Liquid CO2 Flash Chromatography System

应用分离公司建议开发一种价格具有竞争力的液态CO2闪蒸器， 该色谱系统将以高分辨率快速分离化合物， 显著降低有机溶剂消耗。制药企业每年 在药物发现实验室合成数千种化合物， 用于商业化的药物分子。这些合成化合物中的每一种都可能需要 许多反应和纯化步骤用于进一步测试。主要技术用于 制药工业中纯化这些化合物的方法是快速色谱法， 这是因为高样品装载容量和易于使用。目前，Flash 色谱仪器具有差的峰分辨率，并且使用大量的 有机溶剂作为分离中的移动的相。在药物化学实验室中， 快速色谱法是化学废物的主要来源。应用分离开发了 第一阶段原型液体CO2快速色谱系统，成功地使用液体CO2作为 非极性有机溶剂移动的相的替代物。仪器迅速分离 测试化合物具有增加的化合物分辨率、显著的溶剂减少和良好的 复合回收第二阶段建议的具体目标是：（1）重新设计 在对样机进行评价的基础上，对仪器的组成部分进行优化， 可靠性、可维护性和组装性。(2)重新设计软件以提高可用性，包括 计算机接口以及溶剂泵和CO2软件模块的集成。（三） 开发专有的药筒和容器，这些药筒和容器是手动拧紧的， 上级色谱法。(4)最后，构建两个优化的液相快速色谱仪， 在最终设计锁定之前，在生物技术和药物发现实验室进行beta测试。

Applied Separations Inc. proposes to develop a competitively priced liquid CO2 flash chromatography system that will rapidly separate compounds with high resolution and significantly reduce organic solvent consumption. Pharmaceutical companies annually synthesize thousands of compounds in drug discovery laboratories in an attempt to find new drug molecules for commercialization. Each of these synthesized compounds may require many reaction and purification steps for further testing. The primary technique used in the pharmaceutical industry to purify these compounds is flash chromatography and it is preferred because of high sample loading capacity and ease of use. Unfortunately, existing flash chromatography instrumentation suffers from poor peak resolution and uses large volumes of organic solvents as the mobile phase in the separation. Within medicinal chemistry laboratories, flash chromatography is the main source of chemical waste. Applied Separations developed a Phase I prototype liquid CO2 flash chromatography system that successfully used liquid CO2 as a replacement for non polar organic solvent mobile phases. The instrument rapidly separated test compounds with increased compound resolution, significant solvent reduction and good compound recoveries. The specific aims of this Phase II proposal are to: (1) redesign the instrument based on the evaluation of the prototype and optimize the instrument components for reliability, serviceability, and assembly. (2) redesign software to improve usability including the computer interface and the integration of the solvent pump and CO2 software modules. (3) develop proprietary cartridges and vessels that are hand tightened and easy to use with superior chromatography. (4) Finally, construct two optimized liquid flash chromatographs for beta testing at biotechnology and drug discovery laboratories before the final design is locked.