Research Supplements to Promote Diversity in Health-Related Research

项目总结 这是一个行政补充应用程序，旨在促进研究生产力和 一位有前途的少数民族博士后的职业培训。这一假说是建立在一个新的 考生观察潘氏细胞溶菌酶的分泌如何被调节。 拟议的实验将对小鼠肠腔中的溶菌酶分泌量进行生化量化。 缺乏Rab11b交易的小鼠。其他拟议的实验将揭示Paneth细胞特异性 Rab11b相互作用组，可能有助于揭示抗菌肽的新调控机制 分泌物。该项目将加强和扩大家长奖的总体目标，即 研究肠道溶菌酶对共生微生物区系的粘膜免疫反应的贡献。 候选人目前是罗格斯大学PI实验室的全日制博士后研究员。这个 候选人近十年的学术道路令人信服地展示了强大的动力和 坚持不懈地从事实验室研究和生物医学科学研究。该项目 是很重要的，因为肠道溶菌酶水平的升高及其由结肠粘膜异位产生的 在临床活动性肠炎条件下观察到。更好地了解溶菌酶如何 分泌被调节将使人们对经常观察到的有缺陷的溶菌酶包装和 克罗恩病(CD)患者潘氏细胞的分泌物。该项目是多学科性质的， 依赖于实验性结肠炎、粘膜免疫学、生理学研究、遗传学、 和肠道上皮生物学。因此，该项目非常适合培养需要的博士生 较强的协作能力和科学沟通能力。职业发展和辅导 制定了连贯的计划，以促进博士论文的顺利完成和 由候选人过渡到博士后研究。

PROJECT SUMMARY This is an administrative supplement application that aims to promote the research productivity and career training of a promising minority pre-doctoral fellow. The hypothesis was built on a new observation made by the candidate in terms of how Paneth cell lysozyme secretion may be regulated. The proposed experiments will biochemically quantify lysozyme secretion from mouse intestinal lumen of mice lacking Rab11b trafficking. Additional proposed experiments will uncover Paneth cell specific Rab11b interactome, which may shed lights on novel regulatory mechanism for antimicrobial peptide secretion. The project will enhance and expand the overarching goal of the parent award that investigates contribution of intestinal lysozyme to mucosal immune response to commensal microbiota. The candidate is currently a full-time predoctoral fellow in the PI’s laboratory at Rutgers University. The candidate’s academic path in the last ten years convincingly demonstrated a strong motivation and persistent endeavor in pursuing laboratory research and biomedical science as a career. The project is important as elevated levels of intestinal lysozyme and its ectopic production by colonic mucosa are observed in clinically active intestinal inflammation conditions. A better understanding of how lysozyme secretion is regulated will bring insights into the frequently observed defective lysozyme packaging and secretion in Paneth cells of Crohn’s disease (CD) patients. The project is multidisciplinary in nature and relies on different expertise in experimental colitis, mucosal immunology, gnotobiotics study, genetics, and intestinal epithelial biology. Thus, the project is ideal for training of a Ph.D. candidate as it requires strong collaborative activities and scientific communication skills. Career development and mentoring plans are cohesively put in place to facilitate a successful completion of the Ph.D. dissertation and a transition into postdoctoral research by the candidate.