Light Triggered Controls of Mosquito Diapause

光触发控制蚊子滞育

• 批准号: 9808732
• 负责人: JOSEPH E O'TOUSA
• 金额: $ 23.18万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-05 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9808732
• 关键词: Address; Adult; Aedes; Animals; Arboviruses; Area; Arthropods; Behavior; Biological Assay; Brain; CRISPR/Cas technology; Cells; Climate; Competence; Culicidae; Dengue; Detection; Development; Diapause; Disease; Drosophila genus; Drosophila melanogaster; Elements; Embryo; Embryonic Development; Environment; Esthesia; Exposure to; Female; Future; Gene Transfer Techniques; Genes; Genetic; Genetic Crosses; Germ Lines; Goals; Health; Hibernation; Human; Insecta; Invaded; Laboratories; Light; Measurement; Measures; Mechanics; Mediating; Modernization; Monitor; Mosquito Control; Mosquito-borne infectious disease; Ocular Physiology; Orphan; Orthologous Gene; Phospholipase C; Photoperiod; Phototransduction; Physiological; Population; Preparation; Process; Production; Proteins; Research; Retinal; Retinal Photoreceptors; Rhodopsin; Risk; Role; Seasons; Signal Transduction; Stimulus; System; Technology; Tropical Disease; United States; Visual system structure; Work; ZIKV infection; Zika Virus; base; chikungunya; circadian; circadian pacemaker; cryptochrome 1; day length; falls; gene drive system; gene function; genetic strain; life history; mutant; neglect; novel; phospholipase C beta; photoperiodicity; receptor; sensor; synergism; vector; vector mosquito

PROJECT SUMMARY Insects use of multiple developmental and physiological means to limit the impact of adverse climate and environments. One key strategy used by many insects in temperate environments, including important mosquito vector species, is seasonal diapause. This behavior is marked by developmental arrest throughout the winter period, often triggered by photoperiod to initiate diapause preparation. The mechanics of how insects in general and mosquito vectors specifically measure this photoperiod to initiate this behavior remains underexplored. This project seeks to characterize the genetic underpinnings of diapause behavior in the mosquito species Aedes albopictus induced by light sensation and photoperiodicity. We seek to understand specifically what elements of the visual system are implicated in the measurement of day length. The recent expansion in gene editing technologies enabled by CRISPR-Cas9- mediated transgenesis form the keystone of this proposal. We will produce a variety of laboratory-maintained mosquito lines deficient in distinct aspects of light detection to assay Aedes albopictus’s ability to prepare for, enter, and exit diapause when reared with appropriate token stimuli. Additionally, this proposal requires increased understanding of visual physiology of this mosquito species, particularly in light of our recent work demonstrating visual system differences between the mosquito Aedes aegypti and the well- studied Drosophila melanogaster. This proposal will address areas neglected in previous diapause research utilizing modern technologies and will set groundwork for future work both in diapause and generally on the mosquito visual system. The identification of the roles of genes involved in developmental and life history decisions show promise for producing new targets for mosquito control and gene drive technologies. This effort may identify possibilities for development-targeting control strategies.

项目总结