Whole brain circuit mapping of transplanted interneurons following traumatic brain injury

点击翻译按钮获取中文摘要

PROJECT SUMMARY/ABSTRACT The loss of inhibitory interneurons after traumatic brain injury has been associated with the development of epilepsy and other deficits in cognition. Our lab has previously demonstrated that embryonic interneuron progenitors transplanted into the brain migrate, differentiate, and functionally integrate into host-brain networks, and can suppress spontaneous seizures and ameliorate learning and memory deficits in a mouse model of epilepsy. Transplanted interneurons exhibit mature morphologies and electrophysiological properties characteristic of endogenous interneurons. However, the cell types and brain regions that innervate transplanted interneurons are currently unknown. The goal of this proposal is to use a rabies virus tracing strategy, whole-brain clearing, and intact-brain imaging to map cell-type specific monosynaptic inputs onto interneurons grafted into the healthy and injured hippocampus.