Tools and Methods for the Elucidation of Fatty Acid Amide Cell Signaling Pathways

阐明脂肪酸酰胺细胞信号通路的工具和方法

• 批准号: 10310497
• 负责人: James Leahy
• 金额: $ 17.66万
• 依托单位: UNIVERSITY OF SOUTH FLORIDA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-05 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10310497
• 关键词: Active Sites; Afferent Neurons; Alkynes; Amides; Amines; Amino Acids; Anabolism; Antibodies; Area; Binding; Binding Proteins; Biogenic Amines; Biological Models; Biomedical Research; CNR1 gene; Calcium; Carrier Proteins; Cells; Coupled; Data; Development; Drosophila melanogaster; Drug Targeting; Ensure; Enzymes; Family; Fatty Acids; Funding; Future; Goals; Health; Human; Individual; Invertebrates; Investigation; Label; Ligand Binding; Ligands; Lipids; Locomotion; Mechanics; Membrane; Metabolism; Methods; Modification; Mus; N-palmitoylglycine; Neuroblastoma; Nitric Oxide; Nucleotides; Orphan; Parents; Pharmaceutical Preparations; Play; Positioning Attribute; Process; Production; Proteins; Proteome; Proteomics; Research; Role; Signal Pathway; Signal Transduction; Sleep; Specificity; Structure; System; Tissues; United States National Institutes of Health; Validation; Vertebrates; Visualization; Work; analog; anandamide; base; bropirimine; design; drug discovery; experience; fatty acid-transport protein; flexibility; improved; in vivo; innovative technologies; insight; long chain fatty acid; member; new therapeutic target; novel; oleylamide; receptor; receptor binding; tool

Abstract: Cell signaling is one of the most fundamental processes that controls human health, and yet the mechanics of this intricate system is largely unappreciated. This is stunning because the primary agents for this system, membrane receptors, are known to account for approximately 1/3 of all approved human drugs. Often, the receptors themselves are known before the discovery of their endogenous ligands. In this proposal, we detail a systematic method for the identification of the targets of fatty acid amides, a highly regulated class of molecules known to engage in at least two signaling processes. The bulk of the other members of this class of compounds have never been paired with their receptors, and uncovering this information has tremendous potential for identifying new therapeutic targets and improving drug discovery efforts by revealing potentially undesirable off-target candidates.

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