Advanced Imaging and Instrumentation Core

摘要/摘要(30行) 先进成像和仪器核心的任务将是提供必要的技术技能， 培训和资源，以支持提出的复杂的体内研究项目。目前的U19提案是 这要归功于体内成像、记录和操作技术的最新发展 这使得对清醒行为的小鼠进行实时研究成为可能。特别是，必须利用体内的， 学习运动控制的清醒测量技术，因为人必须能够测量相关的运动 产出和任务绩效。我们的先进成像和仪器核心由以下领域的主要专家组成 新技术的开发和部署，其中两项是大脑倡议获奖者。然而， 重要的是要注意，在这里部署的技术不需要重大的高风险创新， 而是代表了最近展示的突破性技术的集成和实施 严格应用于电机电路的研究。其中包括3光子活体功能显微镜，自适应 光学辅助的光操纵、广域光学测绘和中尺度双光子显微镜。这些 成像方法将与创新的手术准备、任务、电极记录和 闭环系统战略。重要的是，这些技术都不是商业上可用的，因此核心将 需要确保在整个过程中快速有效地建立和支持这些系统 项目的持续时间。记录这一发展的同时，将采用不断发展的 新技术的出现。先进的成像和仪器核心也将发挥作用 在培训和使研究人员能够采用复杂技术方面发挥重要作用，同时与 数据科学资源核心-数据标准化、归档、分析和传播的方方面面。 总体而言，这一核心将代表专家之间在开发和部署 新技术，与那些利用这些重要系统的力量的人合作。

Summary/Abstract (30 lines) The mission of the Advanced Imaging and Instrumentation Core will be to deliver the necessary technical skill, training and resources to enable the complex in-vivo research projects proposed. The current U19 proposal is only possible thanks to very recent developments in in-vivo imaging, recording and manipulation techniques that have enabled real-time studies in awake behaving mice. In particular, it is essential to leverage in-vivo, awake measurement techniques to study motor control since one must be able to measure associated motor output and task performance. Our Advanced Imaging and Instrumentation Core is composed of key experts in the development and deployment of novel technologies, 2 of them BRAIN Initiative grant awardees. However, it is important to note that the technologies to be deployed here will not require significant high-risk innovation, but rather represent the integration and implementation of recently demonstrate breakthrough techniques for rigorous application to studying motor circuits. These include 3-photon in-vivo functional microscopy, Adaptive optics assisted photo manipulation, wide-field optical mapping and meso-scale two-photon microscopy. These imaging methods will be combined with innovative surgical preparations, tasks, electrode recordings and closed-loop strategies. Importantly, none of these technologies is available commercially, and thus the core will need to ensure that these systems are quickly and effectively established and supported throughout the duration of the project. Documentation of this development will be accompanied by adoption of ever evolving new technologies as they become available. The Advanced Imaging and Instrumentation Core will also play an important role in training and enabling researcher to adopt complex techniques, while working closely with the Data Science Resource core on all aspects of data standardization, archiving, analysis and dissemination. Overall, this core will represent a deep collaboration between experts in the development and deployment of novel technologies, in collaboration with those leveraging the power of these important systems.