Extreme High Throughput Screening Via Massively Parallel Droplet Dispenser Arrays

项目摘要 该项目旨在通过使筛选技术更加有效， 容易接近目前，新的药物线索是通过测试大量未知化学物质发现的 看看是否有你想要的效果。由于这个过程有点慢， 这种测试费用昂贵，但并不像它应该的那样彻底和广泛。我们试图让 化学反应将在微小的液滴中进行，这将大大降低生产成本。 这些反应，使它有可能运行更多的一起一次。 为了做到这一点，我们正在建造一个新的设备，一个巨大的液滴分配器阵列。阵列 设想它足够大，可以容纳你感兴趣的整套未知化学物质， 试验.数组是非常灵活的。它可以快速分配整套化学品， 每种化学品的不同浓度，以便我们可以找到最有效的剂量，或分配 化学物质的各种不同组合，这样我们就可以发现哪些药物可以很好地配合使用。 为了使这一系列的分配器工作，我们正在使用一种新型的微芯片技术 其中电路携带液体而不是电子。我们的液体回路的独特之处在于 可以像计算机一样进行计算。这最终对这个项目非常有用 因为我们使用这种计算能力来控制大量的分配器。 一旦我们建立了液滴分配器，我们将测试他们在各种类型的共同筛选 实验我们将尝试测试一个化学品库对一种酶，认为是重要的 癌症的扩散作为测试的一部分，我们将尝试各种不同的变化， 每种化学品的浓度，以确定它们开始生效的剂量。另外我们 将开发另一个系统，这样我们就可以直接在结肠癌细胞上测试我们未知的化学物质， 看看是否有化学物质会杀死细胞或减缓它们的生长。在这些测试中，我们将 该设备的能力，使不同的化学品和药物的组合优势。通常，癌症 细胞需要被多种药物击中才能产生积极的效果。测试不同的药物 这些癌细胞上的组合将帮助我们发现有效的联合收割机药物的新方法。 这项工作不是关于药物测试本身，而是关于开发新的测试方法 可能的药物。一旦我们能够证明我们的方法有效，我们就可以分享我们的方法， 帮助其他人更快地发现新的治疗方法。

PROJECT SUMMARY This project seeks to make drug discovery more effective by making the screening technology more accessible. Currently, new drug leads are discovered by testing a large number of unknown chemicals to see if any have the effect that you are looking for. Since this process is somewhat slow and expensive, such testing is not as thorough and widespread as it should be. We are trying to allow the chemical reactions to be run in microscopic droplets of liquid, which would greatly lower the cost of these reactions and make it possible to run many more together at once. To accomplish this, we are building a new device, a gigantic array of droplet dispensers. The array is envisioned to be large enough to hold the entire set of unknown chemicals that you are interested in testing. The array is very flexible. It can quickly dispense the entire set of chemicals, dispense many different concentrations of each chemical so that we can find the most effective dose, or dispense the chemicals in a variety of different combinations so that we can discover which drugs work well together. In order to make this array of dispensers work, we are using a new type of microchip technology where the circuits carry liquids instead of electrons. What is unique about our liquid circuits is that they can perform computations much like a computer can. This ends up being very useful for this project because we use this computational power to control the large array of dispensers. Once we build the droplet dispensers, we will test them out in various types of common screening experiments. We will try testing a library of chemicals against an enzyme that thought to be important in the spread of cancer. As part of this testing, we will try the variation where we try many different concentrations of each chemical, to find the dosage at which they start to become effective. Also, we will develop another system so that we can test our unknown chemicals directly on colon cancer cells, to see if any chemicals will kill the cells or slow down their growth. During these tests, we will take advantage of the device's ability to make different combinations of chemicals and drugs. Often, cancer cells need to be hit with multiple drugs in order to have a positive effect. Testing different drug combinations on these cancer cells will help us to discover new ways to combine drugs effectively. This work is less about the drug testing itself, but rather it's more about developing new ways to test possible drugs. Once we can show that our methods work, we can share our methods and hopefully help others discover new cures more quickly.