Polymer Sensors for Rapid Cell Genotyping

点击翻译按钮获取中文摘要

In this proposal, Vuronyx Technologies in collaboration with Prof. Vince Rotello at UMass Amherst aims to develop a prototype kit for validation of cell lines. Cell lines have a wide range of applications in both academic and life sciences industries, including genomics, drug discovery, tissue engineering, regenerative medicine, and toxicity testing. However, cell cultures are prone to contamination by foreign cells, which may rapidly displace the original cells. The identity of cultured cells should be routinely verified, but a majority of laboratories do not monitor the identity of their cell lines, and many cell lines are misidentified. The costs, effort and time required to confirm the identity of cell lines has been a barrier to adoption of cell line identification as a routine quality control measure Our preliminary results show that our pattern-based approach is capable of distinguishing both cell genotype and phenotype. Our research will focus on developing a ‘minimalist’ polymer- only sensor array that can rapidly discriminate between cell types and geno/phenotypes. The polymer platform presents a scalable and reproducible platform, an advantage that will be furthered through close collaboration between Rotello and Vuronyx. The objective in Phase 1 is to develop the polymer nanoparticle based cell sensing strategy and test it against a range of cell types. This proposal is directly relevant to NIH’s mission of fostering creative discoveries for human well-being, as well as NIGMS’s mission of understanding of biological processes and advancing disease diagnosis, treatment and prevention.