Imaging mitochondrial heterogeneity in LKB1 mutant lung cancer
LKB1 突变肺癌线粒体异质性成像
基本信息
- 批准号:9392856
- 负责人:
- 金额:$ 7.11万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-09-01 至 2021-08-31
- 项目状态:已结题
- 来源:
- 关键词:Adenosine MonophosphateAnabolismAnionsAntibodiesApoptosisApoptoticAreaAvidityBiochemicalBiologyCancer PatientCancer cell lineCardiolipinsCell LineChemicalsComplexCoupledCouplingDataDefectDevelopmentDiagnosisDissectionElectron MicroscopyExposure toGeneticGenetically Engineered MouseGenus HippocampusGoalsHealthHeterogeneityHomeostasisHumanImageIn VitroInner mitochondrial membraneLeadLinkLongitudinal StudiesLung NeoplasmsMalignant NeoplasmsMalignant neoplasm of lungMapsMeasurementMeasuresMediatingMembrane PotentialsMitochondriaModelingMolecularMorphologyMusMutateMutationNeoplasm MetastasisNoduleNon-Small-Cell Lung CarcinomaOuter Mitochondrial MembranePathway interactionsPatientsPhosphotransferasesPhysiologicalPopulationPositron-Emission TomographyProtein Tyrosine PhosphatasePublic HealthRNA InterferenceRegulationSTK11 geneSignal PathwayStressStructureTestingTransmission Electron MicroscopyTumor Suppressor GenesTumor Suppressor ProteinsWorkXenograft procedurefield studyimaging detectionimproved outcomein vivoinhibitor/antagonistlung developmentlung tumorigenesismicroscopic imagingmitochondrial membranemouse modelmutantneoplastic cellnew therapeutic targetnovelnovel therapeuticsradiotracerrestorationsensortumortumor progressionvoltage
项目摘要
ABSTRACT
The goal of this study is to utilize PET imaging in GEMMs to perform a mechanistic study of mitochondrial
heterogeneity following inactivation of the LKB1/AMPK signaling pathway during lung tumor development.
LKB1 functions as a master kinase that regulates cellular energetics and mitochondrial function through
activation of the adenosine monophosphate activated kinase (AMPK) that is frequently mutated in cancer.
LKB1 mutations lead to inactivation of the AMPK signaling pathway resulting in severe defects in cellular
energetics and mitochondrial homeostasis. This results in highly variable mitochondrial pools within human and
mouse tumors that consist of numerous atypical mitochondria of differing size, morphology and function that
we define as mitochondrial heterogeneity,. However, little is understood at a physiological or mechanistic level
how mitochondrial heterogeneity resulting from LKB1 inactivation impact lung tumorigenesis or therapy. We
examined mitochondrial structural and functional heterogeneity in lung tumors in vivo by coupling electron
microscopy (EM) and positron emission tomography (PET) imaging of Lkb1-/- genetically engineered mouse
models (GEMMs). Using a voltage sensitive mitochondrial specific radiotracer [18F]-Fluorobenzyl-
triphenylphosphonium (FTP) we are able to measure mitochondrial membrane potential (∆Ψ) in lung tumors by
PET imaging. FTP PET imaging identified lung tumor populations with heterogeneous mitochondrial activity in
vivo. Additionally, mitochondrial defects sensitize LKB1-/- tumor cells to undergo mitochondrial outer
membrane permeabilization (MOMP) and apoptosis and we discovered the LKB1/AMPK pathway is a potential
regulator of MOMP and apoptosis through voltage dependent anion 1 (VDAC1). Lastly, as a result of a
synthetic lethal chemical screen, we identified protein tyrosine phosphatase mitochondria 1 (PTPMT1), a key
regulator of cardiolipin biosynthesis and mitochondrial integrity as a novel therapeutic target in LKB1-/- lung
cancer. We hypothesize that inactivation of the LKB1 tumor suppressor induces heterogeneity in mitochondrial
structure and function that drives lung tumor development. To test this hypothesis we will integrate PET and
EM imaging of Lkb1-/- GEMMs of lung cancer to longitudinally study mitochondrial heterogeneity at distinct
stages of lung tumor development. In Aim1 we will use FTP PET imaging to map mitochondrial heterogeneity
and dynamics in vivo during lung tumorigenesis following LKB1 loss. In Aim 2 we will identify the molecular
mechanisms by which the LKB1/AMPK pathway regulates the mitochondrial outer membrane. In Aim 3
perform an in vivo dissection of the PTPMT1-cardiolipin pathway in LKB1-/- lung tumors. We propose first-in-
field studies that will advance our fundamental understanding of mitochondrial biology and the impact of
mitochondrial heterogeneity has on promoting lung tumorigenesis. The proposed work has relevance to
human health in the areas of PET imaging based detection diagnosis of lung cancer as well as the
development of new therapies to improve outcomes for lung cancer patients.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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David B Shackelford其他文献
David B Shackelford的其他文献
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{{ truncateString('David B Shackelford', 18)}}的其他基金
In Vivo Imaging of Mitochondria Structure and Function in Therapy Resistant Lung Tumors
难治性肺肿瘤线粒体结构和功能的体内成像
- 批准号:
10747207 - 财政年份:2023
- 资助金额:
$ 7.11万 - 项目类别:
In vivo imaging of mitochondria structure and function in therapy resistant lung tumors
治疗耐药性肺肿瘤线粒体结构和功能的体内成像
- 批准号:
10866660 - 财政年份:2022
- 资助金额:
$ 7.11万 - 项目类别:
In vivo imaging of mitochondria structure and function in therapy resistant lung tumors
治疗耐药性肺肿瘤线粒体结构和功能的体内成像
- 批准号:
10522994 - 财政年份:2022
- 资助金额:
$ 7.11万 - 项目类别:
In vivo imaging of mitochondria structure and function in therapy resistant lung tumors
治疗耐药性肺肿瘤线粒体结构和功能的体内成像
- 批准号:
10649562 - 财政年份:2022
- 资助金额:
$ 7.11万 - 项目类别:
(PQ5) Imaging mitochondrial heterogeneity in LKB1 mutant lung cancer
(PQ5) LKB1 突变肺癌线粒体异质性成像
- 批准号:
9750637 - 财政年份:2016
- 资助金额:
$ 7.11万 - 项目类别:
Imaging mitochondrial heterogeneity in LKB1 mutant lung cancer
LKB1 突变肺癌线粒体异质性成像
- 批准号:
9403002 - 财政年份:2016
- 资助金额:
$ 7.11万 - 项目类别:
Imaging mitochondrial heterogeneity in LKB1 mutant lung cancer
LKB1 突变肺癌线粒体异质性成像
- 批准号:
10585832 - 财政年份:2016
- 资助金额:
$ 7.11万 - 项目类别:
(PQ5) Imaging mitochondrial heterogeneity in LKB1 mutant lung cancer
(PQ5) LKB1 突变肺癌线粒体异质性成像
- 批准号:
10063382 - 财政年份:2016
- 资助金额:
$ 7.11万 - 项目类别:
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