Experimental and Analytical Resource Core

实验和分析资源核心

• 批准号: 10310691
• 负责人: Liz Simon
• 金额: $ 97.35万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10310691
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Address; Adherence; Adult; Adverse effects; Affect; Age; Aging; Alcohol consumption; Alcohols; Behavioral; Biological; Biological Assay; Caring; Center for Translational Science Activities; Chronic; Clinical; Clinical Data; Clinical Research; Clinical Sciences; Communities; Complement; Consumption; Data; Databases; Dietary Practices; Elderly; Enrollment; Ensure; Environmental Risk Factor; Ethics; Experimental Designs; Extramural Activities; Funding; Guidelines; HIV; HIV Seronegativity; HIV therapy; Health; Health Sciences; Healthcare; Human; Human Resources; Human Subject Research; Individual; Investigation; Longitudinal Studies; Louisiana; Macaca mulatta; Measures; Mediating; Mediator of activation protein; Methodology; Mission; Modeling; NCI Center for Cancer Research; National Institute on Alcohol Abuse and Alcoholism; Older Population; Outcome; Pathway interactions; Performance; Persons; Pilot Projects; Postdoctoral Fellow; Pre-Clinical Model; Procedures; Process; Protocols documentation; Quality Control; Regimen; Reproducibility; Research; Research Personnel; Research Project Grants; Research Support; Resources; Risk; SIV; Sampling; Services; Social Environment; Specimen; Study Subject; Study models; Teacher Professional Development; Testing; Time; Training; Translational Research; United States National Institutes of Health; Universities; Update; Vertebrates; age related; alcohol comorbidity; alcohol exposure; alcohol risk; alcohol use disorder; analytical tool; animal care; antiretroviral therapy; biobank; cohort; comorbidity; design; experience; human subject; instrumentation; male; next generation; nonhuman primate; novel; pre-clinical research; preclinical study; recruit; research study; social factors; translational scientist; translational study

Abstract LSUHSC CARC Experimental and Analytical Core The Experimental and Analytical Core provides essential services in support of the research conducted by the Louisiana State University Health Sciences Center (LSUHSC) Comprehensive Alcohol-HIV/AIDS Research Center (CARC). The Core will facilitate bidirectional translational cross-sectional and longitudinal investigations of at-risk alcohol use and comorbidities among in-care persons living with HIV (PLWH) enrolled in the New Orleans Alcohol Use in HIV (NOAH) Study and in a new cohort of seronegative individuals. Mechanistic studies in chronic binge alcohol (CBA) simian immunodeficiency virus (SIV)-infected male rhesus macaques will complement clinical studies. The Core will provide an integrated platform for biospecimen storage and analysis of diverse biospecimens collected from clinical and preclinical studies. Major strengths of the Core include established, quality controlled clinical and nonhuman primate (NHP) experimental protocols, analytical tools, sophisticated instrumentation, and the potential to develop and implement novel assays to test hypotheses. The responsibilities of the Core will be guided by the four specific aims: (1) To operationalize and conduct bidirectional translational research studies on alcohol-mediated mechanisms impacting health outcomes in PLWH; (2) To collect, process, and biobank biospecimens from the translational studies; (3) To analyze biospecimens and expedite transfer of clinical data and assay results to the CARC database (operated within the Administrative Core); and (4) To train faculty, pre- and postdoctoral fellows, and staff on alcohol-related translational research and the analysis of biological samples. Thus, the Core will support studies proposed by CARC investigators through (a) ensuring regulatory compliance for all NHP and human subject studies; (b) facilitating bidirectional translational opportunities for basic and clinical researchers; (c) ensuring quality control in processing and storage of biospecimens and guaranteeing data collected from longitudinal studies are consistent over time; (d) centralizing performance of all assays to ensure quality control and reproducibility of analytical procedures; (e) ensuring that limiting samples are used for the highest priority multiple assays; and f) training personnel and trainees in the use of NHPs, basics of clinical research, and state-of-art analytical procedures. The Core will synergize with existing LSUHSC resources including the Louisiana Clinical and Translational Science (LA CaTS) Center, Division of Animal Care, and the Louisiana Cancer Research Center to meet these objectives. The Core will leverage on the expertise, experience, and resources to guarantee best practices and quality control and adherence to regulatory guidelines. Centralizing CARC experimental and analytical activities ensures that rigor, integrity, and transparency in the design and conduct of human and preclinical research is maintained across all CARC projects.

摘要LSUHSC CARC实验与分析核心 实验和分析核心提供必要的服务，以支持 路易斯安那州立大学健康科学中心(LSUHSC)综合酒精-艾滋病毒/艾滋病研究 中心(CARC)。核心将促进双向平移、横断面和纵向调查 在新登记的护理艾滋病毒携带者(PLWH)中的高危酒精使用和共病 奥尔良酒精在HIV(诺亚)研究和新的血清阴性个体队列中的使用。机械学研究 在慢性酗酒(CBA)猴免疫缺陷病毒(SIV)感染的雄性猕猴会 补充临床研究。该核心将为生物量储存和分析提供一个综合平台 从临床和临床前研究中收集的各种生物标本。核心的主要优势包括 建立有质量控制的临床和非人类灵长类(NHP)实验方案、分析工具、 精密的仪器，以及开发和实施新的分析方法来检验假说的潜力。这个 核心的责任将以四个具体目标为指导：(1)实施和开展双向 关于酒精对PLWH健康结局影响机制的转化研究；(2) 从翻译研究中收集、加工和生物库生物样品；(3)分析生物样品和 加快将临床数据和化验结果传输到CARC数据库(在行政部门内运行 核心)；和(4)培训教员、博士后和博士后研究员以及与酒精相关的翻译研究工作人员 以及生物样本的分析。因此，核心将支持CARC调查人员提出的研究 通过(A)确保所有国家健康方案和人体研究遵守法规；(B)促进双向 为基础和临床研究人员提供翻译机会；(C)确保加工和生产过程中的质量控制 储存生物量并保证从纵向研究中收集的数据随时间保持一致；(D) 集中所有化验的性能，以确保质量控制和分析程序的重现性；(E) 确保将限量样品用于最高优先级的多次化验；以及f)培训人员和 学员学习如何使用NHP、临床研究的基础知识和最新的分析方法。核心意志 与现有LSUHSC资源协同，包括路易斯安那州临床和转化科学(LA CATS) 动物保育部和路易斯安那州癌症研究中心，以实现这些目标。《核心》 将利用专业知识、经验和资源来确保最佳实践和质量控制 遵守监管准则。集中CARC实验和分析活动可确保严谨、 在人类和临床前研究的设计和进行中保持完整性和透明度 CARC项目。