Elucidating the role of SUMO ligase Su(var)2-10 in piRNA-guided transcriptional silencing and repressive chromatin formation

阐明 SUMO 连接酶 Su(var)2-10 在 piRNA 引导的转录沉默和抑制染色质形成中的作用

• 批准号: 9806312
• 负责人: Maria Antoninova NINOVA
• 金额: $ 12.95万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9806312
• 关键词: Address; Affect; Aging; Animals; Architecture; Biochemical; Biochemical Genetics; Biological Assay; Cell physiology; Cells; Chromatin; Chromatin Remodeling Factor; Chromosomal Instability; Chromosomes; Complex; Coupled; DNA; DNA Damage; DNA Transposable Elements; DNA-Binding Proteins; Data; Defect; Deposition; Development; Disease; Drosophila genus; Embryo; Embryonic Development; Ensure; Environment; Epigenetic Process; Eukaryota; Event; Feedback; Female; Fertility; Future; Gametogenesis; Gene Expression; Gene Silencing; Genes; Genetic; Genetic Recombination; Genetic Transcription; Genome; Genomic Segment; Genomics; Germ Cells; Goals; Guide RNA; Heterochromatin; Histone H3; Histones; Homeostasis; Human; Image; In Vitro; Knowledge; Lead; Ligase; Lysine; Maintenance; Malignant Neoplasms; Methods; Modeling; Modification; Molecular; Monitor; Normal Cell; Ovary; Pathway interactions; Phase; Play; Post-Translational Protein Processing; Process; Proteins; Proteomics; RNA Interference; Reagent; Regulation; Repetitive Sequence; Reporter; Repression; Research Personnel; Research Training; Role; Small RNA; Sterility; Structure; System; Testing; Work; Yeasts; arm; discrete time; epigenetic regulation; genetic approach; genetic element; genome-wide; histone methyltransferase; histone modification; in vivo; insight; novel; piRNA; recruit; research facility; sensor; time interval; ubiquitin-protein ligase

Project Summary: Heterochromatin refers to the compacted and transcriptionally suppressed chromatin state that typically includes repeat-rich regions near chromosomal arm ends, transposable elements (TEs), as well as some genes. Heterochromatin plays important architectural and regulatory roles, and its misregulation leads to aberrant gene expression and chromosome instability associated with cancers, aging and in germ cells, embryonic lethality and sterility. Large fraction of heterochromatin from yeast to humans is marked by histone H3 lysine 9 trimethylation (H3K9me3). H3K9me3 is deposited by histone mark “writer” complexes which can be recruited to genomic targets by DNA binding proteins or small RNA guides. Many aspects of heterochromatin establishment and maintenance in the cell and in development remain poorly understood. Heterochromatin regulation is the central focus of this proposal. In germ cells, Piwi proteins and associated Piwi-interacting small RNAs (piRNAs) guide a writer complex to install the H3K9me3 mark and induce transcriptional silencing at TE targets. TE repression by piRNAs is essential for animal fertility, yet its mechanism is not known. Candidate's previous work showed that localization to chromatin of the conserved SUMO E3 ligase Su(var)2- 10 induces heterochromatin formation in germ cells of the Drosophila ovary. Data led to a model that Su(var)2- 10 forms a complex with piRNA-Piwi at genomic targets, and deposits SUMO at yet-to-be-established factor(s), which in turn recruits the H3K9me3 writer dSetDB1. Su(var)2-10 also controls H3K9me3 deposition at piRNA- independent loci, including genes of several silencing factors, indicating a novel negative feedback mechanism between heterochromatin levels and silencing factors that can explain how germ cells maintain heterochromatin levels to ensure proper genome function. This proposal presents a strategy to elucidate the role of Su(var)2-10/SUMO in piRNA-guided silencing, and to investigate the auto-regulation and developmental inheritance of Su(var)2-10 dependent heterochromatin. The candidate will characterize the substrates of SUMO modification by Su(var)2-10 using state-of-the-art proteomics coupled with RNAi (Aim 1), and use biochemical and genetic approaches to investigate the mechanisms that lead to Su(var)2-10 localization and SUMO-dependent dSetDB1 recruitment to genomic targets (Aim 2). In the long term, the candidate will investigate the proposed model of heterochromatin regulation by negative feedback, and study the stability of repressed chromatin states induced by Piwi and Su(var)2-10 across development (Aim 3). Together, this project will provide deep mechanistic insight into heterochromatin formation in germ cells, and address fundamental principles of epigenetic regulation relevant to normal cell function and disease states. Aim 1 and 2 will be initiated during the K99 phase in Dr. Alexei Aravin's lab at Caltech. This environment will provide all necessary research facilities and training to achieve the proposed goals, and to generate reagents and data for future studies, allowing a smooth transition to an independent researcher phase (Aim 3/R00).

项目概述：异染色质是指染色质紧致和转录抑制的状态