Circuit mechanisms underlying cortical communications

皮层通信的电路机制

• 批准号: 10266632
• 负责人: Soohyun Lee
• 金额: $ 196.75万
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10266632
• 关键词: Animal Behavior; Animals; Area; Axon; Behavior; Behavioral; Biological Models; Brain; Calcium; Cells; Characteristics; Cognition; Communication; Communication impairment; Electrophysiology (science); Elements; Feedback; Genetic Techniques; Goals; Heterogeneity; Image; Interneurons; Manuscripts; Mediating; Memory; Mental disorders; Methods; Molecular Genetics; Movement; Neocortex; Neurons; Perception; Pharmacogenetics; Rabies virus; Research; Rodent; Role; Sensory; Somatosensory Cortex; Specificity; Synapses; System; Vibrissae; Viral; base; information processing; inhibitory neuron; interdisciplinary approach; neural circuit; optogenetics; organizational structure; presynaptic; programs; recruit; sensorimotor system; sensory cortex

In order to understand the principles of long-range connectivity in cortical communication, our efforts have focused on the following two projects during FY20. Functional connectivity of diverse long-range inputs to sensory cortex is aimed at achieving a mechanistic understanding of the functional connectivity of feedback / top-down projections to the primary sensory cortex. We have systematically examined the synaptic strength from different brain areas to diverse neuronal types in the primary somatosensory cortex (S1) and determined how the primary sensory cortex uses input-area-dependent, preferential recruitment of specific types of GABAergic interneurons to parse information from diverse feedback projections. The results of this study are currently under review. The second project, The structural organization of cortical subnetworks, is aimed at understanding the principles that govern the functional heterogeneity of principal neurons in sensory cortex. Neuronal activity in the superficial layer of the primary sensory cortex is highly heterogenous in relation to various aspects of the animals behavior. We asked whether functionally heterogeneous subnetworks are constrained by specific long-range and local presynaptic ensembles. This study provides the circuit-based mechanism for the organization of cortical subnetworks. Using single cell-initiated, monosynaptic rabies virus tracing combined with calcium imaging, we found that behavior state-encoding (spontaneous movement) neurons show characteristic long-range and local presynaptic networks. Our results reveal connectivity rules that support functional heterogeneity of cortical principal cells. We are currently preparing a manuscript on this study.

为了了解皮层通信中的远程连接原理，我们在2020财年重点开展了以下两个项目。 各种远程输入到感觉皮层的功能连接旨在实现对反馈/自上而下投射到初级感觉皮层的功能连接的机械理解。我们系统地研究了初级躯体感觉皮层（S1）中不同脑区与不同神经元类型的突触强度，并确定了初级感觉皮层如何使用输入区依赖性，优先招募特定类型的GABA能中间神经元来解析来自不同反馈投射的信息。目前正在审查这项研究的结果。 第二个项目，皮层子网络的结构组织，旨在了解控制感觉皮层中主要神经元功能异质性的原则。初级感觉皮层浅层的神经元活动与动物行为的各个方面有关，具有高度的异质性。我们问是否功能异质的子网络的限制特定的长程和局部突触前合奏。本研究为皮层子网络的组织提供了基于回路的机制。使用单细胞启动的，单突触狂犬病病毒示踪结合钙成像，我们发现，行为状态编码（自发运动）神经元显示出特征性的长程和局部突触前网络。我们的研究结果揭示了支持皮层主细胞功能异质性的连接规则。我们目前正在准备一份关于这项研究的手稿。