Inducing Dental Implant Bone Formation to Treat Peri-implantitis

项目摘要摘要 集成有限责任公司的跨学科研究团队通过开发 钛及钛合金种植体的纳米生物活性水基表面涂层 他们在纳米技术、干细胞生物学方面的进展。我们最近公布的数据显示，我们的几个人 多肽与现在用于牙科的钛和钛合金有选择性地结合并具有高亲和力 植入物，并用于矫正轴骨和颅面骨骨缺损和关节置换。使用这个 植入表面结合多肽作为锚与另一个小蛋白质分子相结合使我们能够 引入一种生物活性功能。这里我们选择了一个概念的证明，一个我们发现的小分子 瞬间激活Wnt途径，促进成骨。其他生物活性功能，如抗菌药物 控制生物膜也是设想的。这项技术是以水为基础的双功能小分子 该方法用于控制生物活性多肽或蛋白质在植入物上的呈现 接口，以确保细胞的生物活性。这项前景看好的纳米技术-生物活性涂层技术旨在 改善所有患者的预后，包括那些面临高龄挑战或患有系统性疾病的患者 现在不是植入物的候选者，因为他们的高风险地位。涂层还建立在成功的 钛表面以激活WNT信号。基于团队在生物方面积累的专业知识- 纳米技术、材料科学、干细胞生物学、成骨和颅面生物学，我们开发了 在钛或钛合金可植入材料上模拟生物表面特征的多肽 表面，从而产生仿生的硬-软界面，促进细胞附着，而 同时引导附着细胞在单个步骤中分化为成骨细胞系。我们的 前提是通过在种植体表面设计自组织生物活性界面，我们可以进一步 激活规范的Wnt信号通路引导干细胞向成骨细胞分化 并改善骨整合。此外，涂层可用于重新处理先前放置的植入物 由于种植体周围骨丢失而无法延长其使用寿命。目标1.表征稳定性和 我们的仿生界面在活体中使用众所周知的兔模型保留Wnt信号的时间能力 植入物行业。

Project Summary Abstract The interdisciplinary research team at Integrated LLC has addressed a significant clinical need by developing a nanotech-bioactive water-based surface coating for titanium and titanium alloy based implants based on their advances in nanotechnology, stem cell biology. Our recently published data show that several of our peptides bind selectively and with high affinity to titanium and titanium alloys that are now used for dental implants and for correction of axial and craniofacial skeletal bone defects and joint replacements. Using this implant surface binding peptide as an anchor in combination with another small protein molecule enables us to introduce a bioactive function. Here we chose as a proof of concept, a small molecule we discovered that transiently activates the Wnt pathway for osteogenesis. Other bioactive functions such as an anti-microbial to control biofilms is also envisioned. This technology is water-based and the bifunctional small molecule approach serves to control the presentation of the biologically active peptides or protein at the implant interface to ensure cellular bioactivity. This promising nanotech-bioactive coating technology is designed to improve outcomes for all patients, including those challenged with advanced age or systemic diseases that are not now candidates for implants because of their high risk status. The coating also builds on the success of titanium surfaces to activate Wnt signals. Building upon the team`s accumulated expertise in bio- nanotechnology, materials science, stem cell biology, osteogenesis, and craniofacial biology, we developed peptides that mimic the characteristics of a biological surface on titanium or titanium alloy implantable material surfaces, thereby producing a biomimetic hard-soft interface that promotes cell attachment, while simultaneously directing the differentiation of the attached cells to osteogenic lineages in a single step. Our premise is that through the design of a self-organized bioactive interface on the implant surface we can further activate the canonical Wnt signaling pathway to direct the differentiation of stem cells to osteogenic lineages and improve osteointegration. Additionally, the coating can be used to re-treat previously placed implants that are failing due to bone loss from peri-implantitis to extend their useful life. Aim 1. Characterize the stability and temporal capacity of our biomimetic interface to retain Wnt signaling in vivo using a rabbit model well known to the implant industry.