National Resource for Translational and Developmental Proteomics

 描述（由申请人提供）：在这里，我们提出了一个国家资源的翻译和发育蛋白质组学（NRTDP）。科学驱动因素在八个驱动生物医学项目中概述，这些项目激发了四个技术研究和开发项目的具体目标。这些联合活动的影响，致力于改善质谱为基础的蛋白质组学使用整个蛋白质作为测量的主要单位，将是巨大的。综合资源的各个方面（包括生物医学应用，技术开发，传播和培训），预计的影响与P41生物医学技术研究资源的精神和文字以及NIH的战略目标保持一致。将创新应用于临床和转化研究各个领域的及时挑战，将确保我们国家在“下一代”蛋白质组学技术中的领导地位。这些技术将有助于推动项目和研究人员提高科学理解和诊断，最终改善癌症患者、肾移植接受者、冠心病患者的生活，并保护癌症女性幸存者的生育能力。更具体地说，我们将把超高性能蛋白质组学集中在来自各种癌症研究的样本集上，包括慢性淋巴细胞白血病，多发性骨髓瘤和急性髓性白血病。在技术方面，我们将加速开发自上而下的蛋白质组学领先平台，包括两种新的傅立叶变换仪器、新的蛋白质分离技术和AUTOPILOT软件套件，以实现生物医学项目的战略目标，管理日常数据收集的最详细方面。一个量身定制的工作流程，使核心组蛋白的普遍和详尽的分析，进一步扩大了技术组合，可在这个深度合作和恭敬的国家资源住房明显高性能的技术和行政团队。该团队有能力将高端研究活动提炼成强大的服务产品线，这一点已经得到了很好的证明。 对实验室作为培训中心的兴趣一直很强烈（迄今已有十多名访问学者），并包括扩大这些活动的具体计划。申请中还描述了信息和技术传播的几条途径。所有这些活动的管理和执行是由一个久经考验的高级团队在西北部的蛋白质组学卓越中心（PCE），这也有非常强大的机构支持，以调整蛋白质组学正在芝加哥与NIH资助的生物和转化研究的国家优先事项。这里设想的普通医学科学的进步将引起包括NCI、NICHD、NIDDK、NIGMS和NHLBI在内的几个研究所的兴趣，所有这些研究所都在蛋白质组学研究方面投入了大量资金。我们在蛋白质组学卓越中心的高级研究人员团队，沿着与全国十几个合作实验室一起，期待着关于拟议的NRTDP的反馈。

 DESCRIPTION (PROVIDED BY APPLICANT): Here we propose a National Resouce for Translational and Developmental Proteomics (NRTDP). The science drivers are outlined in eight Driving Biomedical Projects that motivate the specific aims for four Technology Research and Development Projects. The impact of these combined activities, devoted to improving mass spectrometry-based proteomics using whole proteins as the primary unit of measurement, will be substantial. Integrated over all aspects of the resource (including biomedical application, technology development, dissemination and training), the projected impacts are aligned with both the spirit and the letter of a P41 Biomedical Technology Research Resource and the strategic goals of the NIH as a whole. Applying innovation to timely challenges in diverse areas of clinical and translational research will secure a leadership position for our nation in 'next-generation' proteomics technology. These technologies will serve driving projects and investigators to improve scientific understanding and diagnostics, ultimately improving the lives of cancer patients, recipients of kidney transplants, those afflicted with coronary heart disease, and to preserve fertility in women survivors of cancer (oncofertility). More specifically, we will focus ultra-high performance proteomics on sample sets deriving from research on various cancers, including chronic lymphocytic leukemia, multiple myeloma, and acute myeloid leukemia. In technology, we will accelerate the development of the leading platform for top-down proteomics, including two new Fourier-Transform instruments, new protein fractionation technologies, and the AUTOPILOT software suite to allow strategic objectives of biomedical projects to govern the most detailed aspects of data collection on a daily basis. A tailored workflow to enable pervasive and exhaustive analysis of core histones further augments the technology portfolio available in this deeply collaborative and deferential national resource housing a demonstrably high performing technical and administrative team. Good evidence of the team's ability to distill high-end research activities into robust servce lines is now visible. Interest in the laboratory as a hub for training has been robust (with over a dozen visiting scholars to date) and specific plans for expansion of these activities are included. Several routes of information and technology dissemeniation also described in the application. The administration and execution of all these activities is assured by a proven senior team operating the Proteomics Center of Excellence (PCE) at Northwestern, which also has very strong institutional backing to align proteomics ongoing in Chicago with national priorities of NIH-funded biological and translational research of the NIH. The advancement of general medical science envisioned here will be of interest to several institutes including NCI, NICHD, NIDDK, NIGMS, and NHLBI - all of which are heavily invested in proteomics research. Our team of senior researchers at the Proteomics Center of Excellence, along with over a dozen collaborating laboratories around the country, look forward to feedback regarding the proposed NRTDP.