A multi-wavelength lighsheet microscope for volumetric imaging

用于体积成像的多波长光片显微镜

• 批准号: 9274623
• 负责人: Darcy S Peterka
• 金额: $ 23.58万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-22 至 2019-03-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274623
• 关键词: Brain; Development; Dimensions; Elements; Genes; Image; Learning; Length; Methods; Microscope; Microscopic; Microscopy; Neurosciences; Performance; Resolution; Role; Shapes; System; Tissues; brain size; brain tissue; cellular imaging; experience; instrument; neural circuit

The brain remains one of the most mysterious systems in the universe – one critical challenge in gaining understanding has been that its constituent elements have features that span across many length scales, in three-dimensions, from the microscopic to the microscopic. In the last few years, new developments in tissue clearing methods and microscopy promise to allow the exploration of the brain over its natural range of scales. We propose to acquire a high performance multi-wavelength lightsheet microscope for a multi-user cellular imaging core that is capable of imaging centimeter size brain and tissue sections while maintaining sub-cellular resolution. This versatile instrument can be used with all currently developed tissue clearing agents, as well as with live tissue, and will be applied to myriad systems that span critical Neuroscience questions, from the role of molecules and genes in development, to the role of experience and learning, in shaping neural circuits.

大脑仍然是宇宙中最神秘的系统之一-一个关键的挑战 在获得理解的过程中，它的组成元素具有跨越 从微观到微观的三维尺度。在过去 几年来，组织清除方法和显微镜的新发展有望使 探索大脑的自然尺度。我们建议获得一个高 用于多用户细胞成像核心的高性能多波长光片显微镜， 能够成像厘米大小的大脑和组织切片，同时保持亚细胞 分辨率这种多功能仪器可用于所有目前开发的组织清除 代理商，以及与活组织，并将被应用于无数的系统，跨越关键 神经科学的问题，从分子和基因在发育中的作用， 经验和学习，在塑造神经回路。

项目成果

Control of non-REM sleep by ventrolateral medulla glutamatergic neurons projecting to the preoptic area.

• DOI: 10.1038/s41467-022-32461-3
• 发表时间: 2022-08-12
• 期刊: Nature communications
• 影响因子: 16.6

Vasculature of the Suprachiasmatic Nucleus: Pathways for Diffusible Output Signals.

• DOI: 10.1177/07487304231189537
• 发表时间: 2023-12
• 期刊: JOURNAL OF BIOLOGICAL RHYTHMS
• 影响因子: 3.5
• 作者: Yao, Yifan;Green, Isabella K.;Taub, Alana B.;Tazebay, Ruya;LeSauter, Joseph;Silver, Rae
• 通讯作者: Silver, Rae