Husbandry and Management Core

畜牧与管理核心

• 批准号: 10094082
• 负责人: Kenneth Sayers
• 金额: $ 208.17万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10094082
• 关键词: Acquired Immunodeficiency Syndrome; Aging; Alleles; Animals; Birth; Breeding; Censuses; Data; Ensure; Fertility; Funding; Genetic; Genetic Variation; Genomics; Goals; Grant; Health; High-Throughput Nucleotide Sequencing; Inbreeding; MHC Class I Genes; Macaca mulatta; Monkeys; New England; Population; Positioning Attribute; Practice Management; Primates; Production; Productivity; Research; Resources; Scheme; Testing; United States National Institutes of Health; Viral; demographics; genetic pedigree; germ free condition; programs; reproductive; success; surveillance data; young adult

ABSTRACT – Core 1: Husbandry and Management Core The need for specific pathogen free (SPF) rhesus monkeys (Macaca mulatta) of Indian origin was recognized by the NIH when it established the SPF breeding program in 1989. The continued need for these valuable animals in AIDS research facilitated the U42 grant mechanism starting in 2000. The Southwest National Primate Research Center houses two genetically distinct SPF rhesus macaque colonies. Colony 1 represents the original SNPRC colony under SNPRC management since 2000, while Colony 2 was obtained in 2015 and represents animals derived from the well characterized SPF colony initiated in 1988. The overall goals of the Husbandry and Management Core are to produce pedigreed SPF Indian- origin rhesus monkeys for AIDS-related research. This Core will interact with the Viral Testing Core to ensure the SPF status of the colonies and with the MHC Genetic Typing Core to maximize the genetic diversity of the breeding program and to increase the value of the animals for AIDS-related research purposes by characterizing them for MHC Class I alleles and genetic variation. New genetic data obtained by high throughput sequencing will provide in depth genetic data and dramatically increase the value of these animals in AIDS research. The specific aims are: 1. To maximize the genetic diversity and production efficiency of the SNPRC SPF breeding colony by genetically and reproductively managing both colonies as one colony in concert with the MHC Typing and Genetic Characterization Core. Breeding schemes will be used to avoid inbreeding and to maintain genetic variability, while production will be maximized by utilizing data on fertility and fecundity. We will provide greater genetic characterization of animals by high throughput genomic sequencing. 2. To maintain a steady state population of 760 animals and to provide 110 SPF rhesus macaques for AIDS-related research annually. Fulfillment of these aims will contribute to ensuring that the supply of Indian origin, SPF, rhesus monkeys is sufficient to meet the needs of the national AIDS-related research programs.

摘要 – 核心 1：畜牧和管理核心 对印度原产的无特定病原体（SPF）恒河猴（Macaca mulatta）的需求是 NIH 在 1989 年建立 SPF 育种计划时得到了认可。 这些在艾滋病研究中有价值的动物促进了 2000 年开始的 U42 资助机制。 西南国家灵长类研究中心饲养着两只基因不同的 SPF 恒河猴 殖民地。 Colony 1 代表自 2000 年以来受 SNPRC 管理的原始 SNPRC 殖民地，而 菌落 2 于 2015 年获得，代表来自已充分表征的 SPF 菌落的动物 1988年开始。 畜牧和管理核心的总体目标是生产源自印度的纯种 SPF 恒河猴用于艾滋病相关研究。该核心将与病毒测试核心交互，以确保 菌落的 SPF 状态以及 MHC 遗传分型核心可最大限度地提高遗传多样性 育种计划并增加动物用于艾滋病相关研究目的的价值 表征它们的 MHC I 类等位基因和遗传变异。高新获得的基因数据 高通量测序将提供深入的遗传数据并显着提高这些数据的价值 艾滋病研究中的动物。 具体目标是： 1. 最大限度地提高SNPRC SPF育种群体的遗传多样性和生产效率 与 MHC 分型一致，对两个菌落作为一个菌落进行遗传和生殖管理 遗传表征核心。育种计划将用于避免近亲繁殖并保持遗传 变异性，而通过利用生育力和繁殖力数据可以最大限度地提高产量。我们将提供 通过高通量基因组测序对动物进行更深入的遗传表征。 2. 维持760只动物的稳定数量，并提供110只SPF级恒河猴 每年与艾滋病相关的研究。 这些目标的实现将有助于确保印度原产 SPF 恒河猴的供应 足以满足国家艾滋病相关研究计划的需要。