Engineering Core

工程核心

基本信息

批准号：
9444235
负责人：
ARAVINTHAN D. SAMUEL
金额：
$ 24.52万
依托单位：
HARVARD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9444235
关键词：
Atlases Behavior Behavioral Biological Assay Biological Models Brain Calcium Collection Complex Computer software Custom Data Data Analyses Data Engineering Decision Making Development Devices Drosophila genus Electrophysiology (science)Engineering Equipment Goals Histology Image Invertebrates Laboratories Microscope Modeling Movement Neurons Neurosciences Neurosciences Research Optics Play Postdoctoral Fellow Rattus Research Rodent Role Savings Science Scientist Slice Stretching Structure Students Swimming System Tissues Training Training and Education United States National Institutes of Health Vision Zebrafish base cost effective cost effectiveness data acquisition design design and construction experimental study instrumentation laboratory experience light microscopy meetings member millimeter neural circuit novel prototype relating to nervous system student training two-photon

项目摘要

Engineering Core-Abstract The goal of the Engineering Core is to enable our Team members to surmount their most challenging technological and engineering obstacles. There are many needs for engineering solutions in our proposal--from behavioral apparatus to optical and electrophysiological rigs, to software for data acquisition and analyses. Even the most technologically sophisticated of the research groups in our Team require special assistance with engineering problems in order to achieve our ambitious aims. In the Center for Brain Science at Harvard there is an existing Engineering Core that consists of (a) an integrated laboratory to design, prototype, iterate, and produce custom devices for neuroscience experiments and (b) an engineer-neuroscientist who solves the most challenging engineering problems in the user laboratories and trains young scientists to do this themselves. By adding a dedicated staff engineer, the Engineering Core will be able to support the team-based neuroscience research proposed here. The specific aims are to implement engineering solutions to problems faced by the Projects and other Cores. The Engineering Core will (a) design and build apparatuses to image rapidly swimming larval zebrafish; (b) build other apparatuses to quantitatively assay complex zebrafish behavior; (c) build rigs to record neural activity in larval zebrafish brains via calcium imaging; (d) fabricate rigs to record neural activity in larval Drosophila and rat; and modify software for data acquisition in many existing experimental apparatus, including microscopes.

工程核心提取工程核心的目标是使我们的团队成员能够克服他们最挑战的技术和工程障碍。我们的提案中有许多工程解决方案的需求 - 从行为仪器到光学和电生理钻机，用于用于数据获取和分析的软件。甚至技术上最多的我们团队中研究小组的精致需要在工程问题方面进行特殊帮助才能实现我们雄心勃勃的目标。在哈佛大学的脑科学中心，有一个现有的工程核心包括（a）集成实验室，用于设计，原型，迭代和生产用于神经科学实验的定制设备，并且（b）工程师 - 神经科学家解决了用户实验室中最挑战的工程问题并训练年轻的工程问题科学家自己这样做。通过添加专门的员工工程师，工程核心将能够支持基于团队的神经科学研究在这里提出。具体目的是将工程解决方案实施到问题面对项目和其他核心。工程核心将（a）设计和构建设备以快速形象游泳幼虫斑马鱼；（b）构建其他仪器以定量测定复杂的斑马鱼行为；（c）建造钻机通过钙成像记录幼虫斑马鱼大脑中的神经元活性；（d）制造钻机以记录幼虫的神经元活动果蝇和大鼠；并修改许多现有实验设备中的数据获取软件，包括显微镜。