ShEEP Request for Fluorescence-Activated Cell Sorter (FACS)

ShEEP 请求荧光激活细胞分选仪 (FACS)

• 批准号: 9361791
• 负责人: THOMAS J HORNYAK
• 金额: --
• 依托单位: BALTIMORE VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-01-01 至 2017-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9361791
• 关键词: Adverse effects; Aerosols; Agreement; Air; Area; Arthritis; Autoimmune Diseases; Autoimmune Process; Award; B-Lymphocytes; Baltimore; Brain-Derived Neurotrophic Factor; Calibration; Cancer Immunology Science; Cell Separation; Cells; Characteristics; Clinic; Collaborations; Color; Computer software; Controlled Study; Core Facility; Custom; Demyelinating Diseases; Devices; Disease; Disease model; Doctor of Medicine; Doctor of Philosophy; Equipment; Experimental Autoimmune Encephalomyelitis; Flow Cytometry; Fluorescein; Fluorescence; Funding; Gold; Health; Healthcare Systems; Hematology; Human; IRAK1 gene; Immune; Immunosuppressive Agents; Individual; Inflammation; Inflammatory; Interleukins; Isothiocyanates; K-Series Research Career Programs; Laboratories; Lasers; Learning; Lymphocyte; Maintenance; Malignant Neoplasms; Maryland; Mediating; Medical center; Minor; Mus; Nerve Regeneration; Optics; Pamphlets; Patients; Phycoerythrin; Plant Roots; Population; Price; Principal Investigator; Property; Pulmonary Fibrosis; Reagent; Regenerative Medicine; Reproducibility of Results; Research; Research Personnel; Sampling; Serum; Services; Sheep; Skin; Sorting - Cell Movement; Spleen; Stem cells; System; Technical Expertise; Temperature; Testing; Tissues; Training; Tube; Veterans; Viola; Work; base; cell type; data management; data sharing; experience; experimental study; falls; fluorescence activated cell sorter device; fluorophore; instrument; interest; macrophage; melanocyte; neoplastic cell; pathogen; price lists; programs; repaired; research and development; rheumatologist; therapy resistant; tumor; tumor immunology; user-friendly

The project is a ShEEP request for a Becton Dickinson (BD) FACSMelodyTM Cell Sorter. The BD FACSMelodyTM Cell Sorter is a high quality flow cytometry sorting instrument, with an automated instrument setup routine, that has the advantage of being easy to learn and use. The unit provides researchers a supportive and comprehensive workflow experience, and is derived from the cited and proven BD FACS Aria Cell Sorter. Typically the system is ready in less than 17 minutes- maximizing uptime. The small unit creates streamlined workflow with instrument settings, as well as gates and sort settings available and retrievable from saved experiments. The cell sorter system anticipates potential issues and identifies root causes with filter/mirror units that communicate with the software to confirm the optical configuration supports for experiments. The instrument will facilitate individual VA investigators with widely varying projects to isolate individual types of cells from normal and disease tissue and study their characteristics, leading to advances in the understanding of tissue inflammation, cancer immunology, and nervous system regeneration. The characteristics of the instrument make it ideal for our VA Research Service because this award will support the work of 5 major VA Merit-funded users (Drs. Hornyak, Atamas, Luzina, Davila, and Makar), and a minor user (Dr. Golding) who is a Career Development Award (CDA-2) recipient. Thomas J. Hornyak, M.D., Ph.D., is the Principal Investigator (PI) for this proposal. He is the Associate Chief Of Staff (ACOS) for Research and Development (R&D) at the VA Maryland Health Care System (VAMHCS), and PI on the BLR&D Merit Review project “Melanocyte Stem Cells in Regenerative Medicine”. He and his laboratory will use the sorter to isolate subsets of both mouse and human skin-derived stem cells in the viable state to evaluate their properties to promote neural regeneration. Sergei Atamas, M.D., Ph.D., and Irina Luzina, M.D., Ph.D., are individually funded VA Merit Review investigators with laboratories located in the Baltimore VA Medical Center (VAMC) who collaborate on the study of interleukin (IL)-33 and pulmonary fibrosis. They will sort tissue- derived macrophages and lymphocytes with the FACSMelodyTM. Eduardo Davila, Ph.D., is a VA Merit Review investigator whose research program is focused on tumor immunology. He will use the cell sorter for his VA Merit Review project to isolate tumor cells expressing varying levels of IRAK-4, postulated to mediate tumor resistance to therapy. Tapas Makar, Ph.D., is a VA Merit-funded investigator who is interested in determining the ability of brain-derived neurotrophic factor (BDNF) to modulate murine experimental autoimmune encephalitis, a disease model for autoimmune demyelinating disease in humans. He will sort immune and inflammatory cells from mouse spleen after experimental treatments to evaluate the immunosuppressive side effects of those treatments. Amit Golding, M.D., Ph.D. is a VAMHCS rheumatologist and a CDA awardee that treats Veterans in the Baltimore VAMC Arthritis Clinic. He submitted a VA BLR&D Merit Review application (Fall 2016 Cycle) subsequent to his CDA-2 project; and has a research program emphasizing autoimmune disease. Dr. Golding will sort B lymphocytes and T regulatory cells from serum samples from patients with autoimmune disease and normal controls for his studies.

