Purchase of an imaging flow cytometer 2016

项目概要/摘要： 该提案要求资金购买ImageStream Mark II成像流程 细胞计数共享资源实验室（CSRL）的细胞计数器 格鲁吉亚大学。ImageStream结合了速度、灵敏度和 具有详细成像的流式细胞术的荧光检测能力 和显微镜的功能性见解。这台仪器能产生多个，高- 直接在流动中的每个细胞的分辨率图像，包括明场和暗场 和多达10种荧光标记物。CTEGD Cytometry SRL是中心流 UGA的细胞仪设施，并被安置在UGA中心内，并由UGA中心配备人员 热带和新兴全球疾病（CTEGD）。超过15年 CTEGD CSRL已经为整个大学社区和周边地区提供服务 生物技术公司以及区域学术机构。主要用户 包括以传染病、科学、技术、工程及科学为研究重点的实验室。 细胞分化、环境对细胞功能的影响和肥胖。 在这次购买所支持的传染病研究项目中， 查加斯病是一种影响超过2000万人的疾病， 拉丁美洲和美国，非洲昏睡病和Nagana，一种传染病 每年造成约40亿美元的农业损失，疟疾， 三大传染病杀手病毒免疫学是我们的两个重点 研究宿主基因和病毒之间相互作用的主要用户 因为它与耐药性有关。ImageStream将允许干细胞 和细胞生物学家，以更好地了解细胞周期的进展， 多能干细胞和新的癌症疗法的影响，以及 将细胞周期与环境污染物的影响联系起来。的 通过研究脂肪核获得的知识预计将有助于药物治疗。 肥胖领域的发展。因此，我们期望ImageStream具有 对整个UGA校园的多样化研究的广泛影响。

Project Summary/Abstract: This proposal requests funds to purchase an ImageStream Mark II imaging flow cytometer for the Cytometry Shared Resource Laboratory (CSRL) at the University of Georgia. The ImageStream combines the speed, sensitivity, and fluorescence detection capabilities of flow cytometry with the detailed imaging and functional insights of microscopy. This instrument produces multiple, high- resolution images of every cell directly in flow, including brightfield and darkfield and up to 10 fluorescent markers. The CTEGD Cytometry SRL is the central flow cytometry facility for UGA and is housed within and staffed by the UGA Center for Tropical and Emerging Global Diseases (CTEGD). For more than 15 years the CTEGD CSRL has served the entire university community and surrounding biotechnology companies as well as regional academic institutions. Major users of the facility include laboratories with research foci in infectious diseases, stem cell differentiation, environmental impacts on cellular functions and obesity. Among infectious disease research programs to be supported by this purchase are investigations of Chagas disease, a disease that affects >20 million people in Latin America and the U.S., African sleeping sickness and Nagana, an infection of cattle that results in ~$4 billion in agricultural loss per year, and malaria, one of the “big 3” infectious disease killers. Viral immunology is the focus of 2 of our major users who are studying the interaction between host genes and viral replication as it relates to drug resistance. The ImageStream will allow stem cell and cellular biologists to better understand the cell cycle progression of pluripotent stem cells and the impact of new cancer therapies, as well as to correlate the cell cycle with the effects of environmental pollutants. The knowledge obtained by studying adipose nuclei is projected to facilitate drug development in the field of obesity. Thus, we expect the ImageStream to have a broad impact on the diverse research across the UGA campus.