Infectious Diseases Research Technologies Core - Bethesda

传染病研究技术核心 - Bethesda

• 批准号: 7732699
• 负责人: Robert Hohman
• 金额: $ 1159.03万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7732699
• 关键词: Address; Allergy and Immunology; Amino Acid Sequence; Arts; Biochemical; Bioinformatics; Cell Separation; Chemicals; Collaborations; Collection; Communicable Diseases; Confocal Microscopy; Containment; Custom; DNA Microarray Chip; DNA Microarray format; Data; Data Analyses; Detection; Development; Environment; Flow Cytometry; Fluorescence; Genome; Genomics; Goals; Infectious Diseases Research; Institutes; Intramural Research Program; Label; Laboratories; Mass Spectrum Analysis; Measures; Methods; N-terminal; National Institute of Allergy and Infectious Disease; Oligonucleotide Microarrays; Peptide Sequence Determination; Peptide Synthesis; Preparation; Protein Chemistry; Proteins; Proteomics; Protocols documentation; Research; Research Personnel; Research Project Grants; Sampling; Scientist; Solutions; System; Technology; Testing; Training; Transcript; United States National Institutes of Health; Video Microscopy; Work; assay development; base; biodefense; data management; data mining; design; interest; light microscopy; new technology; nucleic acid purification; programs; response; technology development

This project provides state-of-the-art research technologies for NIAID's intramural infectious diseases, allergy, and immunology research programs. The new technologies are developed and validated and then applied in support of NIAID research. Technologies developed outside the NIH are likewise tested, evaluated, validated and, if appropriate, incorporated into the technology portfolio of the NIAID intramural program. The technologies supported include flow cytometry, confocal microscopy, DNA microarray, protein separation, mass spectrometry, peptide synthesis and protein sequencing. Many of these technologies are used in high containment laboratories critical to the Institute's infectious diseases and biodefense research agenda. In addition to technology development, the RTB provides advanced training in all aspects of the technologies in the Branchs portfolio. Flow Cytometry Specific research applications are developed for flow cytometry analysis and cell sorting in BSL-2 and BSL-3 environments. Light Microscopy The RTB develops applications in many different fields of light microscopy including confocal, fluorescence, and video microscopy, as well as post-collection quantification and deconvolution. The RTB has expertise in developing specific applications for a high containment (BSL-3) setting. Protein Chemistry The Protein Chemistry Section develops applications in the fields of peptide synthesis, N-terminal (Edman) sequencing, protein separation, assay development, and mass spectrometry. The RTB designs qualitative and quantitative physical-chemical and biochemical methods of detection and analysis that are customized to meet the specific research needs of DIR investigators. The Branch is also active in formulating and executing purification strategies for various types of molecules. This can also include customized small scale sample preparation and enrichment strategies for analysis by mass spectrometry. Another major activity of the RTB involves the development of more efficient and effective methods of sample preparation with a strong orientation towards mass spectroscopy to facilitate protein identification work. The Branch collaborates with DIR investigators to develop and validate protocols for protein separation and analysis for specific research programs of interest to the intramural research program. Microarray and Bioinformatics The RTB develops specific solutions for microarray-based research project for the NIAID investigators. The Branch designs custom oligonucleotide microarrays for investigators who plan to measure the response profiles of transcripts at the genome level. RTB scientists develop new protocols for nucleic acid purification, labeling, hybridization and data analysis. In addition, the Branch trains DIR investigators on all aspects of the technology. In addition to developing new applications for microarray research, the RTB develops statistical analysis, data management, and data mining solutions for DIR research programs; focusing on interpreting data generated by highly parallel detection systems used in genomics and proteomics.

该项目为NIAID的壁内传染病，过敏和免疫学研究计划提供了最先进的研究技术。新技术经过开发和验证，然后应用于支持NIAID研究。同样对NIH之外开发的技术进行了测试，评估，验证，并在适当的情况下纳入NIAID壁内计划的技术组合中。 支持的技术包括流式细胞术，共聚焦显微镜，DNA微阵列，蛋白质分离，质谱，肽合成和蛋白质测序。 这些技术中的许多技术用于对该研究所的传染病和生物形式研究议程至关重要的高遏制实验室。 除技术开发外，RTB还提供了分支机构投资组合中各个方面的高级培训。 流式细胞仪 为BSL-2和BSL-3环境中的流式细胞仪分析和细胞分类开发了特定的研究应用。 光学显微镜 RTB在许多不同领域的光学显微镜中开发了应用，包括共焦，荧光和视频显微镜以及收集后定量和反向卷积。 RTB在开发高遏制（BSL-3）设置的特定应用程序方面具有专业知识。 蛋白质化学 蛋白质化学部分在肽合成，N末端（EDMAN）测序，蛋白质分离，测定开发和质谱法中开发了应用。 RTB设计定性和定量的物理化学和生化方法的检测和分析方法，这些方法可定制，以满足DIR研究者的特定研究需求。该分支还积极地为各种类型的分子制定和执行纯化策略。这还可以包括定制的小型样品制备和质谱分析的富集策略。 RTB的另一个主要活性涉及开发更有效的样品制备方法，并具有强烈​​的质谱方向，以促进蛋白质鉴定工作。 该分支机构与DIR调查人员合作开发和验证方案，以针对壁内研究计划的特定研究计划进行蛋白质分离和分析。 微阵列和生物信息学 RTB为NIAID研究人员开发了基于微阵列的研究项目的特定解决方案。该分支设计了计划在基因组水平上测量转录本的响应曲线的研究人员的自定义寡核苷酸微阵列。 RTB科学家开发了用于核酸纯化，标记，杂交和数据分析的新方案。 此外，分支机构还培训DIR调查人员有关该技术的各个方面。 除了为微阵列研究开发新的应用程序外，RTB还开发了DIR研究计划的统计分析，数据管理和数据挖掘解决方案；重点是解释由基因组学和蛋白质组学中使用的高度平行检测系统产生的数据。