Experimental and Analytical Resource Core

实验和分析资源核心

• 批准号: 10310691
• 负责人: Liz Simon
• 金额: $ 97.35万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10310691
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Address; Adherence; Adult; Adverse effects; Affect; Age; Aging; Alcohol consumption; Alcohols; Behavioral; Biological; Biological Assay; Caring; Center for Translational Science Activities; Chronic; Clinical; Clinical Data; Clinical Research; Clinical Sciences; Communities; Complement; Consumption; Data; Databases; Dietary Practices; Elderly; Enrollment; Ensure; Environmental Risk Factor; Ethics; Experimental Designs; Extramural Activities; Funding; Guidelines; HIV; HIV Seronegativity; HIV therapy; Health; Health Sciences; Healthcare; Human; Human Resources; Human Subject Research; Individual; Investigation; Longitudinal Studies; Louisiana; Macaca mulatta; Measures; Mediating; Mediator of activation protein; Methodology; Mission; Modeling; NCI Center for Cancer Research; National Institute on Alcohol Abuse and Alcoholism; Older Population; Outcome; Pathway interactions; Performance; Persons; Pilot Projects; Postdoctoral Fellow; Pre-Clinical Model; Procedures; Process; Protocols documentation; Quality Control; Regimen; Reproducibility; Research; Research Personnel; Research Project Grants; Research Support; Resources; Risk; SIV; Sampling; Services; Social Environment; Specimen; Study Subject; Study models; Teacher Professional Development; Testing; Time; Training; Translational Research; United States National Institutes of Health; Universities; Update; Vertebrates; age related; alcohol comorbidity; alcohol exposure; alcohol risk; alcohol use disorder; analytical tool; animal care; antiretroviral therapy; biobank; cohort; comorbidity; design; experience; human subject; instrumentation; male; next generation; nonhuman primate; novel; pre-clinical research; preclinical study; recruit; research study; social factors; translational scientist; translational study

Abstract LSUHSC CARC Experimental and Analytical Core The Experimental and Analytical Core provides essential services in support of the research conducted by the Louisiana State University Health Sciences Center (LSUHSC) Comprehensive Alcohol-HIV/AIDS Research Center (CARC). The Core will facilitate bidirectional translational cross-sectional and longitudinal investigations of at-risk alcohol use and comorbidities among in-care persons living with HIV (PLWH) enrolled in the New Orleans Alcohol Use in HIV (NOAH) Study and in a new cohort of seronegative individuals. Mechanistic studies in chronic binge alcohol (CBA) simian immunodeficiency virus (SIV)-infected male rhesus macaques will complement clinical studies. The Core will provide an integrated platform for biospecimen storage and analysis of diverse biospecimens collected from clinical and preclinical studies. Major strengths of the Core include established, quality controlled clinical and nonhuman primate (NHP) experimental protocols, analytical tools, sophisticated instrumentation, and the potential to develop and implement novel assays to test hypotheses. The responsibilities of the Core will be guided by the four specific aims: (1) To operationalize and conduct bidirectional translational research studies on alcohol-mediated mechanisms impacting health outcomes in PLWH; (2) To collect, process, and biobank biospecimens from the translational studies; (3) To analyze biospecimens and expedite transfer of clinical data and assay results to the CARC database (operated within the Administrative Core); and (4) To train faculty, pre- and postdoctoral fellows, and staff on alcohol-related translational research and the analysis of biological samples. Thus, the Core will support studies proposed by CARC investigators through (a) ensuring regulatory compliance for all NHP and human subject studies; (b) facilitating bidirectional translational opportunities for basic and clinical researchers; (c) ensuring quality control in processing and storage of biospecimens and guaranteeing data collected from longitudinal studies are consistent over time; (d) centralizing performance of all assays to ensure quality control and reproducibility of analytical procedures; (e) ensuring that limiting samples are used for the highest priority multiple assays; and f) training personnel and trainees in the use of NHPs, basics of clinical research, and state-of-art analytical procedures. The Core will synergize with existing LSUHSC resources including the Louisiana Clinical and Translational Science (LA CaTS) Center, Division of Animal Care, and the Louisiana Cancer Research Center to meet these objectives. The Core will leverage on the expertise, experience, and resources to guarantee best practices and quality control and adherence to regulatory guidelines. Centralizing CARC experimental and analytical activities ensures that rigor, integrity, and transparency in the design and conduct of human and preclinical research is maintained across all CARC projects.

摘要 LSUHSC CARC 实验和分析核心 实验和分析核心提供必要的服务来支持研究所进行的研究 路易斯安那州立大学健康科学中心 (LSUHSC) 酒精-艾滋病毒/艾滋病综合研究 中心（CARC）。该核心将促进双向平移横断面和纵向研究 参加新计划的艾滋病毒感染者 (PLWH) 护理人员中高危饮酒者和合并症的比例 奥尔良艾滋病毒（NOAH）研究和新的血清阴性人群中的酒精使用情况。机理研究 在慢性酗酒（CBA）、猿猴免疫缺陷病毒（SIV）感染的雄性恒河猴中， 补充临床研究。该核心将为生物样本存储和分析提供一个集成平台 从临床和临床前研究中收集的各种生物样本。核心的主要优势包括 已建立的、质量受控的临床和非人类灵长类动物 (NHP) 实验方案、分析工具、 先进的仪器，以及开发和实施新颖的分析方法来检验假设的潜力。这 核心的职责将遵循四个具体目标： (1) 实施和开展双向 关于酒精介导的影响艾滋病病毒感染者健康结果的机制的转化研究； (2) 至 从转化研究中收集、处理和生物样本库生物样本； (3) 分析生物样本 加快将临床数据和化验结果传输至 CARC 数据库（在行政管理部门内运行） 核）; (4) 对教师、博士前和博士后研究员以及酒精相关转化研究工作人员进行培训 以及生物样本的分析。因此，核心将支持 CARC 研究人员提出的研究 通过 (a) 确保所有 NHP 和人类受试者研究符合法规； (b) 促进双向 为基础和临床研究人员提供转化机会； (c) 确保加工过程中的质量控制 生物样本的存储并保证从纵向研究中收集的数据随着时间的推移保持一致； (四) 集中所有测定的性能，以确保分析程序的质量控制和再现性； （五） 确保限制样本用于最高优先级的多重检测； f) 培训人员和 培训学员使用 NHP、临床研究基础知识和最先进的分析程序。核心将 与路易斯安那州临床和转化科学 (LA CaTS) 等现有 LSUHSC 资源协同作用 中心、动物护理部和路易斯安那州癌症研究中心来实现这些目标。核心 将利用专业知识、经验和资源来保证最佳实践和质量控制， 遵守监管指南。集中 CARC 实验和分析活动可确保严谨性、 人类和临床前研究的设计和实施的完整性和透明度在所有方面都得到维护 CARC 项目。