Infectious Diseases Research Technologies Core - Bethesda

传染病研究技术核心 - Bethesda

• 批准号: 9566796
• 负责人: Robert Hohman
• 金额: $ 1214.28万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9566796
• 关键词: Address; Antibodies; Ascites; Biochemical; Bioinformatics; Biological; Biological Assay; Biomedical Technology; Biophysics; Biotechnology; Cell Separation; Cells; Chemicals; Collaborations; Collection; Color; Communicable Diseases; Communication; Communities; Computer software; Computers; Confocal Microscopy; Consult; Consultations; Containment; Core Facility; Coupling; Crystallization; Custom; DNA; Data; Data Analyses; Data Collection; Data Display; Detection; Development; Discipline; Electron Microscopy; Electrons; Equipment; Experimental Designs; Explosion; Flow Cytometry; Fluorescence; Fluorochrome; Genomics; Goals; High-Throughput Nucleotide Sequencing; Hybridomas; Hypersensitivity; Image; Imaging technology; Immune Sera; Immunology; Industry; Infectious Disease Immunology; Infectious Diseases Research; Institutes; Internet; Intramural Research; Intramural Research Program; Investigation; Label; Laboratories; Lasers; Licensing; Light; Link; Mass Spectrum Analysis; Methods; Microscope; Microscopy; Mission; Molecular Biology; N-terminal; National Institute of Allergy and Infectious Disease; Optics; Peptide Sequence Determination; Peptide Synthesis; Peptides; Preparation; Printing; Protein Analysis; Protein Chemistry; Proteins; Proteomics; Protocols documentation; Publications; RNA; Regimen; Research; Research Activity; Research Infrastructure; Research Personnel; Reservations; Resources; Rest; Roentgen Rays; Running; Sampling; Scientist; Services; Site; Speed; Statistical Data Interpretation; Structure; Surveys; Techniques; Technology; Testing; Time; Training; Training and Education; Transportation; United States National Institutes of Health; Vendor; Video Microscopy; Work; X-Ray Crystallography; animation; assay development; base; biodefense; biophysical analysis; cost; data management; data mining; design; experience; experimental study; genome-wide; image processing; instrument; instrumentation; interest; intravital imaging; light microscopy; macromolecule; meetings; method development; new technology; next generation sequencing; operation; outreach; posters; programs; protein folding; protein structure; reconstruction; research and development; satisfaction; scientific computing; small molecule; structural biology; success; technology development; tool; web app; web site

In 2017, this project provided state-of-the-art research technologies for NIAID's intramural infectious diseases, allergy, and immunology research programs. The new technologies are developed, validated, and then applied in support of NIAID research. Technologies developed outside the NIH are likewise tested, evaluated, validated and, if appropriate, incorporated into the technology portfolio of the NIAID intramural program. The technologies supported include flow cytometry, confocal microscopy, protein separation, mass spectrometry, peptide synthesis, protein sequencing, and macromolecular X-ray crystallography. Many of these technologies are used in high containment laboratories critical to the Institute's infectious diseases and biodefense research agenda. In addition to technology development, the RTB provides advanced training in all aspects of the technologies in the Branch's portfolio. Flow Cytometry The Flow Cytometry Section (FCS) provides the DIR with application-specific flow cytometric technologies, including instrumentation for high speed cell sorting and multi-color analysis. In addition, the FCS provides training, consultation, method development, and analysis for experiments involving flow cytometry. This mission is accomplished through the efforts of staff with extensive flow cytometry experience and with the use of six cell sorters and eight analyzers. Major goals are to provide access to state-of-the art technologies, to help design and run experiments, to facilitate data interpretation and to provide results that are of consistent high quality. FCS consists of a wide variety of instrumentation and provides a cost- and time-efficient method of obtaining fluorescent-labeled custom antibodies. Resources available include hybridoma expansion; antibody purification from ascites, antisera, and hybridoma supernatant; and coupling of purified antibodies and proteins to various fluorochromes. Biological Imaging The Biological Imaging Section (BIS) makes advanced light microscopy imaging technologies and expertise available to DIR scientists. The section collaborates in experimental design, instrument operation and in increasingly-sophisticated image processing routines to best utilize the power of these cutting-edge technologies. Experienced