Pathogenesis of hERG Mutations in Human Long QT Syndrome

人类长 QT 综合征 hERG 突变的发病机制

• 批准号: 9243297
• 负责人: ZHENGFENG ZHOU
• 金额: $ 38.5万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-05 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9243297
• 关键词: Accounting; Action Potentials; Antineoplastic Agents; Arrhythmia; C-terminal; Cardiac; Cardiac Myocytes; Cells; Clinical; Dependovirus; Development; Disease; Electrocardiogram; Functional disorder; Funding; Genes; Genotype; Goals; Heart; Human; Introns; Knock-in Mouse; Knowledge; Lead; Long QT Syndrome; Molecular; Mutation; Pathogenesis; Patients; Pharmaceutical Preparations; Phenotype; Play; Polyadenylation; Potassium Channel; Pre-mRNA Polyadenylation Factor; Protein Isoforms; RNA Interference; RNA Splicing; Regulation; Reporter; Risk; Role; Serotyping; Small Nuclear RNA; Sudden Death; Testing; Tissues; Trans-Activators; Transfection; Up-Regulation; Ventricular Arrhythmia; Vorinostat; in vivo; induced pluripotent stem cell; mouse model; novel; novel therapeutics; public health relevance

 DESCRIPTION (provided by applicant): Long QT syndrome is a disorder characterized by delayed cardiac repolarization and an increased risk of arrhythmias and sudden death. Both congenital and acquired (such as drug-induced) forms of long QT syndrome have been identified. Long QT syndrome type 2 (LQT2) is caused by mutations in the KCNH2 gene (also known as hERG1 or hERG). KCNH2 encodes the Kv11.1 K+ channel that conducts the rapidly activating delayed rectifier K+ current (IKr) in the heart. The Kv11.1 channel plays an important role in congenital and drug-induced forms of long QT syndrome. LQT2 is the second most prevalent form of congenital long QT syndrome accounting for 35 to 40% of genotyped cases of long QT syndrome. Many drugs cause drug- induced long QT syndrome by inhibition of Kv11.1 channel function. Kv11.1 channel dysfunction results in the delay in action potential repolarization, leading to QT prolongation and cardiac arrhythmias. In the previous funding period, we have identified isoform switch as a novel mechanism of Kv11.1 channel dysfunction in LQT2. We also showed that the relative expression of Kv11.1 C-terminal isoforms plays an important role in the regulation of Kv11.1 channel function, and developed an antisense approach to increase the functional Kv11.1 isoform expression. In the present application, we will study the mechanisms underling the developmental and tissue-specific regulation of Kv11.1 isoform expression and demonstrate the modulation of Kv11.1 isoform expression as a novel mechanism of drug-induced suppression of Kv11.1 channel function. In addition, we will test the antisense-induced upregulation of Kv11.1 channel function in induced pluripotent stem cell-derived cardiomyocytes and a knock-in mouse model. The specific aims of this application are: Aim 1) To study the mechanisms underlying the tissue-specific and developmental regulation of Kv11.1 isoform expression. Aim 2) To identify the modulation of the relative expression Kv11.1 isoforms as a novel mechanism of drug-induced suppression of Kv11.1 channel function. Aim 3) To study the antisense-induced upregulation of functional Kv11.1 isoform expression in LQT2 patient-specific induced pluripotent stem cell-derived cardiomyocytes that carry different KCNH2 mutations. Aim 4) To study the antisense-induced upregulation of functional Kv11.1 isoform expression in a knock-in mouse model. The knowledge gained from this study will strengthen our understanding of the molecular mechanisms of long QT syndrome and provide information directed towards the development of novel therapeutic strategies for long QT syndrome.

 描述（由申请方提供）：长QT综合征是一种以心脏复极延迟和心律失常和猝死风险增加为特征的疾病。先天性和获得性（如药物诱导）长QT综合征的形式都已确定。长QT综合征2型（LQT 2）是由KCNH 2基因（也称为hERG 1或hERG）突变引起的。KCNH 2编码Kv11.1 K+通道，该通道传导心脏中快速激活的延迟整流K+电流（IKr）。Kv11.1通道在先天性和药物诱导的长QT综合征中起着重要作用。LQT 2是先天性长QT综合征的第二种最常见形式，占长QT综合征基因分型病例的35%至40%。许多药物通过抑制Kv11.1通道功能引起药物性长QT综合征. Kv11.1通道功能障碍导致动作电位复极延迟，导致QT间期延长和心律失常。在上一个资助期，我们已经确定亚型转换是LQT 2中Kv11.1通道功能障碍的一种新机制。我们还发现，Kv11.1 C-末端亚型的相对表达在Kv11.1通道功能的调节中起着重要作用，并开发了一种反义方法来增加功能性Kv11.1亚型的表达。在本申请中，我们将研究Kv11.1亚型表达的发育和组织特异性调节的机制，并证明Kv11.1亚型表达的调节是药物诱导的Kv11.1通道功能抑制的新机制。此外，我们将在诱导多能干细胞衍生的心肌细胞和敲入小鼠模型中测试反义诱导的Kv11.1通道功能上调。本申请的具体目的是：目的1）研究Kv11.1亚型表达的组织特异性和发育调节的潜在机制。目的2）探讨药物抑制Kv11.1通道功能的新机制：Kv11.1亚型相对表达的调节。目的3）研究反义Kv11.1在携带不同KCNH 2突变的LQT 2患者特异性诱导多能干细胞来源的心肌细胞中的表达上调。目的4）在基因敲入小鼠模型中研究反义Kv11.1亚型表达的上调。从这项研究中获得的知识将加强我们对长QT综合征的分子机制的理解，并为长QT综合征的新治疗策略的发展提供信息。