GENE IMAGING SHARED RESOURCE

基因成像共享资源

• 批准号: 7886702
• 负责人: Cristina Montagna
• 金额: $ 13.21万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7886702
• 关键词: Albert Einstein Cancer Center; Cancer Center; Cancer Center Support Grant; Chromosome Painting; Cytogenetic Analysis; Epigenetic Process; Fluorescent in Situ Hybridization; Genes; Genome; Genomics; Goals; Housing; Human; Image; Label; Laboratories; Mus; Play; Research Activity; Research Personnel; Resource Sharing; Resources; Role; Sampling; Services; Spectral Karyotyping; Technology; base; design; interest; tool

The Genome Imaging Shared Resource (GISR) is a new shared resource developed since the last CCSG review. The facility provides tools for cytogenetic analysis of the entire genome as well as specific probes targeted to regions of interest. These include fluorescent in situ hybridization (FISH) with locus specific probes (LSP) generated "in house", specifically designed and labeled based upon the experimental requirements. Spectral Karyotyping (SKY) and whole chromosome painting for human and mouse samples are also part of the service. This facility was initiated in late 2004 with the recruitment of Cristina Montagna from Thomas Ried's laboratory from the NCI. The GISR is not a high-volume facility and, because it was inaugurated only recently, the scope of usage is smaller than for most AECC shared resources. However, this facility provides a very important technology and service to AECC investigators and will play a critical role in the growing genomics and, in particular, epigenetics research activities at the Center.

基因组成像共享资源（GISR）是自上一届CCSG以来开发的新的共享资源 审查.该设施提供了对整个基因组进行细胞遗传学分析的工具以及特异性探针 针对感兴趣的区域。这些包括用基因座特异性荧光原位杂交（FISH）， 探针（LSP）产生的“内部”，专门设计和标记的基础上，实验 要求.人类和小鼠样本的光谱核型分析（SKY）和全染色体涂染 也是服务的一部分。这一设施是2004年底征聘Cristina Montagna后启动的 来自国家癌症研究所的托马斯·里德实验室GISR不是一个高容量的设施，因为它是 虽然最近才开始使用，但使用范围小于大多数AECC共享资源。然而，在这方面， 该设施为AECC调查人员提供了非常重要的技术和服务， 在日益增长的基因组学，特别是在该中心的表观遗传学研究活动的作用。