Liquid CO2 Flash Chromatography System

液态CO2快速色谱系统

• 批准号: 9408357
• 负责人: Rolf Schlake
• 金额: $ 49.22万
• 依托单位: APPLIED SEPARATIONS, INC.
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-21 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9408357
• 关键词: Address; Benchmarking; Biotechnology; Carbon Dioxide; Chemicals; Chemistry; Chromatography; Collection; Computer Interface; Computer software; Consumption; Custom; Devices; Drug Industry; Equipment; Evaluation; Feedback; Gases; Hand; Health Services Accessibility; Industry; Laboratories; Laboratory Research; Liquid substance; Manuals; Measures; Organic solvent product; Pharmaceutical Chemistry; Pharmacologic Substance; Phase; Power Sources; Price; Procedures; Process; Pump; Reaction; Recommendation; Recovery; Reporting; Research Contracts; Resolution; Running; Sampling; Scientist; Services; Solvents; Source; System; Techniques; Technology; Temperature; Testing; Time; United States; Universities; Writing; base; commercialization; cost; design; detector; drug discovery; experience; improved; instrument; instrumentation; novel therapeutics; pressure; prototype; quality assurance; repaired; research and development; satisfaction; silochrome; success; usability; wasting

Applied Separations Inc. proposes to develop a competitively priced liquid CO2 flash chromatography system that will rapidly separate compounds with high resolution and significantly reduce organic solvent consumption. Pharmaceutical companies annually synthesize thousands of compounds in drug discovery laboratories in an attempt to find new drug molecules for commercialization. Each of these synthesized compounds may require many reaction and purification steps for further testing. The primary technique used in the pharmaceutical industry to purify these compounds is flash chromatography and it is preferred because of high sample loading capacity and ease of use. Unfortunately, existing flash chromatography instrumentation suffers from poor peak resolution and uses large volumes of organic solvents as the mobile phase in the separation. Within medicinal chemistry laboratories, flash chromatography is the main source of chemical waste. Applied Separations developed a Phase I prototype liquid CO2 flash chromatography system that successfully used liquid CO2 as a replacement for non polar organic solvent mobile phases. The instrument rapidly separated test compounds with increased compound resolution, significant solvent reduction and good compound recoveries. The specific aims of this Phase II proposal are to: (1) redesign the instrument based on the evaluation of the prototype and optimize the instrument components for reliability, serviceability, and assembly. (2) redesign software to improve usability including the computer interface and the integration of the solvent pump and CO2 software modules. (3) develop proprietary cartridges and vessels that are hand tightened and easy to use with superior chromatography. (4) Finally, construct two optimized liquid flash chromatographs for beta testing at biotechnology and drug discovery laboratories before the final design is locked.

Applied Simeations Inc.提议开发竞争价格的液体二氧化碳闪光灯 色谱系统将快速分离具有高分辨率的化合物 大大减少有机溶剂消耗。制药公司每年 合成数千种药物发现实验室中的化合物，以寻找新的化合物 用于商业化的药物分子。这些合成化合物中的每一个都可能需要 许多反应和纯化步骤进行进一步测试。在 纯化这些化合物的制药行业是闪光色谱法，它是首选 由于样品加载能力高和易用性。不幸的是，现有闪光灯 峰值分辨率不佳，并使用大量的峰值分辨率 有机溶剂作为分离的流动阶段。在药物化学实验室中， 闪光色谱法是化学废物的主要来源。应用的分离发展了 I期原型液体CO2闪光色谱系统，成功使用液体CO2作为 替代非极性有机溶剂移动相。仪器迅速分开 测试化合物具有增加的化合物分辨率，显着溶剂降低和良好 复合恢复。该第二阶段提案的具体目的是：（1）重新设计 根据评估原型的仪器，并优化仪器组件 可靠性，可维护性和组装。 （2）重新设计软件以提高可用性 计算机接口以及溶剂泵和二氧化碳软件模块的集成。 （3） 开发专有墨盒和手工收紧且易于使用的容器 上色谱。 （4）最后，为两个优化的液体闪光色谱构造 在最终设计锁定之前，在生物技术和药物发现实验室进行了Beta测试。