Husbandry and Management Core

畜牧与管理核心

• 批准号: 10359044
• 负责人: Kenneth Sayers
• 金额: $ 208.17万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10359044
• 关键词: Acquired Immunodeficiency Syndrome; Aging; Alleles; Animals; Birth; Breeding; Censuses; Data; Ensure; Fertility; Funding; Genetic; Genetic Variation; Genomics; Goals; Grant; Health; High-Throughput Nucleotide Sequencing; Inbreeding; MHC Class I Genes; Macaca mulatta; Monkeys; New England; Population; Positioning Attribute; Practice Management; Primates; Production; Productivity; Research; Resources; Scheme; Testing; United States National Institutes of Health; Viral; demographics; genetic pedigree; germ free condition; programs; reproductive; success; surveillance data; young adult

ABSTRACT – Core 1: Husbandry and Management Core The need for specific pathogen free (SPF) rhesus monkeys (Macaca mulatta) of Indian origin was recognized by the NIH when it established the SPF breeding program in 1989. The continued need for these valuable animals in AIDS research facilitated the U42 grant mechanism starting in 2000. The Southwest National Primate Research Center houses two genetically distinct SPF rhesus macaque colonies. Colony 1 represents the original SNPRC colony under SNPRC management since 2000, while Colony 2 was obtained in 2015 and represents animals derived from the well characterized SPF colony initiated in 1988. The overall goals of the Husbandry and Management Core are to produce pedigreed SPF Indian- origin rhesus monkeys for AIDS-related research. This Core will interact with the Viral Testing Core to ensure the SPF status of the colonies and with the MHC Genetic Typing Core to maximize the genetic diversity of the breeding program and to increase the value of the animals for AIDS-related research purposes by characterizing them for MHC Class I alleles and genetic variation. New genetic data obtained by high throughput sequencing will provide in depth genetic data and dramatically increase the value of these animals in AIDS research. The specific aims are: 1. To maximize the genetic diversity and production efficiency of the SNPRC SPF breeding colony by genetically and reproductively managing both colonies as one colony in concert with the MHC Typing and Genetic Characterization Core. Breeding schemes will be used to avoid inbreeding and to maintain genetic variability, while production will be maximized by utilizing data on fertility and fecundity. We will provide greater genetic characterization of animals by high throughput genomic sequencing. 2. To maintain a steady state population of 760 animals and to provide 110 SPF rhesus macaques for AIDS-related research annually. Fulfillment of these aims will contribute to ensuring that the supply of Indian origin, SPF, rhesus monkeys is sufficient to meet the needs of the national AIDS-related research programs.

核心1：畜牧业和管理核心 对无特定病原体（SPF）印度恒河猴（Macaca mulatta）的需求是 在1989年建立SPF育种计划时得到了NIH的认可。是否继续有必要使用 这些在艾滋病研究中有价值的动物促进了2000年开始的U42资助机制。的 西南国家灵长类动物研究中心饲养了两种遗传上不同的SPF恒河猴 殖民地殖民地1代表自2000年以来由SNPRC管理的原始SNPRC殖民地，而 菌落2于2015年获得，代表来自充分表征的SPF菌落的动物 始于1988年。 畜牧业和管理核心的总体目标是生产纯种SPF印度血统 恒河猴进行艾滋病相关研究。该核心将与病毒检测核心相互作用，以确保 菌落的SPF状态和MHC遗传分型核心，以最大限度地提高遗传多样性 的育种计划，并增加动物的价值，用于艾滋病相关的研究目的， 表征它们的MHC I类等位基因和遗传变异。新的基因数据由高 通量测序将提供深入的遗传数据，并大大增加这些数据的价值。 艾滋病研究中的动物 具体目标是： 1.为了最大化SNPRC SPF育种群体的遗传多样性和生产效率， 在遗传和生殖上将两个集落作为一个集落与MHC分型相一致地管理， 基因特征核心。育种计划将用于避免近亲繁殖和保持遗传 通过利用关于生育力和繁殖力的数据，将最大限度地提高产量。我们将提供 通过高通量基因组测序对动物进行更好的遗传表征。 2.维持760只动物的稳定种群，并提供110只SPF恒河猴， 每年进行艾滋病相关研究。 实现这些目标将有助于确保印度原产的SPF恒河猴的供应 足以满足国家艾滋病相关研究计划的需要。