Light Triggered Controls of Mosquito Diapause

项目摘要 昆虫利用多种发育和生理手段来限制 恶劣的气候和环境。温带地区许多昆虫使用的一个关键策略是 环境，包括重要的蚊子媒介物种，是季节性滞育。这 行为的特点是整个冬季的发育停滞，通常由以下因素引发： 光周期启动滞育准备。昆虫一般是如何 蚊子媒介专门测量这种光周期来启动这种行为， 探索不足该项目旨在描述滞育的遗传基础 白纹伊蚊（Aedes albopictus）的光感行为， 光周期性我们试图具体了解视觉系统的组成部分是什么 与日长的测量有关。 最近由CRISPR-Cas9实现的基因编辑技术的扩展- 介导的转基因形成了该提议的重点。我们将生产各种 实验室维持蚊系在光检测的不同方面缺乏测定 白纹伊蚊的能力，准备，进入和退出滞育时，饲养 适当的象征性刺激。此外，这项建议还需要进一步了解 这种蚊子的视觉生理学，特别是根据我们最近的工作， 展示了埃及伊蚊和井蚊之间的视觉系统差异， 研究果蝇。这一建议将解决以前被忽视的领域。 利用现代技术进行滞育研究，并将为今后的工作奠定基础 无论是在滞育还是在蚊子的视觉系统上。 基因在发育和生活史中的作用 决策显示出为蚊子控制和基因驱动产生新目标的希望 技术.这一努力可能会确定发展目标控制的可能性 战略布局

PROJECT SUMMARY Insects use of multiple developmental and physiological means to limit the impact of adverse climate and environments. One key strategy used by many insects in temperate environments, including important mosquito vector species, is seasonal diapause. This behavior is marked by developmental arrest throughout the winter period, often triggered by photoperiod to initiate diapause preparation. The mechanics of how insects in general and mosquito vectors specifically measure this photoperiod to initiate this behavior remains underexplored. This project seeks to characterize the genetic underpinnings of diapause behavior in the mosquito species Aedes albopictus induced by light sensation and photoperiodicity. We seek to understand specifically what elements of the visual system are implicated in the measurement of day length. The recent expansion in gene editing technologies enabled by CRISPR-Cas9- mediated transgenesis form the keystone of this proposal. We will produce a variety of laboratory-maintained mosquito lines deficient in distinct aspects of light detection to assay Aedes albopictus’s ability to prepare for, enter, and exit diapause when reared with appropriate token stimuli. Additionally, this proposal requires increased understanding of visual physiology of this mosquito species, particularly in light of our recent work demonstrating visual system differences between the mosquito Aedes aegypti and the well- studied Drosophila melanogaster. This proposal will address areas neglected in previous diapause research utilizing modern technologies and will set groundwork for future work both in diapause and generally on the mosquito visual system. The identification of the roles of genes involved in developmental and life history decisions show promise for producing new targets for mosquito control and gene drive technologies. This effort may identify possibilities for development-targeting control strategies.