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Whole brain circuit mapping of transplanted interneurons following traumatic brain injury

脑外伤后移植中间神经元的全脑回路图谱

基本信息

批准号：
10012950
负责人：
Jan Christopher Frankowski
金额：
$ 4.13万
依托单位：
UNIVERSITY OF CALIFORNIA-IRVINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-30 至 2021-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10012950
关键词：
Adult Affect Apoptotic Attention Behavior Behavioral Brain Brain Injuries Brain imaging Brain region Cell Death Cell Therapy Cell Transplantation Cells Characteristics Cholecystokinin Closed head injuries Cognition Cognitive Cognitive deficits Development Electrophysiology (science)Embryo Epilepsy Evaluation Exhibits Genes Goals Hippocampus (Brain)Image Injury Interneurons Learning Light Maps Memory Memory impairment Microscopy Modeling Modernization Morphology Mus Necrosis Neurodegenerative Disorders Parvalbumins Pattern Phase Population Post-Traumatic Epilepsy Property Rabies Rabies virus Reporter Reporting Research Personnel Seizures Severities Somatostatin Synapses Techniques Testing Therapeutic Time Tissues Transplantation Traumatic Brain Injury Vasoactive Intestinal Peptide Viral Virus Visualization axon injury brain cell career cell type controlled cortical impact density hippocampal subregions injured mouse model nervous system disorder neurochemistry novel therapeutics progenitor skills

项目摘要

PROJECT SUMMARY/ABSTRACT The loss of inhibitory interneurons after traumatic brain injury has been associated with the development of epilepsy and other deficits in cognition. Our lab has previously demonstrated that embryonic interneuron progenitors transplanted into the brain migrate, differentiate, and functionally integrate into host-brain networks, and can suppress spontaneous seizures and ameliorate learning and memory deficits in a mouse model of epilepsy. Transplanted interneurons exhibit mature morphologies and electrophysiological properties characteristic of endogenous interneurons. However, the cell types and brain regions that innervate transplanted interneurons are currently unknown. The goal of this proposal is to use a rabies virus tracing strategy, whole-brain clearing, and intact-brain imaging to map cell-type specific monosynaptic inputs onto interneurons grafted into the healthy and injured hippocampus.

项目摘要/摘要创伤性脑损伤后抑制性中间神经元的丢失与脑损伤的发生有关。癫痫和其他认知缺陷。我们的实验室之前已经证明了胚胎中间神经元移植到大脑中的祖细胞迁移、分化并在功能上整合到宿主-大脑网络中，并能抑制自发性癫痫发作，改善小鼠学习记忆障碍。癫痫。移植的中间神经元表现出成熟的形态和电生理特性具有内源性中间神经元的特点。然而，神经的细胞类型和大脑区域移植的中间神经元目前还不清楚。这项提案的目标是使用狂犬病病毒追踪策略、全脑清除和完整脑成像将细胞类型的特定单突触输入映射到中间神经元被移植到健康和受损的海马区。