Research Supplements to Promote Diversity in Health-Related Research

项目摘要 这是一个行政补充申请，旨在促进研究生产力， 一个有前途的少数民族博士前研究员的职业培训。这个假设是建立在一个新的 候选人在潘氏细胞溶菌酶分泌如何被调节方面所做的观察。 拟议的实验将生物化学定量溶菌酶分泌从小鼠肠腔 缺乏Rab11b运输的小鼠。其他拟议的实验将揭示潘氏细胞特异性 Rab11b相互作用体可能揭示抗菌肽的新调控机制 分泌物该项目将加强和扩大家长奖的总体目标， 研究肠溶菌酶对肠道微生物群的粘膜免疫应答的贡献。 候选人目前是罗格斯大学PI实验室的全职博士前研究员。的 候选人在过去十年的学术道路令人信服地证明了强烈的动机， 以实验室研究和生物医学科学为职业的不懈奋进。项目 是重要的，因为肠溶菌酶水平的升高及其由结肠粘膜异位产生， 在临床活动性肠道炎症条件下观察到。更好地理解溶菌酶 分泌的调节将使人们深入了解经常观察到的有缺陷的溶菌酶包装， 克罗恩病（CD）患者潘氏细胞分泌物。该项目具有多学科性质， 依赖于实验性结肠炎，粘膜免疫学，gnotobiotics研究，遗传学， 和肠上皮生物学。因此，该项目是培养博士的理想选择。候选人，因为它需要 较强的协作能力和科学沟通能力。职业发展和辅导 计划是凝聚到位，以促进成功完成博士学位。论文和 由候选人转入博士后研究。

PROJECT SUMMARY This is an administrative supplement application that aims to promote the research productivity and career training of a promising minority pre-doctoral fellow. The hypothesis was built on a new observation made by the candidate in terms of how Paneth cell lysozyme secretion may be regulated. The proposed experiments will biochemically quantify lysozyme secretion from mouse intestinal lumen of mice lacking Rab11b trafficking. Additional proposed experiments will uncover Paneth cell specific Rab11b interactome, which may shed lights on novel regulatory mechanism for antimicrobial peptide secretion. The project will enhance and expand the overarching goal of the parent award that investigates contribution of intestinal lysozyme to mucosal immune response to commensal microbiota. The candidate is currently a full-time predoctoral fellow in the PI’s laboratory at Rutgers University. The candidate’s academic path in the last ten years convincingly demonstrated a strong motivation and persistent endeavor in pursuing laboratory research and biomedical science as a career. The project is important as elevated levels of intestinal lysozyme and its ectopic production by colonic mucosa are observed in clinically active intestinal inflammation conditions. A better understanding of how lysozyme secretion is regulated will bring insights into the frequently observed defective lysozyme packaging and secretion in Paneth cells of Crohn’s disease (CD) patients. The project is multidisciplinary in nature and relies on different expertise in experimental colitis, mucosal immunology, gnotobiotics study, genetics, and intestinal epithelial biology. Thus, the project is ideal for training of a Ph.D. candidate as it requires strong collaborative activities and scientific communication skills. Career development and mentoring plans are cohesively put in place to facilitate a successful completion of the Ph.D. dissertation and a transition into postdoctoral research by the candidate.