Expression and Impact of Interspersed Repeats
散布重复序列的表达和影响
基本信息
- 批准号:9816738
- 负责人:
- 金额:$ 32.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-07-24 至 2020-02-29
- 项目状态:已结题
- 来源:
- 关键词:AffectAllelesAmberAreaBindingCellsCellular biologyComplexConflict (Psychology)DNADNA Insertion ElementsDNA MethylationDNA Transposable ElementsDataDevelopmentDiseaseDisease modelDouble-Stranded RNAElementsEmbryoEndogenous RetrovirusesEpigenetic ProcessExperimental ModelsFertilityGene ExpressionGene Expression RegulationGene SilencingGenesGeneticGenetic DiseasesGenetic TranscriptionGenomeHeterochromatinHumanImmune responseIndividualInfectious AgentInterferon Type IILong Interspersed ElementsMeasuresMessenger RNAModernizationMolecularMutagensMutationNuclear ExportOutcomePathway interactionsPhenotypeProliferatingProteinsQuantitative Trait LociRNARNA EditingRegulationRetroelementsRetrotranspositionRetrotransposonReverse TranscriptionRoleSETDB1 geneShort Interspersed Nucleotide ElementsSideSignal TransductionSiteSurveysTestingTissuesTrans-ActivatorsTranscriptTranscriptional RegulationVariantZinc Fingersdifferential expressiongenetic variantgenome editinggenome wide association studyhuman diseasehuman tissuemRNA Exportpathogenrecruitresponsesensortranscriptome sequencing
项目摘要
Summary
Endogenous retroviruses and related transposable elements make up much of our genome, and active forms
persist as long interspersed element-1 (LINE-1, L1) retrotransposons and the elements it mobilizes. These are
self-propagating sequences which copy themselves through the reverse transcription of RNA intermediates.
We have developed elaborate mechanisms to silence their transcription and limit their proliferation. However, a
careful approach to RNAseq analysis reveals that that a subset of individual transposon loci (ITL) escape
silencing and are transcriptionally active. In a survey of normal human cells, we identify both constitutive and
variably expressed ITL. Our ability to measure these transcripts provides a unique opportunity to examine this
host-pathogen relationship and its role in gene regulation. Here, we propose to test the hypothesis that
retroelement expression (or lack thereof) is an indicator of specific regulatory mechanisms with cis-acting
effects on neighboring genes. We propose to define the landscape of expressed retrotransposons in humans;
demonstrate molecular mechanisms of transcriptional silencing and escape; and probe how these mechanisms
affect gene expression in human ‘epigenetic’ diseases. We will use genome editing strategies to test whether
altering ITL sequences affects neighboring gene expression. Finally, we will develop approaches to study
prevalent, highly-expressed short interspersed elements (SINEs) to assess their potential for
retrotransposition, determine whether they are targets of RNA editing or dsRNA sensing pathways, and test
their interactions with cellular mRNAs in trans. These studies will be among the first to delve into the
expression and function of a broad range of retrotransposon intermediates in human cells.
