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The Evaluation of TLT-1 as treatment for ALI/ARDS

TLT-1治疗ALI/ARDS的评价

基本信息

批准号：
9450382
负责人：
Jessica Morales-Ortiz
金额：
$ 1.89万
依托单位：
UNIVERSITY OF PUERTO RICO RIO PIEDRAS
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-01-13 至 2018-08-15
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9450382
关键词：
Accounting Actins Acute Acute Lung Injury Adult Respiratory Distress Syndrome Alpha Granule Alveolitis Antibodies Attenuated Binding Biological Process Blood Blood Platelets Cell Communication Cells Clinical Clinical Trials Coronary Arteriosclerosis Cytoplasmic Granules Data Development Disease Disease Management Dissection Edema Endothelial Cells Endothelium Evaluation Exhibits Fluorescein-5-isothiocyanate Functional disorder Healthcare Hemorrhage Hemostatic Agents Hemostatic function Immune response Immune system Incubated Inflammation Inflammatory Inflammatory Response Knockout Mice Knowledge Label Laboratory Study Lead Leukocytes Life Link Lipopolysaccharides Lung Lung Inflammation Lung diseases Maintenance Mediating Mediation Medical Megakaryocytes Membrane Modeling Molecular Mus Myeloid Cells P-Selectin Pathology Patients Phenotype Property Proteins Proto-Oncogene Proteins c-akt Publishing Pulmonary Edema Reaction Recombinants Reporting Research Respiratory Failure Role Selectins Signal Transduction Site Testing Therapeutic Therapeutic Effect Transcript Trauma patient Vasculitis Work alternative treatment base controlled release cost economic impact immunoregulation in vivo interest mortality mouse model neutrophil novel therapeutic intervention novel therapeutics polymerization prevent receptor response septic patients targeted treatment tool

项目摘要

Project Summary Acute Lung injury especially acute respiratory distress syndrome (ARDS) have been identified as life threatening conditions associated with significant mortality rate. Despite advances in the past decades in the knowledge on lung diseases, ARDS continues to claim the lives of more than 40% of its victims. The increase in health care associated costs has a major economic impact, while major clinical efforts to manage this disease are insufficient due to an uncomplete understanding of the mechanisms that control the pathology manifestations. Blood platelets, neutrophils and endothelial cells have been identified as the key components in the progression of ALI/ARDS. Moreover, our understanding of platelets function has shifted over the past years from a simple hemostatic tool to dynamic modulators of the immune response. The current paradigm of platelets intents to uncover the molecular mechanisms that make these cells major orchestrators of inflammation. Previous reports have demonstrated that the absence of platelets leads to increase endothelial damage, aberrant neutrophil function and hemorrhage during inflammation. Moreover, these effects have been shown to be controlled by the release of platelet granules during activation. To date, the identity of the platelet granule molecule or molecules involved in this remains to be elucidated representing gab in our knowledge. Accordingly, this study seeks to identify key platelet components that modulate systemic response during inflammation through the modulation of neutrophil-endothelial cell crosstalk and mechanisms involved. To dissect that question we will evaluate a platelet granule product known as TLT-1 in the mediation of the signaling mechanisms that modulates neutrophil -endothelial cell cross-talk during inflammation. The basis for the selection of TLT-1 as a potential target emerges from previous studies that demonstrated that TLT-1 knockout mice exhibited increased edema and hemorrhage together with a marked dysfunction of neutrophils during inflammation elicited by the Schwartzman reaction derived vasculitis. Moreover, we have evaluated TLT-1 functions in other models of inflammation including a mouse model of ALI using intranasal inoculation of LPS. Our preliminary data shows that TLT-1 prevents inflammatory associated hemorrhage while facilitate neutrophils transmigration in lung leading to decrease endothelial damage. Therefore, based on these previous observations we hypothesize that TLT-1 mediates the signaling mechanisms that regulate neutrophil capacity to transmigrate to the inflammatory site and modulate neutrophil- endothelial cell interactions during inflammation and therefore controlling the immune response. We developed two specific aims to answer that question. The aims are as follows: (i) Define a role for TLT-1 in platelet-neutrophill-endothelial cell interactions during inflammation and (ii) Elucidate the therapeutic effects of TLT-1 on ALI/ARDS. This two aims will help to fulfill our current understanding of platelet biological functions in inflammation. The answers obtained through the realization of this research work will empower our knowledge to be able to develop new alternative treatments directed to control systemic response to inflammation.

项目总结