Mutagenesis due to Translesion Polymerase Usage during Replication and Repair

复制和修复过程中使用跨损伤聚合酶引起的突变

• 批准号: 9269234
• 负责人: Mitch McVey
• 金额: $ 28.7万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9269234
• 关键词: Affect; Appearance; Attention; Chromatin; Chromosomal Breaks; Collaborations; Complex; DNA Repair; DNA-Directed DNA Polymerase; Disease; Double Strand Break Repair; Drosophila genus; Event; Frequencies; Galactose; Gene Conversion; Gene Targeting; Genetic Recombination; Goals; HO nuclease; Health; Human; Light; Mediating; Mutagenesis; Mutation; Polymerase; Proteins; Role; Saccharomycetales; Single-Stranded DNA; Site; Work; genetic analysis; helicase; homologous recombination; human disease; member; prevent; programs; repaired

This proposal focuses on several mutational changes accompanying DNA repair in budding yeast. DNA repair is induced by galactose-regulated expression of the site-specific HO endonuclease, creating a single double-strand break (DSB). One major goal is to understand complex mutations associated by template switching that occur during gene conversion. Two types of repair will be studied: 1. Quasipalindrome mutation formation during gene conversion and 2. Interchromosomal microhomology-mediated template switching during gene conversion. Genetic analysis of helicases and other repair factors will be screened to find proteins that control the level of these two events, along with an exploration of the role of chromatin. A second goal is to understand changes in repeat copy number during gene conversion, motivated by our recent discovery of important differences between DSB break repair and gap repair. In collaboration with Mitch McVey, another member of this Program Project who focuses on DSB repair in fruit flies, we will assess the frequency of abortive gap repair leading to deletions between repeated sequences within the copied region.

这一建议集中在几个突变变化伴随DNA修复出芽酵母。DNA