该项目是对Becton Dickinson(BD)FACSMelodyTM细胞分选机的绵羊请求。屋宇署 FACSMelodyTM细胞分选仪是一种高质量的流式细胞仪分选仪，具有自动 仪器设置例程，具有易学易用的优点。该单位提供 研究人员拥有支持性和全面的工作流经验，并源自引用 和久经考验的BD FACS Aria细胞分选器。通常情况下，系统在不到17分钟内准备就绪- 最大限度延长正常运行时间。小型单位还通过仪器设置创建了简化的工作流程 因为门和分类设置可从保存的实验中获得和检索。该细胞分选器 系统可预测潜在问题，并通过符合以下条件的过滤器/镜像单元确定根本原因 与软件进行通信，确认实验所需的光学配置支持。这个 仪器将帮助退伍军人管理局的个人调查人员进行差异很大的项目，以隔离个人 正常组织和疾病组织的细胞类型并研究它们的特征，导致在 了解组织炎症、癌症免疫学和神经系统再生。 该仪器的特点使其成为我们退伍军人管理局研究服务的理想选择，因为该奖项 将支持退伍军人福利基金资助的5个主要用户(Hornyak博士、Atamas博士、Luzina博士、Davila博士和 Makar)，以及职业发展奖(CDA-2)获得者的次要用户(戈尔丁博士)。 Thomas J.Hornyak，医学博士，是该提案的首席调查员(PI)。他就是那个 退伍军人事务部马里兰卫生局负责研发(R&D)的副参谋长(ACO) 关爱系统(VAMHCS)和PI在BLR&D功绩审查项目“黑素细胞干细胞在 再生医学“。他和他的实验室将使用分选器分离这两只小鼠的亚群 并对人皮肤来源的干细胞在存活状态下的性能进行评估，以促进神经 再生。谢尔盖·阿塔马斯医学博士和伊琳娜·卢齐娜医学博士是个人资助的退伍军人管理局 位于巴尔的摩退伍军人医疗中心(VAMC)的实验室的功绩审查调查人员 世卫组织合作研究白介素33与肺纤维化。他们会对组织进行分类- 来源的巨噬细胞和淋巴细胞与FACSMelodyTM。爱德华多·达维拉博士是退伍军人管理局 功绩审查调查员，他的研究项目集中在肿瘤免疫学上。他将使用 他的VA Merit Review项目的细胞分选器，分离表达不同水平IRAK-4的肿瘤细胞， 被认为可以调节肿瘤对治疗的抵抗。Tapas Makar博士是退伍军人管理局奖励计划资助的 对测定脑源性神经营养因子(BDNF)能力感兴趣的研究人员 对自身免疫性疾病模型小鼠实验性自身免疫性脑炎的调控 人类的脱髓鞘疾病。他将从小鼠的脾中分离免疫细胞和炎症细胞 在实验治疗后评估这些治疗的免疫抑制副作用。 阿米特·戈尔丁，医学博士，VAMHCS风湿病专家，CDA奖获得者 巴尔的摩VAMC关节炎诊所。他提交了退伍军人事务部BLR&D功绩审查申请(秋季 在他的CDA-2项目之后)；并有一个研究计划，强调 自身免疫性疾病。戈尔丁博士将从血清中对B淋巴细胞和T调节细胞进行分类 来自自身免疫性疾病患者和正常对照的样本，用于他的研究。