scientists can use the microscopes unattended with staff standing by for troubleshooting, thus allowing the DIR maximal use of facility infrastructure. BIS offers instrumentation and expertise in advanced light microscopy imaging technologies including confocal, fluorescence, video microscopy, and post-collection quantification and deconvolution. BIS is a resource for Stimulated Emission Depletion (STED) microscopy, multiphoton confocal microscopy (intravital imaging), sample preparation, long time-lapse imaging, the NIAID Imaris licenses, and poster printing. Investigators participate in the collection of their data, either as direct microscope operators or by selecting cells of interest, so that the staff can then collect the data. BIS advises on the most suitable instrument, appropriate labels, sample preparation, and best sampling regimens. BIS advises on the correct use of the most appropriate software analysis modules, as well as the best format for displaying the data. Frequently, the Section works with investigators to prepare images for publication including still images, video sequences, 3D reconstructions and time-lapse animations. Protein Chemistry The Protein Chemistry Section develops project specific applications in the fields of peptide synthesis, N-terminal (Edman) sequencing, protein separation, assay development, and mass spectrometry. The RTB designs qualitative and quantitative physical-chemical and biochemical methods of detection and analysis that are customized to meet the specific research needs of DIR investigators. The Branch is also active in formulating and executing purification strategies for various types of molecules. This can also include customized small scale sample preparation and enrichment strategies for analysis by mass spectrometry. Another major activity of the RTB involves the development of more efficient and effective methods of sample preparation with a strong orientation towards mass spectroscopy to facilitate protein identification work. The Branch collaborates with DIR investigators to develop and validate protocols for protein separation and analysis for specific research programs of interest to the intramural research program. The Mass Spectrometry Facility provides support in the planning and execution of mass spectrometry (MS) projects and cooperates with other labs within the RTB to support analysis and characterization of peptides, proteins, and small bio-molecules. The facility offers all facets of mass spectrometry projects including experimental design, separation of biomolecules, sample preparation, data collection and analysis/interpretation of data. The MS facility focuses on analyzing proteins and peptides, and characterizes small molecules upon request. Genomic Technologies The Genomic Technologies Section (GTS) provides state-of-the-art next-generation sequencing, and other massively parallel genomic technologies to intramural investigators. In addition to managing high-end instrumentation, computers, and protocols involved in genomewide assays, the GTS offers statistical analysis and bioinformatics for data interpretation. This support involves close collaboration with the investigators in both experimental design and data analysis to best utilize the power of these applications, as well as to convey a better understanding of the technology. GTS develops project-specific research applications for many analysis platforms at the DNA, RNA and protein level. Statistical analysis, data management, and data mining solutions are available as well, including from experimental concept to publication and public data submission. The Section maintains instrumentation and resources for Agilent, Illumina, and other microarray platforms, Illumina (Solexa) high throughput sequencing, commercial and custom bioinformatics software, and scientific computing. Structural Biology The Structural Biology Section (SBS) provides specialized techniques and scientific expertise that enable DIR scientists to obtain biophysical and structural data for macromolecules. While closely collaborating with DIR researchers, the SBS provides consulting/training, produces pure proteins, performs biophysical analyses, and determines structures of proteins and other macromolecules that are central to the infectious disease and immunology research programs of the DIR. Biophysical analysis and structural biology require expertise in producing large amounts of correctly folded proteins at high-purity, in preparing diffraction-quality crystals, and in determining crystal structures by X-ray methods. With an emphasis on providing training in biochemical, biophysical, and structural methods, the SBS makes it possible for investigators to use structurally based ideas and techniques in their research program.

