The generation, migration and function of inflammatory ILC2s

炎症ILC2的产生、迁移和功能

• 批准号: 9817116
• 负责人: Yuefeng Huang
• 金额: $ 24.9万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-05 至 2021-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9817116
• 关键词: Adoptive Transfer; Allergic inflammation; Amphiregulin; Antigens; Area; Ascaris suum; Autoimmune Diseases; Autoimmunity; Award; Biogenesis; Biology; Blood Circulation; Bone Marrow; Candidate Disease Gene; Career Mobility; Cells; Cellular biology; Chronic; Communication; Complement; Data; Development; Environment; Fatty acid glycerol esters; Fetal Liver; Flow Cytometry; Gene Expression Profile; Generations; Genes; Goals; Grant; Histology; Homeostasis; Immune; Immune response; Immunity; Immunologist; Immunology; In Vitro; Infection; Infectious Agent; Inflammation; Inflammatory; Innate Immune System; International; Intestines; Investigation; Knockout Mice; Laboratories; Lamina Propria; Learning; Leukocytes; Lung; Lymphocyte; Lymphoid; Lymphoid Cell; Mediating; Mentors; Metabolic; Modeling; Mus; National Institute of Allergy and Infectious Disease; Nature; Nippostrongylus; Organogenesis; Paper; Parabiosis; Phase; Play; Population; Positioning Attribute; Postdoctoral Fellow; Process; Proliferating; Publishing; Reporter; Reporting; Research; Research Personnel; Role; Scientist; Secure; Site; Small Intestines; Stem cells; Structure of parenchyma of lung; System; Systems Biology; Technology; Testing; Therapeutic; Training; Transgenic Mice; United States National Institutes of Health; Update; Work; Writing; ZNF145 gene; adaptive immunity; cell motility; chemokine receptor; cytokine; fungus; improved; influenzavirus; inhibitor/antagonist; intraperitoneal; knockout gene; migration; mouse model; pathogen; progenitor; receptor; repaired; skills; tenure track; tissue repair; trafficking; transcriptome sequencing

Project Summary/Abstract Innate lymphoid cells (ILCs) are recently identified constituents of innate immune system and have been the focus of intense investigation over the past five years. ILCs provide early immune protection against infectious agents, mediate lymphoid organogenesis and tissue repair, participate in the transition from innate to adaptive immunity, contribute to inflammation and autoimmunity, repair tissue damage and regulate metabolic homeostasis. My long-term goal is to establish a productive research group and ultimately to become a leading scientist in the area of ILCs, especially type 2 ILCs (ILC2s). My prior study has reported the existence of an inflammatory ILC2 (iILC2) population that is transient ILC progenitors mobilized by inflammation and infection; they are capable of developing into natural ILC2-like cells or ILC3-like cells and contributing to immunity to both helminthes and fungi (Huang et al., Nature Immunology 2015). The principle aim of this proposal is to elucidate the generation, migration and function of iILC2s. I will use multiple experimental technologies to identify the progenitors, understand the process of iILC2 trafficking and investigate iILC2 function in tissue repair and metabolic homeostasis. The initial phase of this project will be conducted in Laboratory of Immunology and Laboratory of System Biology NIAID, which provide a superb environment to fulfill my trainings and research. My mentor, Dr. Ronald Germain, is a world-leading immunologist. He is an NIH Distinguished Investigator and has published more than 300 scholarly research papers and reviews. He serves as an associate or advisory editor of the J Exp Med, Immunity, Current Biology, Mol Systems Biol, BMC Biology, Nature Communications, eLife, and Int Immunol, and has previously served as Deputy Editor of J Immunol and Editor, Immunity. He has received numerous awards and honors. He has trained dozens of postdoctoral fellows, many of whom now occupy senior academic posts and are internationally recognized investigators. I will take the advantage of NIH and Dr. Germain's laboratory to enhance my intellectual background of immunology, to expand my scope of scientific research, to learn necessary experimental technologies, and to improve my skills on grant writing mentoring, lab management and scientific communication. All these activities will secure my career transition from a postdoc fellow to an independent tenure-track faulty position.

项目摘要/摘要 先天淋巴样细胞(ILCs)是新近发现的先天免疫系统的组成部分，已成为 过去五年密集调查的重点。ILC提供早期免疫保护，防止感染 调节淋巴器官发生和组织修复的物质参与了从先天到适应性的转变 免疫，促进炎症和自身免疫，修复组织损伤和调节代谢 动态平衡。我的长期目标是建立一个富有成效的研究小组，并最终成为一个领先的 ILC领域的科学家，特别是第二类ILC(ILC2)。我之前的研究报告了一种 炎症性ILC2(IILC2)群体是由炎症和感染动员的一过性ILC前体细胞； 它们能够发育成天然的ILC2样细胞或ILC3样细胞，并有助于免疫 蠕虫和真菌(Huang等人，《自然免疫学》2015)。这项建议的主要目的是 阐明iILC2的产生、迁移和功能。我将使用多种实验技术来 鉴定前体细胞，了解iILC2的转运过程，并研究iILC2在组织中的功能 修复和代谢动态平衡。该项目的第一阶段将在 免疫学和系统生物学实验室，为我的工作提供了一个极好的环境 培训和研究。我的导师罗纳德·杰曼博士是一位世界领先的免疫学家。他是美国国立卫生研究院的 杰出的研究员，发表了300多篇学术研究论文和评论。他发球 作为J Exp Med，免疫，当代生物学，分子系统生物学，BMC的副主编或顾问编辑 生物学、自然通讯、eLife和Int免疫学，并曾担任过J 《免疫》杂志和编辑，《免疫》。他获得了许多奖项和荣誉。他已经培训了数十名 博士后研究员，其中许多人现在担任高级学术职位并得到国际认可 调查人员。我将利用NIH和Germain博士的实验室来提高我的智力 免疫学背景，为了扩大我的科研范围，学习必要的实验 技术，并提高我在拨款撰写指导、实验室管理和科学研究方面的技能 沟通。所有这些活动将确保我的职业生涯从博士后过渡到独立人士 终身教职跟踪错误的职位。