chromosome paints to locus-specific probes for unique regions of the genome. A recently developed targeted sequencing panel for the mutational analysis of caretaker and gatekeeper genes is now also offered for studies at single nucleotide resolution of the cancer genome. MCSR personnel are trained to design targeted, locus- specific probes for regions of interest to investigators. All probes are custom designed and generated in-house. The MCSR also performs hybridizations on paraffin-embedded tissues as well as image acquisition and full data analysis.

项目概述-分子细胞遗传学共享资源