2017年，该项目为NIAID的壁内传染病，过敏和免疫学研究计划提供了最先进的研究技术。新技术得到了开发，验证，然后应用于支持NIAID研究。同样对NIH之外开发的技术进行了测试，评估，验证，并在适当的情况下纳入NIAID壁内计划的技术组合中。 支持的技术包括流式细胞术，共聚焦显微镜，蛋白质分离，质谱法，肽合成，蛋白质测序和大分子X射线晶体学。 这些技术中的许多技术用于对该研究所的传染病和生物形式研究议程至关重要的高遏制实验室。 除技术开发外，RTB还提供了分支机构投资组合中技术各个方面的高级培训。 流式细胞仪 流式细胞仪部分（FCS）为DIR提供了特定的流式细胞仪技术，包括用于高速细胞分类和多色分析的仪器。此外，FCS为涉及流式细胞仪的实验提供培训，咨询，方法开发和分析。通过具有丰富的流式细胞仪经验以及使用六个细胞分类器和八个分析仪的员工的努力来完成此任务。主要目标是提供最先进的技术，以帮助设计和运行实验，促进数据解释并提供一致的高质量结果。 FC由多种仪器组成，并提供了一种成本和耗时的方法来获得荧光标记的自定义抗体。可用的资源包括杂交瘤扩展；腹水，抗血清和杂交瘤上清液中的抗体纯化；以及纯化的抗体和蛋白质与各种荧光染料的偶联。 生物成像 生物成像部分（BIS）使高级显微镜成像技术和专业知识可供DIR科学家使用。该部分在实验设计，仪器操作以及越来越熟练的图像处理程序方面合作，以最佳利用这些尖端技术的力量。经验丰富的科学家可以将无人看管的显微镜与人员站在一起进行故障排除，从而使设施基础设施的最大使用。 BIS提供了高级光学显微镜成像技术的仪器和专业知识，包括共聚焦，荧光，视频显微镜以及收集后定量和反向卷积。 BIS是刺激发射耗竭（STED）显微镜，多光子共聚焦显微镜（插入式成像），样品制备，长时间成像，NIAID IMARIS许可证和海报打印的资源。调查人员可以作为直接显微镜操作员或选择感兴趣的单元格进行数据收集，以便员工可以收集数据。 BIS建议最合适的仪器，适当的标签，样品制备和最佳采样方案。 BIS建议正确使用最合适的软件分析模块，以及显示数据的最佳格式。本节通常与研究人员合作，准备出版图像，包括静止图像，视频序列，3D重建和延时动画。 蛋白质化学 蛋白质化学部分在肽合成，N末端（EDMAN）测序，蛋白质分离，测定开发和质谱法中开发了项目的特定应用。 RTB设计定性和定量的物理化学和生化方法的检测和分析方法，这些方法可定制，以满足DIR研究者的特定研究需求。该分支还积极地为各种类型的分子制定和执行纯化策略。这还可以包括定制的小型样品制备和质谱分析的富集策略。 RTB的另一个主要活性涉及开发更有效的样品制备方法，并具有强烈​​的质谱方向，以促进蛋白质鉴定工作。 该分支机构与DIR调查人员合作开发和验证方案，以针对壁内研究计划的特定研究计划进行蛋白质分离和分析。 质谱设施为质谱（MS）项目的计划和执行提供了支持，并与RTB中的其他实验室合作，以支持肽，蛋白质和小型生物分子的分析和表征。 该设施提供了质谱项目的所有方面，包括实验设计，生物分子的分离，样品制备，数据收集和数据分析/解释。 MS设施的重点是分析蛋白质和肽，并应要求表征小分子。 基因组技术 基因组技术科（GTS）提供了最先进的下一代测序，并为室内研究者提供了其他大规模平行的基因组技术。除了管理全基因组测定法所涉及的高端仪器，计算机和协议外，GTS还提供统计分析和生物信息学用于数据解释。这种支持涉及与研究人员在实验设计和数据分析方面的密切合作，以最好地利用这些应用程序的力量，并传达对技术的更好理解。 GTS为DNA，RNA和蛋白质水平的许多分析平台开发了项目特异性研究应用。还提供统计分析，数据管理和数据挖掘解决方案，包括从实验概念到出版物和公共数据提交。 本节为Agilent，Illumina和其他微阵列平台维护仪器和资源，Illumina（Solexa）高通量测序，商业和自定义生物信息学软件以及科学计算。 结构生物学 结构生物学部分（SB）提供了专业的技术和科学专业知识，使DIR科学家能够获得大分子的生物物理和结构数据。在与DIR研究人员密切合作的同时，SBS提供咨询/培训，产生纯蛋白质，进行生物物理分析，并确定蛋白质和其他大分子的结构，这些结构是DIR的感染性疾病和免疫学研究计划的核心。生物物理分析和结构生物学需要在高纯度，制备衍射质量晶体以及通过X射线方法确定晶体结构时，在高纯度时生产大量正确折叠的蛋白质方面需要专业知识。在重点是提供生化，生物物理和结构方法的培训，SBS使研究人员可以在其研究计划中使用基于结构的思想和技术。