Drosophila Transcription Factor Interactome

果蝇转录因子相互作用组

• 批准号: 9042394
• 负责人: MARTHA L BULYK
• 金额: $ 38.8万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-10 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9042394
• 关键词: Address; Affect; Animal Model; Animals; Binding; Binding Proteins; Binding Sites; Biological Assay; Biological Models; Biological Process; Cell Cycle; Cells; Cis Tests; Code; Collaborations; Communities; Complex; Computer Analysis; DNA Binding; DNA-Protein Interaction; Data; Data Set; Development; Disease; Drosophila genus; Drosophila melanogaster; Family; Functional disorder; Gene Expression; Gene Expression Profile; Gene Expression Regulation; Gene Targeting; Genetic Complementation Test; Health; Human; Individual; Lead; Letters; Literature; Maps; Nature; Organism; Output; Property; Proteins; Regulator Genes; Regulatory Element; Resources; Role; Source; Specificity; Technology; Tissues; Transcriptional Regulation; Yeasts; cofactor; combinatorial; cost effectiveness; genome-wide; in vivo; preference; protein protein interaction; response; screening; transcription factor; yeast two hybrid system

DESCRIPTION (provided by applicant): The interactions between transcription factors (TFs) and their DNA binding sites are an integral part of the gene regulatory networks within cells. Although many TFs have had their DNA binding specificities determined in recent years in various organisms, and while combinatorial regulation of gene expression by TFs is well known in animal development, we still have a poor understanding of how protein-protein interactions (PPIs) affect TF DNA binding specificities and effects on the expression of their target genes. In this project, we will focus on Drosophila melanogaster as a convenient, experimentally powerful model organism with significant transcriptional regulatory complexity. Specifically, we will investigate: (1) what TFs physically interact with each other, such as to form heterodimers? (2) do TFs have different DNA binding preferences individually versus as complexes? (3) do different TF interaction partners result in different binding properties of the TFs in vivo or associate with different biological processes? Altogether, these studies will lead to a better understanding of how PPIs among sequence-specific TFs affect TF regulatory specificity. These studies also will provide a valuable resource of datasets for the community.

描述（由申请人提供）： 转录因子 (TF) 与其 DNA 结合位点之间的相互作用是细胞内基因调控网络的组成部分。尽管近年来许多转录因子在各种生物体中的 DNA 结合特异性已被确定，并且转录因子对基因表达的组合调控在动物发育中已众所周知，但我们对蛋白质-蛋白质相互作用 (PPI) 如何影响 TF DNA 结合特异性以及对其靶基因表达的影响仍然知之甚少。在这个项目中，我们将重点关注果蝇作为一种方便的、实验上强大的模型生物，具有显着的转录调控复杂性。具体来说，我们将研究：（1）哪些转录因子在物理上相互作用，例如形成异二聚体？ (2) TF 单独与作为复合物时是否具有不同的 DNA 结合偏好？ (3) 不同的TF相互作用伙伴是否会导致TF在体内的不同结合特性或与不同的生物过程相关？总而言之，这些研究将有助于更好地理解序列特异性 TF 中的 PPI 如何影响 TF 调控特异性。这些研究还将为社区提供宝贵的数据集资源。