Purchase of an imaging flow cytometer 2016

2016年购买成像流式细胞仪

• 批准号: 9273704
• 负责人: Rick L Tarleton
• 金额: $ 45.38万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-15 至 2018-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9273704
• 关键词: Adipose tissue; Affect; African Trypanosomiasis; Agriculture; Biotechnology; Cattle; Cell Cycle; Cell Cycle Progression; Cell Nucleus; Cell physiology; Cells; Chagas Disease; Communicable Diseases; Communities; Cytometry; Detection; Disease; Drug resistance; Environmental Impact; Environmental Pollutants; Flow Cytometry; Fluorescence; Funding; Genes; Image; Immunology; Infection; Infectious Diseases Research; Institution; Investigation; Knowledge; Laboratories; Latin America; Malaria; Microscopy; Obesity; Pluripotent Stem Cells; Request for Proposals; Research; Resource Sharing; Speed; Stem cells; Universities; Viral; Virus Replication; cancer therapy; drug development; high resolution imaging; insight; instrument; nagana; programs; stem cell differentiation

Project Summary/Abstract: This proposal requests funds to purchase an ImageStream Mark II imaging flow cytometer for the Cytometry Shared Resource Laboratory (CSRL) at the University of Georgia. The ImageStream combines the speed, sensitivity, and fluorescence detection capabilities of flow cytometry with the detailed imaging and functional insights of microscopy. This instrument produces multiple, high- resolution images of every cell directly in flow, including brightfield and darkfield and up to 10 fluorescent markers. The CTEGD Cytometry SRL is the central flow cytometry facility for UGA and is housed within and staffed by the UGA Center for Tropical and Emerging Global Diseases (CTEGD). For more than 15 years the CTEGD CSRL has served the entire university community and surrounding biotechnology companies as well as regional academic institutions. Major users of the facility include laboratories with research foci in infectious diseases, stem cell differentiation, environmental impacts on cellular functions and obesity. Among infectious disease research programs to be supported by this purchase are investigations of Chagas disease, a disease that affects >20 million people in Latin America and the U.S., African sleeping sickness and Nagana, an infection of cattle that results in ~$4 billion in agricultural loss per year, and malaria, one of the “big 3” infectious disease killers. Viral immunology is the focus of 2 of our major users who are studying the interaction between host genes and viral replication as it relates to drug resistance. The ImageStream will allow stem cell and cellular biologists to better understand the cell cycle progression of pluripotent stem cells and the impact of new cancer therapies, as well as to correlate the cell cycle with the effects of environmental pollutants. The knowledge obtained by studying adipose nuclei is projected to facilitate drug development in the field of obesity. Thus, we expect the ImageStream to have a broad impact on the diverse research across the UGA campus.

项目摘要/摘要： 该提案要求资金购买成像标记II成像流量 细胞仪的胞胎仪共享资源实验室（CSRL） 佐治亚大学。成像级结合了速度，灵敏度，并且 流式细胞术的荧光检测能力，具有详细成像 显微镜的功能见解。该仪器产生多个高级 直接流中每个单元的分辨率图像，包括Brightfield和Darkfield 和多达10个荧光标记。 CTEGD细胞仪SRL是中心流 UGA的细胞仪设施，由UGA中心内部和人员组成 用于热带和新兴的全球疾病（CTEGD）。超过15年 CTEGD CSRL为整个大学社区和周边地区提供了服务 生物技术公司以及区域学术机构。主要用户 该设施包括具有研究焦点的实验室，用于传染病，STEM 细胞分化，环境对细胞功能和肥胖的影响。 在此购买的传染病研究计划中 是对查加斯病的调查，这种疾病影响了> 2000万人 拉丁美洲和美国，非洲昏睡病和纳迦纳，一种感染 牛每年造成约40亿美元的农业损失，而疟疾是 “ 3大”传染病杀手。病毒免疫学是我们2的重点 研究宿主基因与病毒之间相互作用的主要用户 与耐药性有关的复制。成像级将允许干细胞 和细胞生物学家，以更好地了解细胞周期的进展 多能干细胞以及新的癌症疗法的影响，以及 将细胞周期与环境污染物的影响相关。 通过研究脂肪核获得的知识预计是为了促进药物 肥胖领域的发展。那就是，我们希望成像源具有 对UGA校园的潜水员研究的广泛影响。