Circuit mechanisms underlying cortical communications

皮层通信的电路机制

• 批准号: 9568287
• 负责人: Soohyun Lee
• 金额: $ 123.23万
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9568287
• 关键词: Animal Behavior; Animals; Area; Behavior; Behavioral; Biological Models; Brain; Cerebral cortex; Cognition; Communication; Communication impairment; Complex; Cues; Data; Development; Electrophysiology (science); Environment; Esthesia; Genetic; Genetic Techniques; Goals; Image; Lead; Mediator of activation protein; Memory; Mental disorders; Methods; Minority; Molecular Genetics; Monitor; Movement; Neurons; Perception; Pharmacogenetics; Population; Process; Recruitment Activity; Research; Research Infrastructure; Rodent; Role; Sensory; Somatosensory Cortex; Specificity; Stream; Synapses; System; Vibrissae; Viral; cell type; design; experience; experimental study; gamma-Aminobutyric Acid; imaging modality; interdisciplinary approach; neuroregulation; optogenetics; sensorimotor system; sensory cortex; tool

In starting a new unit, we have been building infrastructure, designing and conducting our experiments, and collecting preliminary data. First, to achieve mechanistic understanding of the functional connectivity among different cortical areas, we have started systematically examining the synaptic strength from different brain areas to diverse neuronal types in the primary somatosensory cortex, using electrophysiological methods combined with genetic tools. Second, to identify distinct microcircuits recruited by these different long-range inputs, we have been setting up imaging methods to monitor and manipulate activity of specific neuronal populations. Third, to understand the behavioral relevance of these circuits, we have been designing different types of behavior tasks that allow us to understand the role of these circuits in animals behaviors. The current hypothesis in our lab is that long-range projections from higher-order cortical and neuromodulatory areas recruit specific subtypes of GABAergic neurons in sensory cortex. As a key mediator, this distinct set of GABAergic neurons engage a specific local microcircuit.

在开始一个新的单位，我们一直在建设基础设施，设计和进行我们的实验，并收集初步数据。首先，为了从机制上了解不同皮质区域之间的功能连接，我们开始使用电生理方法与遗传工具相结合，系统地检查从不同大脑区域到初级躯体感觉皮质中不同神经元类型的突触强度。其次，为了识别这些不同的长程输入所招募的不同微电路，我们一直在建立成像方法来监测和操纵特定神经元群体的活动。第三，为了了解这些回路的行为相关性，我们一直在设计不同类型的行为任务，使我们能够了解这些回路在动物行为中的作用。 我们实验室目前的假设是，来自高阶皮层和神经调节区的长程投射招募感觉皮层中特定亚型的GABA能神经元。作为一个关键的调解人，这套独特的GABA能神经元参与一个特定的局部微电路。