Inducing Dental Implant Bone Formation to Treat Peri-implantitis

项目概要 摘要 Integrated LLC 的跨学科研究团队通过开发解决了重大的临床需求 一种用于钛和钛合金基植入物的纳米技术生物活性水基表面涂层 他们在纳米技术、干细胞生物学方面的进步。我们最近发布的数据显示，我们的一些 肽选择性地并以高亲和力与现在用于牙科的钛和钛合金结合 植入物以及矫正轴向和颅面骨骼缺陷和关节置换。使用这个 植入物表面结合肽作为锚与另一种小蛋白质分子结合使我们能够 引入生物活性功能。在这里，我们选择了一种小分子作为概念证明，我们发现 短暂激活 Wnt 成骨通路。其他生物活性功能，例如抗菌 还设想了控制生物膜。该技术是水基的双功能小分子 该方法用于控制生物活性肽或蛋白质在植入物上的呈现 确保细胞生物活性的接口。这种有前途的纳米技术生物活性涂层技术旨在 改善所有患者的治疗结果，包括那些患有高龄或全身性疾病的患者 由于其高风险状态，现在不适合植入。该涂层还建立在以下成功的基础上： 钛表面激活 Wnt 信号。以团队在生物领域积累的专业知识为基础 我们开发了纳米技术、材料科学、干细胞生物学、成骨作用和颅面生物学 模仿钛或钛合金植入材料上生物表面特征的肽 表面，从而产生促进细胞附着的仿生硬软界面，同时 同时一步引导附着细胞分化为成骨谱系。我们的 前提是通过在种植体表面设计自组织生物活性界面我们可以进一步 激活经典 Wnt 信号通路，引导干细胞向成骨谱系分化 并改善骨整合。此外，涂层还可用于重新处理先前放置的植入物 由于种植体周围炎造成的骨质流失而无法延长其使用寿命。目标 1. 表征稳定性和 我们的仿生接口使用众所周知的兔子模型在体内保留 Wnt 信号传导的时间能力 植入物行业。

Project Summary Abstract The interdisciplinary research team at Integrated LLC has addressed a significant clinical need by developing a nanotech-bioactive water-based surface coating for titanium and titanium alloy based implants based on their advances in nanotechnology, stem cell biology. Our recently published data show that several of our peptides bind selectively and with high affinity to titanium and titanium alloys that are now used for dental implants and for correction of axial and craniofacial skeletal bone defects and joint replacements. Using this implant surface binding peptide as an anchor in combination with another small protein molecule enables us to introduce a bioactive function. Here we chose as a proof of concept, a small molecule we discovered that transiently activates the Wnt pathway for osteogenesis. Other bioactive functions such as an anti-microbial to control biofilms is also envisioned. This technology is water-based and the bifunctional small molecule approach serves to control the presentation of the biologically active peptides or protein at the implant interface to ensure cellular bioactivity. This promising nanotech-bioactive coating technology is designed to improve outcomes for all patients, including those challenged with advanced age or systemic diseases that are not now candidates for implants because of their high risk status. The coating also builds on the success of titanium surfaces to activate Wnt signals. Building upon the team`s accumulated expertise in bio- nanotechnology, materials science, stem cell biology, osteogenesis, and craniofacial biology, we developed peptides that mimic the characteristics of a biological surface on titanium or titanium alloy implantable material surfaces, thereby producing a biomimetic hard-soft interface that promotes cell attachment, while simultaneously directing the differentiation of the attached cells to osteogenic lineages in a single step. Our premise is that through the design of a self-organized bioactive interface on the implant surface we can further activate the canonical Wnt signaling pathway to direct the differentiation of stem cells to osteogenic lineages and improve osteointegration. Additionally, the coating can be used to re-treat previously placed implants that are failing due to bone loss from peri-implantitis to extend their useful life. Aim 1. Characterize the stability and temporal capacity of our biomimetic interface to retain Wnt signaling in vivo using a rabbit model well known to the implant industry.