Multi-scale enhanced sampling of disordered proteins
无序蛋白质的多尺度增强采样
基本信息
- 批准号:9379858
- 负责人:
- 金额:$ 26.84万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-01-01 至 2020-11-30
- 项目状态:已结题
- 来源:
- 关键词:AffectAlgorithmsAutomobile DrivingBindingBiologicalBiophysicsCancer BiologyCancer InterventionCancer PrognosisClinical ResearchComplexCoupledCouplingDiseaseDisease PathwayEquilibriumGenotoxic StressGoalsGrainHumanInterventionKineticsKnowledgeLinkMDM2 geneMalignant NeoplasmsMediatingMethodsModelingMolecularMolecular ConformationMutateMutationN-terminalNatureOutcomePatientsPeptidesPharmacotherapyPhosphorylationPlayPost-Translational Protein ProcessingPropertyProtein ConformationProtein p53ProteinsProtocols documentationResearchResolutionRoleSamplingSequence AnalysisSideSignal TransductionStructureStructure-Activity RelationshipTP53 geneTechniquesTemperatureTestingThermodynamicsTimeTransactivationTranscription CoactivatorTumor SuppressionVertebral columnadvanced simulationapplied biomedical researchbasebiophysical analysiscancer statisticscomparativecomputer studiescomputerized toolsconformational conversiondesignexperienceexperimental studymalignant breast neoplasmmodel designmutantnovelpublic health relevanceresponserestraintsimulationsmall moleculetreatment responsetumorigenesis
项目摘要
DESCRIPTION (provided by applicant): The tumor suppressor p53 is the most frequently mutated protein in human cancers. Clinical studies of breast cancer have suggested that the type of p53 mutation can be linked to cancer prognosis, response to drug treatment, and patient survival. It is thus crucial to understand the molecular basis of p53 inactivation by various types
of mutations, so as to understand the biological consequences and assess potential cancer intervention strategies. This project contributes to this important goal by determining the structural and functional mechanisms of p53 inactivation by cancer mutants in its transactivation domain (TAD). Intriguingly, p53-TAD is an intrinsically disordered protein (IDP). Its properties are governed by a heterogeneous ensemble of conformations that does not lend itself to description using traditional methods that are geared toward describing a coherent set of similar structures. Reliable atomistic simulations have an important and transformative role to play in terms of describing IDP ensembles and their interactions. At the same time, the heterogeneous and dynamic nature of IDPs like p53-TAD also present important challenges that push the limit of the protein force field accuracy and conformational sampling capability. In this project, we wil leverage our extensive experience and recent contributions in developing advanced techniques for atomistic simulation of IDPs and pursue two parallel specific aims. In Aim 1, we will develop novel multi-scale enhanced sampling techniques for efficient and accurate atomistic simulations of IDP ensembles and their interaction. In Aim 2, we will integrate these advanced simulation techniques with NMR and other biophysical experiments to determine the structural and functional consequences of p53-TAD cancer mutations. Specifically, we will test a novel hypothesis that TAD cancer mutations can modulate unbound conformational ensembles, perturb the balance between p53 binding to negative regulator MDM2 and the general transcriptional co-activator CBP, and further alter how this balance is regulated by multisite phosphorylation of p53-TAD. Successful completion of this project will provide cutting-edge computational tools for de novo simulation of protein conformational equilibria and transitions. The proposed research also represents the first systematic study of the biophysical basis of how disease mutants affect the structure-function relationship of p53-TAD, and provides a paradigm for understanding many other IDPs that are key components of cellular regulatory networks and over-represented in major disease pathways.
描述(由申请人提供):肿瘤抑制因子p53是人类癌症中最常见的突变蛋白。乳腺癌的临床研究表明,p53突变的类型可能与癌症预后、对药物治疗的反应和患者生存有关。因此,了解p53失活的分子基础是至关重要的,
突变,以便了解生物学后果和评估潜在的癌症干预策略。该项目有助于这一重要目标,通过确定结构和功能机制的p53失活的癌症突变体在其反式激活域(p53)。有趣的是,p53-p53是一种内在无序蛋白(IDP)。它的性质是由一个异构的整体构象,不适合使用传统的方法,是面向描述一组连贯的类似结构的描述。可靠的原子模拟在描述IDP集成及其相互作用方面发挥着重要的变革性作用。与此同时,像p53-p53这样的IDP的异质性和动态性也提出了重要的挑战,这些挑战推动了蛋白质力场准确性和构象采样能力的极限。在这个项目中,我们将利用我们的丰富经验和最近的贡献,在发展先进的技术,原子模拟的IDP和追求两个平行的具体目标。在目标1中,我们将开发新的多尺度增强采样技术,用于IDP系综及其相互作用的有效和准确的原子模拟。在目标2中,我们将把这些先进的模拟技术与NMR和其他生物物理实验相结合,以确定p53-p53癌症突变的结构和功能后果。具体来说,我们将测试一种新的假设,即肺癌突变可以调节未结合的构象集合,扰乱p53与负调节因子MDM 2和一般转录共激活因子CBP结合之间的平衡,并进一步改变这种平衡是如何通过p53-p53的多位点磷酸化来调节的。该项目的成功完成将为蛋白质构象平衡和转变的从头模拟提供尖端的计算工具。拟议的研究还代表了疾病突变体如何影响p53-p53结构-功能关系的生物物理基础的首次系统研究,并为理解许多其他IDP提供了一个范例,这些IDP是细胞调控网络的关键组成部分,并在主要疾病途径中过度表达。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jianhan Chen其他文献
Jianhan Chen的其他文献
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{{ truncateString('Jianhan Chen', 18)}}的其他基金
Disordered Proteins and Dynamic Interactions in Biology and Diseases.
生物学和疾病中的无序蛋白质和动态相互作用。
- 批准号:
10573331 - 财政年份:2022
- 资助金额:
$ 26.84万 - 项目类别:
Disordered Proteins and Dynamic Interactions in Biology and Diseases.
生物学和疾病中的无序蛋白质和动态相互作用。
- 批准号:
10330292 - 财政年份:2022
- 资助金额:
$ 26.84万 - 项目类别:
Multi-scale enhanced sampling of disordered proteins
无序蛋白质的多尺度增强采样
- 批准号:
9485621 - 财政年份:2016
- 资助金额:
$ 26.84万 - 项目类别:
SIMULATION OF SPONTANEOUS PEPTIDE INSERTION AND ASSEMBLY IN EPITHELIAL MEMBRANES
上皮膜中自发肽插入和组装的模拟
- 批准号:
8167832 - 财政年份:2010
- 资助金额:
$ 26.84万 - 项目类别:
SIMULATION OF SPONTANEOUS PEPTIDE INSERTION AND ASSEMBLY IN EPITHELIAL MEMBRANES
上皮膜中自发肽插入和组装的模拟
- 批准号:
7959802 - 财政年份:2009
- 资助金额:
$ 26.84万 - 项目类别:
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