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Expanding the known coding genome: identifying biological function for novel tORFs

扩展已知的编码基因组：识别新型 tORF 的生物学功能

基本信息

批准号：
10308710
负责人：
Sidney Wang
金额：
$ 30.33万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-12-01 至 2025-11-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10308710
关键词：
5&apos Untranslated Regions Active Sites Amino Acids Area Biological Biological Process Biology Biomedical Research CRISPR library CRISPR screen Catalogs Categories Cell Culture Techniques Cell Survival Classification Clustered Regularly Interspaced Short Palindromic Repeats Code Codon Nucleotides Computing Methodologies Culture Media DNA Sequence Data Dropout Drosophila genus Evaluation Event Evolution Foundations Frequencies Future Genes Genetic Genetic Diseases Genetic Predisposition to Disease Genetic Screening Genetic study Genome Genomic Segment Genomics Goals Guide RNA Human Human Genome Initiator Codon Lead Link Literature Macaca mulatta Mass Spectrum Analysis Medicine Mendelian disorder Messenger RNA Methods Modernization Molecular Molecular Weight Mus Natural Selections Open Reading Frames Pan Genus Pathway interactions Pattern Peptides Periodicity Play Preparation Primates Production Proteins Proteomics Protocols documentation Public Health Research Research Design Ribosomes Role Sampling Sequence Alignment Shotguns Signal Transduction Skeletal Muscle Supervision Testing Transcript Translating Translations Untranslated RNA Untranslated Regions Vertebrates base design experimental study fitness follow-up functional genomics genetic variant genome annotation genome wide association study genome-wide genomic data improved loss of function lymphoblastoid cell line muscle physiology novel programs reverse genetics ribosome profiling tandem mass spectrometry transcriptome

项目摘要

Abstract Annotations of coding genes in the human genome have been tremendously useful in understanding etiology of genetic disorders and in basic biology research. Despite being the most accurate and comprehensive set of genomic features annotated, emerging evidence has indicated that an increasing number of translated regions are missing from the current annotation. These overlooked genomic regions, or formally translated open reading frames (tORFs), represents important biology missing from the current literature. For example, myoregulin, a conserved 46 amino acid micro-peptide was discovered in a “non-coding” region, and was later demonstrated to function in regulating skeletal muscles in mice. These potentially functional novel tORFs are often small, and therefore overlooked by most coding gene annotation programs. To overcome this challenge, efforts leveraging functional genomics datasets to identify novel coding regions across the human genome have begun to reveal this previously underappreciated class of genomic features. In particular, the applicants previously developed a computational method, riboHMM, which leverages patterns specific to the translated regions in functional genomics data, such as ribo-seq data, in order to identify tORFs genome-wide. Using riboHMM to systematically annotate tORFs in human lymphoblastoid cell lines, 7,273 novel tORFs were found, in addition to the tORFs of known coding genes. These novel tORFs were found in regions of the transcriptome previously annotated as non-coding (e.g. Untranslated Regions and lincRNAs). Although newly developed methods, such as riboHMM, can now systematically identify thousands of previously overlooked tORFs, the biological relevance of these translation events remains unclear. The objective of the current proposal is to evaluate functional relevance for these newly discovered tORFs. Three major aspects of biological importance will be evaluated. First, loss of function impact. Effects of tORF deletion on cell viability and synthetic fitness impact in combination with well- characterized coding genes will be evaluated using pooled CRISPR dropout screens (Aim 1). Second, ability to encode protein/peptide. The ability of tORFs to produce stable protein/peptide will be evaluated in mass spectrometry studies designed for detecting translation products of small ORFs (Aim 2). Third, evolution conservation. The strength of purifying selection on these loci will be carefully evaluated using new alignments created based on independently annotated novel tORFs in chimpanzee and rhesus macaque. The completion of the proposed aims will provide the first systematic evaluation of biological relevance for novel tORFs. Impacts of these new functional annotations could range from providing new interpretations for GWAS hits to reevaluating “non-coding RNA” function. Results from the proposed study will guide future research directions on this group of previously overlooked genomic features. Given the sheer number of unexplored tORFs and the prior examples of overlooked tORFs that turned out to play critical roles in important biological pathways, the findings here will have far reaching implications for both basic and translational biomedical research.

摘要