Development Core

开发核心

• 批准号: 9814482
• 负责人: TIMOTHY RAYMOND FENNELL
• 金额: $ 38.15万
• 依托单位: RESEARCH TRIANGLE INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-05 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9814482
• 关键词: Big Data Methods; Biological; Biological Markers; Chemical Exposure; Chemicals; Coupled; Detection; Development; Ensure; Environmental Exposure; Feces; Food; Gas Chromatography; Health; Human; Ingestion; Institution; Laboratories; Libraries; Liquid substance; Mass Fragmentography; Mass Spectrum Analysis; Measures; Metabolism; Methods; Natural Products; Pesticides; Pharmaceutical Preparations; Phenols; Plants; Plasma; Polychlorinated Biphenyls; Procedures; Protocols documentation; Reference Standards; Resolution; Resources; Saliva; Sampling; Schedule; Serum; Services; Signal Transduction; Source; Sweat; Techniques; Testing; Tissues; Toxic effect; Toxicology; Translating; U-Series Cooperative Agreements; Urine; Work; base; biomarker development; data acquisition; drug of abuse; epidemiology study; experience; in vivo; liquid chromatography mass spectrometry; mass spectrometer; multiple reaction monitoring; novel strategies; polybrominated diphenyl ether; polyphenol; sample collection; working group

Project Summary/Abstract (Development Core) The Development Core (DC) and the Untargeted Analysis Resource Core (UARC) will collaborate, with the overall aims to increase the range of analytes or biological markers that are detected and identified that reflect environmental exposures. The DC will work with the Big Data Analytics of UARC to prioritize the sourcing of compounds. The DC will optimize extraction procedures for new matrices, in addition to the matrices already analyzed (e.g., urine, serum, plasma, feces, saliva and sweat), and will conduct analyses of biospecimens collected and stored under different conditions to inform best practices for epidemiology studies. They will establish an untargeted method using gas chromatography coupled to an orbitrap mass spectrometer (GC-Q- Exactive) to expand the services offered by the UARC. The DC will also use GC- and liquid chromatography (LC)-Mass spectrometry with Multiple Reaction Monitoring (MRMs) and MSn to develop specific broad spectrum panels for rapid quantitative analysis of analytes that are difficult to detect on the untargeted platforms. The DC will be directed by Dr. Timothy Fennell (MPI, RTI), who has extensive experience with chemical exposure, metabolite identification and toxicity, and exposure biomarker development.

项目摘要/摘要(开发核心) 开发核心(DC)和非目标分析资源核心(UARC)将与 总体目标是增加检测和鉴定的分析物或生物标志物的范围，以反映 暴露在环境中。数据中心将与UARC的大数据分析合作，确定采购的优先顺序 化合物。除了已有的矩阵外，DC还将优化新矩阵的提取程序 分析(例如尿液、血清、血浆、粪便、唾液和汗液)，并将进行生物检疫分析 在不同条件下收集和储存，为流行病学研究提供最佳做法。他们会 建立气相色谱与Orbitrap质谱仪(GC-Q-MS)联用的非靶向方法 Exactive)，以扩大UARC提供的服务。DC还将使用GC-和液相色谱 (LC)-多反应监测(MRMS)和MSN的质谱学开发特定的广谱 光谱板，用于快速定量分析在非靶标上难以检测到的分析物 站台。DC将由Timothy Fennell博士(MPI，RTI)指导，他拥有丰富的 化学暴露、代谢物鉴定和毒性，以及暴露生物标志物的开发。