GENE IMAGING SHARED RESOURCE

基因成像共享资源

• 批准号: 8113382
• 负责人: Cristina Montagna
• 金额: $ 12.06万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8113382
• 关键词: Albert Einstein Cancer Center; Cancer Center; Cancer Center Support Grant; Chromosome Painting; Cytogenetic Analysis; Epigenetic Process; Fluorescent in Situ Hybridization; Genes; Genome; Genomics; Goals; Housing; Human; Image; Label; Laboratories; Mus; Play; Research Activity; Research Personnel; Resource Sharing; Role; Sampling; Services; Spectral Karyotyping; Technology; base; design; interest; tool

The Genome Imaging Shared Resource (GISR) is a new shared resource developed since the last CCSG review. The facility provides tools for cytogenetic analysis of the entire genome as well as specific probes targeted to regions of interest. These include fluorescent in situ hybridization (FISH) with locus specific probes (LSP) generated "in house", specifically designed and labeled based upon the experimental requirements. Spectral Karyotyping (SKY) and whole chromosome painting for human and mouse samples are also part of the service. This facility was initiated in late 2004 with the recruitment of Cristina Montagna from Thomas Ried's laboratory from the NCI. The GISR is not a high-volume facility and, because it was inaugurated only recently, the scope of usage is smaller than for most AECC shared resources. However, this facility provides a very important technology and service to AECC investigators and will play a critical role in the growing genomics and, in particular, epigenetics research activities at the Center.

基因组图像共享资源(GISR)是自上一次CCSG以来发展起来的一种新的共享资源 复习一下。该设施提供了对整个基因组进行细胞遗传学分析的工具以及特定的探针 以感兴趣的区域为目标。其中包括与基因位点特异的荧光原位杂交(FISH) 内部生成的探头(LSP)，根据实验结果专门设计和标记 要求。人和小鼠样本的光谱核型(SKY)和全染色体涂色 也是这项服务的一部分。这一机制是在2004年底招募克里斯蒂娜·蒙塔尼亚时启动的 来自国家情报局的托马斯·莱德的实验室。GISR不是一个大容量的设施，因为它是 最近才启用，其使用范围比大多数AECC共享资源要小。然而， 该设施为AECC调查人员提供了非常重要的技术和服务，并将发挥关键作用 在该中心不断发展的基因组学，特别是表观遗传学研究活动中发挥重要作用。