Molecular and Neural Mechanisms of Temperature Preference Rhythm in Drosophila

描述（由申请人提供）：生物钟调节生活的许多方面，包括睡眠、活动和体温（BTR）节律。我们最近发现了一种新的果蝇昼夜节律输出，温度偏好节律（TPR），其中苍蝇喜欢在白天温度上升，在夜间温度福尔斯。我们最近发表的数据表明，苍蝇TPR与哺乳动物BTR的共享功能。果蝇是外温动物，通常通过行为调节体温。因此，寻找一个偏好的温度是用来调节苍蝇体温的策略。果蝇脑中的生物钟细胞是哺乳动物视交叉上核神经元的功能同源物。我们发现，一小群昼夜神经元，背神经元2s（DN2s），专门调节TPR，但不运动活动，表明TPR和运动活动是通过不同的昼夜神经元控制。因此，了解TPR将为控制昼夜节律的分子和神经机制提供新的见解。这个提议的目的是确定神经肽如何调节TPR以及热敏神经元如何促进TPR。我们发现，PDF（色素分散因子），一个关键的神经肽的运动活动，是不参与TPR，而神经肽，DH31（利尿激素31），其受体DH31R和PDFR和关键的时钟神经元，DN2s，是必要的正常TPR。在目标1中，我们将阐明DH31调节TPR的机制。在目标2中，我们将研究DN2调节TPR的机制。此外，我们的初步数据表明，热敏神经元是TPR的关键。在目标3中，我们将确定热敏神经元是否参与神经元网络，控制TPR。

DESCRIPTION (provided by applicant): The circadian clock regulates many aspects of life, including sleep and activity and body temperature (BTR) rhythms. We recently identified a novel Drosophila circadian output, temperature preference rhythm (TPR), in which the flies preferred rises in temperature during the day and falls during the night. Our recently published data suggest that fly TPR shares features with mammalian BTR. Drosophila are ectotherms, which typically regulate body temperature behaviorally. Therefore, seeking a preferred temperature is the strategy used to regulate the body temperatures of flies. The circadian clock cells in the fly brain are functional homologs of mammalian SCN (Suprachiasmatic nucleus) neurons. We showed that a small group of circadian neurons, the dorsal neuron 2s (DN2s), specifically regulate TPR, but not locomotor activity, indicating that TPR and locomotor activity are controlled through distinct circadian neurons. Therefore, understanding the TPR will provide new insights into the molecular and neural mechanisms controlling circadian rhythms. The goal of this proposal is to define how neuropeptides regulate TPR and how thermosensory neurons contribute to TPR. We found that PDF (Pigment Dispersing Factor), a critical neuropeptide for locomotor activity, is not involved in TPR, whereas the neuropeptide, DH31 (Diuretic Hormone 31), its receptors DH31R and PDFR and the key clock neurons, DN2s, are necessary for normal TPR. In Aim 1, we will elucidate the mechanisms by which DH31 regulates TPR. In Aim 2, we will examine the mechanisms by which DN2s regulate TPR. Furthermore, our preliminary data suggests that thermosensory neurons are critical for TPR. In Aim 3, we will determine whether thermosensory neurons participate in the neuronal network, controlling TPR.