Drosophila Transcription Factor Interactome

描述（由申请人提供）： 转录因子与其DNA结合位点之间的相互作用是细胞内基因调控网络的组成部分。尽管近年来在各种生物体中已经确定了许多TF的DNA结合特异性，而TF对基因表达的组合调控在动物发育中是众所周知的，但我们仍然对蛋白质-蛋白质相互作用（PPI）如何影响TF DNA结合特异性及其对靶基因表达的影响了解甚少。在这个项目中，我们将专注于果蝇作为一个方便的，实验功能强大的模式生物具有显着的转录调控复杂性。具体来说，我们将研究：（1）什么样的TF相互作用，如形成异二聚体？(2)TF单独与作为复合物具有不同的DNA结合偏好吗？(3)不同的TF相互作用伴侣是否导致TF在体内的不同结合特性或与不同的生物学过程相关？总之，这些研究将导致更好地了解PPI之间的序列特异性TF影响TF调节特异性。这些研究也将为社区提供宝贵的数据集资源。

DESCRIPTION (provided by applicant): The interactions between transcription factors (TFs) and their DNA binding sites are an integral part of the gene regulatory networks within cells. Although many TFs have had their DNA binding specificities determined in recent years in various organisms, and while combinatorial regulation of gene expression by TFs is well known in animal development, we still have a poor understanding of how protein-protein interactions (PPIs) affect TF DNA binding specificities and effects on the expression of their target genes. In this project, we will focus on Drosophila melanogaster as a convenient, experimentally powerful model organism with significant transcriptional regulatory complexity. Specifically, we will investigate: (1) what TFs physically interact with each other, such as to form heterodimers? (2) do TFs have different DNA binding preferences individually versus as complexes? (3) do different TF interaction partners result in different binding properties of the TFs in vivo or associate with different biological processes? Altogether, these studies will lead to a better understanding of how PPIs among sequence-specific TFs affect TF regulatory specificity. These studies also will provide a valuable resource of datasets for the community.