Development of membrane anchored cytokines as novel vaccine adjuvants for the elderly

承包商将使用IL-12和GM-CSF的工程版本作为佐剂。IL-12和GM-CSF的工程化版本包括一个位于羧基末端的糖基磷脂酰肌醇(GPI)锚定，使它们能够整合到脂质双层中，如流感病毒样颗粒(VLP)。该佐剂的开发将在老年人口流感的背景下进行。具体任务包括(1)体外和(2)体内免疫学活性图谱的建立，以评估佐剂的能力。

The contractor will use engineered versions of IL-12 and GM-CSF as adjuvants. The engineered version of IL-12 and GM-CSF include a glycosyl phosphatidylinositol (GPI) anchor at the carboxyl terminal which permits their incorporation into lipid bilayers such as influenza viral like particles (VLP). The development of the adjuvant will be in the context of influenza in elderly populations. The specific tasks include the (1) in vitro and (2) in vivo establishment of an immunological profile of activity to evaluate the capability of the adjuvant.