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Charge sensitive optical detection for high-throughput study of small molecules

用于小分子高通量研究的电荷敏感光学检测

基本信息

批准号：
9316572
负责人：
NONGJIAN TAO
金额：
$ 38.97万
依托单位：
ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2019-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9316572
关键词：
Address Affinity Antineoplastic Agents Automation Binding Biochemical Reaction Biological Markers Biomedical Research Biosensing Techniques Cell physiology Charge Collaborations Detection Diagnosis Drug Targeting Drug resistance Electrons Fiber Fluorescence Fluorescent Dyes Geometry Grant Kinetics Label Malignant Neoplasms Manufacturer Name Measures Mechanics Membrane Proteins Methods Molecular Analysis Molecular Weight Mutate Noise Optics Output Peptide Library Performance Pharmaceutical Preparations Pharmacologic Substance Phosphotransferases Post-Translational Protein Processing Preclinical Drug Evaluation Procedures Process Proteins Reaction Reading Research Screening for cancer Serum Signal Transduction Surface System Techniques Technology Testing Time Translations United States National Institutes of Health Validation Work anticancer research biomarker discovery cancer biomarkers cancer initiation cancer therapy commercialization computerized data processing cost data acquisition design drug candidate drug development electric field electronic data high throughput screening innovation instrument laboratory development molecular mass new technology novel strategies optical fiber receptor research and development screening small molecule success tool tumor progression

项目摘要

PROJECT SUMMARY A technology that can detect and quantify the kinetics of small molecule binding and biochemical reactions, such as post-translational protein modifications, is critical for understanding the mechanisms underlying cancer initiation and progression, for discovering cancer biomarkers, and for screening cancer drug candidates. Currently, the most widely used technology uses fluorescence labels, which is difficult for small molecules with sizes comparable to the fluorescent dyes, especially for quantitative kinetic information. Various label-free techniques have been developed, but their sensitivity diminishes with the molecular mass, making it extremely challenging to detect small molecules and biochemical reactions that involve small mass changes. To address this need, a charge sensitive optical detection (CSOD) technology will be developed in this project. CSOD is compatible with the standard microplate technology, making it attractive for high-throughput screening and analysis of molecular interactions and reactions. The basic principle was established with the support of an IMAT (Innovative Molecular Analysis Technologies for cancer research) R21 grant. In the present R33 project, the team will work closely with collaborators in academic research labs and pharmaceutical companies, and a bioanalytical instrument company to develop and validate the technology for the unmet need. The success of the project will lead to a new label-free high-throughput screening technology for measuring molecular interactions, particularly small molecule interactions and post-translational modifications. These processes are highly important for the research, diagnosis and treatment of cancer.

项目总结