Scientific Core 2 (SC2) Cell and Protein Preparation Core

摘要 SC2 Scientific Core 2表达PPG三个项目所必需的蛋白质。将蛋白质 主要在细菌和昆虫细胞中表达。然后对蛋白质进行纯化和表征， 它们被正确折叠并具有功能活性。SC2还构建了用于表达蛋白质的载体， 哺乳动物细胞验证所有构建体的序列。这些高质量的试剂随后被转交给 在拟议的实验中使用的项目。超过50种蛋白质和50种DNA载体将在 核心的通过集中这些活动，可以共享通用试剂和专业知识并保持一致 可以确保共享试剂的行为和结果。科学核心2还为PPG提供培训 在细菌和哺乳动物细胞中的蛋白质表达，蛋白质纯化和表征方面的人员 和诱变策略。

Abstract SC2 Scientific Core 2 expresses proteins that are essential for the three Projects of the PPG. The proteins are expressed primarily in bacteria and also insect cells. The proteins are then purified and characterized to test that they are properly folded and functionally active. SC2 also constructs vectors for expression of proteins in mammalian cells. Sequences of all constructs are verified. These high-quality reagents are then turned over to the projects for utilization in proposed experiments. Over 50 proteins and 50 DNA vectors will be constructed in the Core. By centralizing these activities, common reagents and expertise can be shared and consistent behavior of shared reagents and outcomes can be assured. Scientific Core 2 also provides training to PPG personnel in to protein expression in bacteria and mammalian cells, protein purification and characterization and in mutagenesis strategies.