总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
KATHLEEN H BURNS其他文献
KATHLEEN H BURNS的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('KATHLEEN H BURNS', 18)}}的其他基金
Consequences of retrotransposition on genome integrity
逆转录转座对基因组完整性的影响
- 批准号:
10736406 - 财政年份:2023
- 资助金额:
$ 32.75万 - 项目类别:
Characterizing the LINE-1 Retrotransposition-Replication Conflict
表征 LINE-1 逆转录转座-复制冲突
- 批准号:
10215445 - 财政年份:2020
- 资助金额:
$ 32.75万 - 项目类别:
Characterizing the LINE-1 Retrotransposition-Replication Conflict
表征 LINE-1 逆转录转座-复制冲突
- 批准号:
10634604 - 财政年份:2020
- 资助金额:
$ 32.75万 - 项目类别:
Characterizing the LINE-1 Retrotransposition-Replication Conflict
表征 LINE-1 逆转录转座-复制冲突
- 批准号:
10440427 - 财政年份:2020
- 资助金额:
$ 32.75万 - 项目类别:
Opportunities for Pathology Trainees in Cancer Research
癌症研究病理学实习生的机会
- 批准号:
9006475 - 财政年份:2015
- 资助金额:
$ 32.75万 - 项目类别:
Mouse Models of Functional Insertion Polymorphisms
功能插入多态性的小鼠模型
- 批准号:
8423827 - 财政年份:2013
- 资助金额:
$ 32.75万 - 项目类别:
相似海外基金
Linkage of HIV amino acid variants to protective host alleles at CHD1L and HLA class I loci in an African population
非洲人群中 HIV 氨基酸变异与 CHD1L 和 HLA I 类基因座的保护性宿主等位基因的关联
- 批准号:
502556 - 财政年份:2024
- 资助金额:
$ 32.75万 - 项目类别:
Olfactory Epithelium Responses to Human APOE Alleles
嗅觉上皮对人类 APOE 等位基因的反应
- 批准号:
10659303 - 财政年份:2023
- 资助金额:
$ 32.75万 - 项目类别:
Deeply analyzing MHC class I-restricted peptide presentation mechanistics across alleles, pathways, and disease coupled with TCR discovery/characterization
深入分析跨等位基因、通路和疾病的 MHC I 类限制性肽呈递机制以及 TCR 发现/表征
- 批准号:
10674405 - 财政年份:2023
- 资助金额:
$ 32.75万 - 项目类别:
An off-the-shelf tumor cell vaccine with HLA-matching alleles for the personalized treatment of advanced solid tumors
具有 HLA 匹配等位基因的现成肿瘤细胞疫苗,用于晚期实体瘤的个性化治疗
- 批准号:
10758772 - 财政年份:2023
- 资助金额:
$ 32.75万 - 项目类别:
Identifying genetic variants that modify the effect size of ApoE alleles on late-onset Alzheimer's disease risk
识别改变 ApoE 等位基因对迟发性阿尔茨海默病风险影响大小的遗传变异
- 批准号:
10676499 - 财政年份:2023
- 资助金额:
$ 32.75万 - 项目类别:
New statistical approaches to mapping the functional impact of HLA alleles in multimodal complex disease datasets
绘制多模式复杂疾病数据集中 HLA 等位基因功能影响的新统计方法
- 批准号:
2748611 - 财政年份:2022
- 资助金额:
$ 32.75万 - 项目类别:
Studentship
Genome and epigenome editing of induced pluripotent stem cells for investigating osteoarthritis risk alleles
诱导多能干细胞的基因组和表观基因组编辑用于研究骨关节炎风险等位基因
- 批准号:
10532032 - 财政年份:2022
- 资助金额:
$ 32.75万 - 项目类别:
Recessive lethal alleles linked to seed abortion and their effect on fruit development in blueberries
与种子败育相关的隐性致死等位基因及其对蓝莓果实发育的影响
- 批准号:
22K05630 - 财政年份:2022
- 资助金额:
$ 32.75万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigating the Effect of APOE Alleles on Neuro-Immunity of Human Brain Borders in Normal Aging and Alzheimer's Disease Using Single-Cell Multi-Omics and In Vitro Organoids
使用单细胞多组学和体外类器官研究 APOE 等位基因对正常衰老和阿尔茨海默病中人脑边界神经免疫的影响
- 批准号:
10525070 - 财政年份:2022
- 资助金额:
$ 32.75万 - 项目类别:
Leveraging the Evolutionary History to Improve Identification of Trait-Associated Alleles and Risk Stratification Models in Native Hawaiians
利用进化历史来改进夏威夷原住民性状相关等位基因的识别和风险分层模型
- 批准号:
10689017 - 财政年份:2022
- 资助金额:
$ 32.75万 - 项目类别: