Advanced Imaging and Instrumentation Core

摘要/摘要（30行） 高级成像和仪器核心的使命是提供必要的技术技能， 培训和资源，使复杂的体内研究项目的建议。目前的U19提案是 这要归功于体内成像、记录和操作技术的最新发展 能够对清醒的老鼠进行实时研究。特别是，必须利用体内， 清醒的测量技术来研究运动控制，因为一个人必须能够测量相关的运动 产出和任务绩效。我们的高级成像和仪器核心由以下领域的关键专家组成： 新技术的开发和部署，其中2人获得了BRAIN Initiative奖。然而，在这方面， 重要的是要注意到，这里要部署的技术将不需要重大的高风险创新， 而是代表了最近展示的突破性技术的集成和实现， 严格应用于研究电机电路。这些包括3光子体内功能显微镜，自适应 光学辅助的照片处理，宽场光学测绘和中尺度双光子显微镜。这些 成像方法将与创新的手术准备、任务、电极记录和 闭环策略。重要的是，这些技术都没有商业化，因此核心将 需要确保这些系统迅速有效地建立起来，并在整个 项目的持续时间。在记录这一发展的同时，还将通过不断发展的 新技术的出现。先进成像和仪器核心也将发挥作用， 在培训和使研究人员能够采用复杂技术方面发挥重要作用，同时与 关于数据标准化、归档、分析和传播各个方面的核心资源。 总的来说，这个核心将代表专家们在开发和部署 新技术，与那些利用这些重要系统的力量的人合作。

Summary/Abstract (30 lines) The mission of the Advanced Imaging and Instrumentation Core will be to deliver the necessary technical skill, training and resources to enable the complex in-vivo research projects proposed. The current U19 proposal is only possible thanks to very recent developments in in-vivo imaging, recording and manipulation techniques that have enabled real-time studies in awake behaving mice. In particular, it is essential to leverage in-vivo, awake measurement techniques to study motor control since one must be able to measure associated motor output and task performance. Our Advanced Imaging and Instrumentation Core is composed of key experts in the development and deployment of novel technologies, 2 of them BRAIN Initiative grant awardees. However, it is important to note that the technologies to be deployed here will not require significant high-risk innovation, but rather represent the integration and implementation of recently demonstrate breakthrough techniques for rigorous application to studying motor circuits. These include 3-photon in-vivo functional microscopy, Adaptive optics assisted photo manipulation, wide-field optical mapping and meso-scale two-photon microscopy. These imaging methods will be combined with innovative surgical preparations, tasks, electrode recordings and closed-loop strategies. Importantly, none of these technologies is available commercially, and thus the core will need to ensure that these systems are quickly and effectively established and supported throughout the duration of the project. Documentation of this development will be accompanied by adoption of ever evolving new technologies as they become available. The Advanced Imaging and Instrumentation Core will also play an important role in training and enabling researcher to adopt complex techniques, while working closely with the Data Science Resource core on all aspects of data standardization, archiving, analysis and dissemination. Overall, this core will represent a deep collaboration between experts in the development and deployment of novel technologies, in collaboration with those leveraging the power of these important systems.