New mechanism of commensal bacteria interaction with host immunity

共生菌与宿主免疫相互作用的新机制

• 批准号: 9317868
• 负责人: Ivaylo Ivanov Ivanov
• 金额: $ 24万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2019-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9317868
• 关键词: Adherence; Adhesions; Antigen-Presenting Cells; Antigens; Bacteria; Bacterial Antigens; Bacterial Proteins; Biological Process; CD4 Positive T Lymphocytes; Carrier Proteins; Cell membrane; Cells; Clathrin; Collection; Communication; Cytoplasm; Endocytic Vesicle; Endocytosis; Epithelial; Epithelial Cells; Eukaryotic Cell; Feces; Gastrointestinal tract structure; Generations; Gram-Negative Bacteria; Human; Immune response; Immunity; In Vitro; Individual; Inflammation; Injection of therapeutic agent; Intestines; Mediating; Mediator of activation protein; Membrane; Microbe; Molecular; Morphology; Mouse Strains; Mucosal Immunity; Mucous Membrane; Mucous body substance; Nature; Organism; Pathway interactions; Peptides; Phagocytosis; Physiological; Play; Process; Production; Proteins; Reactive Oxygen Species; Role; Route; Secretory Immunoglobulin A; Surface; Symbiosis; Synapses; System; T-Lymphocyte; Tissues; Toxin; Vesicle; Virus; antimicrobial; chemical genetics; commensal microbes; cytokine; experimental study; genetic approach; glycosylation; immunoregulation; in vivo; innate immune function; interest; intestinal homeostasis; microbial; microbiota; novel; pathogen; protein transport; receptor; response; tool

How commensal bacteria communicate with the host to modify immunity is incompletely understood. Here we propose to characterize a previously unrecognized pathway of communication between bacteria and the eukaryotic host and its role in mediating immunomodulatory effects of mucosa-associated commensals. To identify the nature of the process we will characterize the involvement and requirement of various endocytic pathways by a combination of chemical and genetic approaches. We will also generate and examine these interactions in a novel in vitro system. If successful, our studies will uncover a novel mechanism for delivery of bacterial antigens for activation of commensal-specific T cells in the intestine, and/or for delivery of bacterial molecules for immune modulation.

肠道细菌如何与宿主沟通以改变免疫力尚不完全清楚。 在这里，我们提出了一个以前未被认识的细菌之间的沟通途径的特点 和真核宿主及其在介导粘膜相关免疫调节作用中的作用 - 谢谢为了确定过程的性质，我们将描述参与和要求 各种内吞途径的化学和遗传方法的组合。我们还将 在一个新的体外系统中产生和检查这些相互作用。 如果成功的话，我们的研究将揭示一种新的细菌抗原传递机制， 激活肠中的结肠特异性T细胞，和/或用于递送细菌分子， 免疫调节