Husbandry and Management Core

摘要-核心1：畜牧业和管理核心 印度起源的无特定病原体(SPF)恒河猴(Macaca Mulatta)的需求是 NIH在1989年建立SPF育种计划时承认了这一点。持续的需求 这些在艾滋病研究中有价值的动物促进了从2000年开始的U42赠款机制。这个 西南国家灵长类研究中心容纳了两只基因截然不同的SPF恒河猴 殖民地。菌落1代表自2000年以来由SNPRC管理的原始SNPRC殖民地，而 菌落2是在2015年获得的，代表了来自特征良好的SPF菌落的动物 成立于1988年。 畜牧和管理核心的总体目标是生产纯种SPF印度血统 恒河猴为艾滋病相关研究。该核心将与病毒测试核心进行交互，以确保 菌落的SPF状态及其与MHC基因分型核心的最大遗传多样性 增加动物用于艾滋病相关研究的价值，方法是 MHC I类等位基因和遗传变异的特征。由HIGH获得的新的遗传数据 吞吐量测序将提供深入的遗传数据，并显著增加这些数据的价值 爱滋病研究中的动物。 具体目标是： 1.为了最大限度地提高SNPRC SPF育种群体的遗传多样性和生产效率 在基因和生殖方面，将两个群体作为一个群体进行管理，同时进行MHC分型和 基因特征核心。育种计划将被用来避免近亲交配和保持遗传 通过利用关于肥力和繁殖力的数据，将最大限度地提高产量。我们将提供 通过高通量基因组测序更好地描述动物的基因特征。 2.维持760只动物的稳定数量，并提供110只SPF恒河猴用于 每年进行与艾滋病相关的研究。 这些目标的实现将有助于确保印度血统、SPF、恒河猴的供应 足以满足国家艾滋病相关研究项目的需要。

ABSTRACT – Core 1: Husbandry and Management Core The need for specific pathogen free (SPF) rhesus monkeys (Macaca mulatta) of Indian origin was recognized by the NIH when it established the SPF breeding program in 1989. The continued need for these valuable animals in AIDS research facilitated the U42 grant mechanism starting in 2000. The Southwest National Primate Research Center houses two genetically distinct SPF rhesus macaque colonies. Colony 1 represents the original SNPRC colony under SNPRC management since 2000, while Colony 2 was obtained in 2015 and represents animals derived from the well characterized SPF colony initiated in 1988. The overall goals of the Husbandry and Management Core are to produce pedigreed SPF Indian- origin rhesus monkeys for AIDS-related research. This Core will interact with the Viral Testing Core to ensure the SPF status of the colonies and with the MHC Genetic Typing Core to maximize the genetic diversity of the breeding program and to increase the value of the animals for AIDS-related research purposes by characterizing them for MHC Class I alleles and genetic variation. New genetic data obtained by high throughput sequencing will provide in depth genetic data and dramatically increase the value of these animals in AIDS research. The specific aims are: 1. To maximize the genetic diversity and production efficiency of the SNPRC SPF breeding colony by genetically and reproductively managing both colonies as one colony in concert with the MHC Typing and Genetic Characterization Core. Breeding schemes will be used to avoid inbreeding and to maintain genetic variability, while production will be maximized by utilizing data on fertility and fecundity. We will provide greater genetic characterization of animals by high throughput genomic sequencing. 2. To maintain a steady state population of 760 animals and to provide 110 SPF rhesus macaques for AIDS-related research annually. Fulfillment of these aims will contribute to ensuring that the supply of Indian origin, SPF, rhesus monkeys is sufficient to meet the needs of the national AIDS-related research programs.